Hydrophobicity of abiotic surfaces governs droplets deposition and evaporation patterns

Richard, Elodie; Dubois, Thomas; Jha, Piyush Kumar; Faille, Christine

doi:10.1016/j.fm.2020.103538

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…The inoculation of surfaces with spores was carefully considered to produce a realistic challenge for the decontaminants and processes under investigation. Droplets of spore suspension drying on a surface can result in different spore deposition patterns depending on a range of factors that include droplet composition, size and shape (Richard et al, 2020 ), which can in turn affect decontaminant mass‐transport to the spore (solubility and diffusion) (Richard et al, 2020 ). Drying of larger droplets such as those that may be dispensed by pipetting can result in bacteria redistribution and aggregation to its peripheral ‘ring’ (Richard et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Droplets of spore suspension drying on a surface can result in different spore deposition patterns depending on a range of factors that include droplet composition, size and shape (Richard et al, 2020 ), which can in turn affect decontaminant mass‐transport to the spore (solubility and diffusion) (Richard et al, 2020 ). Drying of larger droplets such as those that may be dispensed by pipetting can result in bacteria redistribution and aggregation to its peripheral ‘ring’ (Richard et al, 2020 ). Consequently, spores positioned internally within the aggregate may receive a lower dose of the active substance compared with relatively more dispersed spores from smaller droplets deposited by aerosol nebulization.…”

Section: Discussionmentioning

confidence: 99%

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Dose–response analysis of Bacillus thuringiensis HD-1 cry- spore reduction on surfaces using formaldehyde with pre-germination

Gazi

Bayliss

O'Sullivan³

et al. 2022

Journal of Applied Microbiology

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Aim To establish a basis for rapid remediation of large areas contaminated with Bacillus anthracis spores. Methods and Results Representative surfaces of wood, steel and cement were coated by nebulization with B. thuringiensis HD‐1 cry‐ (a simulant for B. anthracis) at 5.9 ± 0.2, 6.3 ± 0.2 and 5.8 ± 0.2 log10 CFU per cm2, respectively. These were sprayed with formaldehyde, either with or without pre‐germination. Low volume (equivalent to ≤2500 L ha−1) applications of formaldehyde at 30 g l−1 to steel or cement surfaces resulted in ≥4 or ≤2 log10 CFU per cm2 reductions respectively, after 2 h exposure. Pre‐germinating spores (500 mmol l−1 l‐alanine and 25 mmol l−1 inosine, pH 7) followed by formaldehyde application showed higher levels of spore inactivation than formaldehyde alone with gains of up to 3.4 log10 CFU per cm2 for a given dose. No loss in B. thuringiensis cry‐ viability was measured after the 2 h germination period, however, a pre‐heat shock log10 reduction was seen for B. anthracis strains: LSU149 (1.7 log10), Vollum and LSU465 (both 0.9 log10), LSU442 (0.2 log10), Sterne (0.8 log10) and Ames (0.6 log10). Conclusions A methodology was developed to produce representative spore contamination of surfaces along with a laboratory‐based technique to measure the efficacy of decontamination. Dose–response analysis was used to optimize decontamination. Pre‐germinating spores was found to increase effectiveness of decontamination but requires careful consideration of total volume used (germinant and decontaminant) by surface type. Significance and Impact of the Study To be practically achievable, decontamination of a wide area contaminated with B. anthracis spores must be effective, timely and minimize the amount of materials required. This study uses systematic dose–response methodology to demonstrate that such an approach is feasible.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dose–response analysis of Bacillus thuringiensis HD-1 cry- spore reduction on surfaces using formaldehyde with pre-germination

Gazi

Bayliss

O'Sullivan³

et al. 2022

Journal of Applied Microbiology

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show abstract

“…Surface topography- In addition to the environmental conditions described above, the surface features such as hydrophobicity and surface roughness can have a significant impact on how long moisture stays on its surface [ 85 ]. While a hydrophilic surface allows water to spread out evenly over the whole surface and to dry evenly, a hydrophobic surface results in the formation of droplets that cover the surface only in parts and that will dry out slower compared to the same volume when place on a hydrophilic material.…”

Section: Incubation/environmental Factors Affecting the Efficacy Of Antimicrobial Test Methodsmentioning

confidence: 99%

How Do We Determine the Efficacy of an Antibacterial Surface? A Review of Standardised Antibacterial Material Testing Methods

Cunliffe

Askew²,

Stephan

et al. 2021

Antibiotics

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Materials that confer antimicrobial activity, be that by innate property, leaching of biocides or design features (e.g., non-adhesive materials) continue to gain popularity to combat the increasing and varied threats from microorganisms, e.g., replacing inert surfaces in hospitals with copper. To understand how efficacious these materials are at controlling microorganisms, data is usually collected via a standardised test method. However, standardised test methods vary, and often the characteristics and methodological choices can make it difficult to infer that any perceived antimicrobial activity demonstrated in the laboratory can be confidently assumed to an end-use setting. This review provides a critical analysis of standardised methodology used in academia and industry, and demonstrates how many key methodological choices (e.g., temperature, humidity/moisture, airflow, surface topography) may impact efficacy assessment, highlighting the need to carefully consider intended antimicrobial end-use of any product.

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“…A recently published paper offers fundamental insights into pattern formation, and links these crack patterns with the underlying mechanical instabilities [156]. on Stainless steel 2B, 2B-SB, Glass slides, and Polypropylene, adopted from [162].…”

Section: Drying Of Microbial Dropletsmentioning

confidence: 99%

“…To fill this gap, Richard et al [162] examined the overall effects of the bacterial spores by uniting various surface properties, such as topography, hydrophilicity or hydrophobicity, particle properties (size and shape), drying rates, and the deposited patterns. It was found that topography has a weaker influence than hydrophobicity.…”

Section: Drying Of Microbial Dropletsmentioning

confidence: 99%

Drying of bio-colloidal sessile droplets: Advances, applications, and perspectives

Pal

Gope

Sengupta

2023

Advances in Colloid and Interface Science

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Hydrophobicity of abiotic surfaces governs droplets deposition and evaporation patterns

Cited by 6 publications

References 38 publications

Dose–response analysis of Bacillus thuringiensis HD-1 cry- spore reduction on surfaces using formaldehyde with pre-germination

Dose–response analysis of Bacillus thuringiensis HD-1 cry- spore reduction on surfaces using formaldehyde with pre-germination

How Do We Determine the Efficacy of an Antibacterial Surface? A Review of Standardised Antibacterial Material Testing Methods

Drying of bio-colloidal sessile droplets: Advances, applications, and perspectives

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