Hydrophobic Polyampholytes and Nonfreezing Cold Temperature Stimulate Internalization of Au Nanoparticles to Zwitterionic Liposomes

Ahmed, Sana; Matsumura, Kazuaki; Hamada, Tsutomu

doi:10.1021/acs.langmuir.8b00920

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…The higher the hydrophobicity of a nanoparticle, the greater the affinity of the particle to the bacterial membranes [48]. Previous studies also presented that Au nanoparticles modified with hydrophobic polyampholytes could be adsorbed onto DOPC liposomes [49]. Thus, the PS-NH 2 was bound to the liposome via electrostatic and hydrophobic interactions, leading to the formation of lipid-coated PS-NH 2 .…”

Section: Internalised Ps-nh 2 Form Lipid-coated Nanoplasticsmentioning

confidence: 99%

Distinct lipid membrane interaction and uptake of differentially charged nanoplastics in bacteria

Dai

Huang

et al. 2022

J Nanobiotechnol

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Background Nanoplastics have been recently found widely distributed in our natural environment where ubiquitously bacteria are major participants in various material cycles. Understanding how nanoplastics interact with bacterial cell membrane is critical to grasp their uptake processes as well as to analyze their associated risks in ecosystems and human microflora. However, little is known about the detailed interaction of differentially charged nanoplastics with bacteria. The present work experimentally and theoretically demonstrated that nanoplastics enter into bacteria depending on the surface charges and cell envelope structural features, and proved the shielding role of membrane lipids against nanoplastics. Results Positively charged polystyrene nanoplastics (PS-NH2, 80 nm) can efficiently translocate across cell membranes, while negatively charged PS (PS-COOH) and neutral PS show almost no or much less efficacy in translocation. Molecular dynamics simulations revealed that the PS-NH2 displayed more favourable electrostatic interactions with bacterial membranes and was subjected to internalisation through membrane penetration. The positively charged nanoplastics destroy cell envelope of Gram-positive B. subtilis by forming membrane pore, while enter into the Gram-negative E. coli with a relatively intact envelope. The accumulated positively charged nanoplastics conveyed more cell stress by inducing a higher level of reactive oxygen species (ROS). However, the subsequently released membrane lipid-coated nanoplastics were nearly nontoxic to cells, and like wise, stealthy bacteria wrapped up with artifical lipid layers became less sensitive to the positively charged nanoplastics, thereby illustrating that the membrane lipid can shield the strong interaction between the positively charged nanoplastics and cells. Conclusions Our findings elucidated the molecular mechanism of nanoplastics’ interaction and accumulation within bacteria, and implied the shielding and internalization effect of membrane lipid on toxic nanoplastics could promote bacteria for potential plastic bioremediation. Graphical Abstract

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Section: Internalised Ps-nh 2 Form Lipid-coated Nanoplasticsmentioning

confidence: 99%

Distinct lipid membrane interaction and uptake of differentially charged nanoplastics in bacteria

Dai

Huang

et al. 2022

J Nanobiotechnol

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Construction Strategy of Functionalized Liposomes and Multidimensional Application

Wang,

Lan,

Zhu

et al. 2024

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Liposomes are widely used in the biological field due to their good biocompatibility and surface modification properties. With the development of biochemistry and material science, many liposome structures and their surface functional components have been modified and optimized one by one, pushing the liposome platform from traditional to functionalized and intelligent, which will better satisfy and expand the needs of scientific research. However, a main limiting factor effecting the efficiency of liposomes is the complicated environmental conditions in the living body. Currently, in order to overcome the above problem, functionalized liposomes have become a very promising strategy. In this paper, binding strategies of liposomes with four main functional elements, namely nucleic acids, antibodies, peptides, and stimuli‐responsive motif have been summarized for the first time. In addition, based on the construction characteristics of functionalized liposomes, such as drug‐carrying, targeting, long‐circulating, and stimulus‐responsive properties, a comprehensive overview of their features and respective research progress are presented. Finally, the paper critically presents the limitations of these functionalized liposomes in the current applications and also prospectively suggests the future development directions, aiming to accelerate realization of their industrialization.

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Hydrophobic Polyampholytes and Nonfreezing Cold Temperature Stimulate Internalization of Au Nanoparticles to Zwitterionic Liposomes

Cited by 2 publications

References 45 publications

Distinct lipid membrane interaction and uptake of differentially charged nanoplastics in bacteria

Distinct lipid membrane interaction and uptake of differentially charged nanoplastics in bacteria

Construction Strategy of Functionalized Liposomes and Multidimensional Application

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