Hydrophobizing Effect of Cations on Acidic Phospholipid Membranes

Mirza, Μ. Y.; Guo, Yao; Arnold, Klaus; Oss, Carel J. van; Ohki, Shinpei

doi:10.1080/01932699808913225

Cited by 26 publications

(25 citation statements)

References 20 publications

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“…Note that the POPC/POPS membrane structure is also more complex, with POPS mostly buried below the choline groups of POPC. Contrary to previous report23, no lateral demixing of PC and PS was observed.…”

Section: Resultscontrasting

confidence: 99%

“…Bilayer dehydration was also reported in earlier MD and experimental studies (e.g. refs 17, 23, 24, 25, 26). …”

Section: Resultssupporting

confidence: 81%

“…It is generally accepted that the presence of Ca 2+ rigidifies and orders lipid bilayers131516171819. Conformational changes of the lipid headgroup region2021, ordering of acyl chains202122, and lipid dehydration1723242526 were reported.…”

mentioning

confidence: 99%

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The complex nature of calcium cation interactions with phospholipid bilayers

Melcrová

Pokorná

Pullanchery

et al. 2016

Sci Rep

240

288

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Understanding interactions of calcium with lipid membranes at the molecular level is of great importance in light of their involvement in calcium signaling, association of proteins with cellular membranes, and membrane fusion. We quantify these interactions in detail by employing a combination of spectroscopic methods with atomistic molecular dynamics simulations. Namely, time-resolved fluorescent spectroscopy of lipid vesicles and vibrational sum frequency spectroscopy of lipid monolayers are used to characterize local binding sites of calcium in zwitterionic and anionic model lipid assemblies, while dynamic light scattering and zeta potential measurements are employed for macroscopic characterization of lipid vesicles in calcium-containing environments. To gain additional atomic-level information, the experiments are complemented by molecular simulations that utilize an accurate force field for calcium ions with scaled charges effectively accounting for electronic polarization effects. We demonstrate that lipid membranes have substantial calcium-binding capacity, with several types of binding sites present. Significantly, the binding mode depends on calcium concentration with important implications for calcium buffering, synaptic plasticity, and protein-membrane association.

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Section: Resultscontrasting

confidence: 99%

“…Bilayer dehydration was also reported in earlier MD and experimental studies (e.g. refs 17, 23, 24, 25, 26). …”

Section: Resultssupporting

confidence: 81%

See 1 more Smart Citation

The complex nature of calcium cation interactions with phospholipid bilayers

Melcrová

Pokorná

Pullanchery

et al. 2016

Sci Rep

240

288

View full text Add to dashboard Cite

show abstract

“…a different form of aggregates: micelles, vesicles or liposomes. Various mechanisms of enzymes adsorption at the surface of aggregated substrate particles (oil droplets, lipid bilayers or monomolecular lipid films) can result from the substrate specificity [13][14][15][16][17]. After adding lipase, electrostatic repulsion may occur between the negatively-charged active site of the enzyme and the ionized fatty acids originating from hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

“…The phospholipases are activated at the interface between the water and phospholipid layer surfaces. Much higher phospholipases enzyme activity is observed at such interfaces as vesicles, monolayers or bilayers than in isotropically dispersed phospholipids [16,17] Phospholipid monolayers are used as model systems to study in general stereospecific reactions, which enable structural control at the living cells interfaces. The results observed for the hydrolysis of typical phospholipids (DPPC or DOPC) proved that the systems with PLA 2 are very sensitive to the conditions and experiment environment because of its stereoselectively.…”

Section: Introductionmentioning

confidence: 99%

Effect of phospholipid and (phospho)lipase modification on interfacial properties of oil/water emulsion

Więcek

2015

Annales UMCS, Chemia

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This review paper focused on the effect of typical phosphorlipid (or lecithin) and enzyme modification on electrokinetic parameters of oil/water emulsion. Physicochemical properties of the systems were investigated taking into account the effective diameter of the droplets as well as the zeta potentials using the dynamic light scattering technique. The effect of phospholipid and phospholipase modification on interfacial properties of o/w emulsion was examined as a function of temperature, pH and ionic strength (effect of Na + or Ca 2+ ions which occur in the physiological fluids). The particular role of Ca 2+ ions in the dispersions with zwitterionic phospholipids (the main components of biological membrane) was confirmed.The phospholipids dipalmitoylphosphatidylcholine, DPPC or dioleoylphosphatidylcholine, DOPC having the same headgroup bound to the apolar tail composed of two saturated or unsaturated chains were used as stabilizing agents. The effective diameters do not always correlate with the zeta potentials. A possible reason for such behaviour might a mechanism different from the electrostatic stabilization. Phospholipids and their mixtures (e.g. lecithin) may undergo spontaneous aggregation in aqueous solution and selfBrought to you by | Florida Atlantic University Authenticated Download Date | 7/28/15 4:59 AM Agnieszka Ewa Wi cek 80 organize into liposomes, which possess different sizes and surface affinities. These unique behaviours of phospholipid dispersion can be controlled using the investigated parameters. These findings are expected to increase in importance as phospholipid systems see more use in self-assembly applications.The other aim of the paper was the comparison of the enzyme phospholipase influence on lipid hydrolysis in the o/w emulsion environment. The work is the study which presents the twofold effect of ethanol dipoles on phosholipid hydrolysis. It is believed that the enzyme effect on the phospholipid aggregation behaviour at the oil-water interface will be helpful for understanding different biological phenomena.

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Young modulus of supported lipid membranes containing milk sphingomyelin in the gel, fluid or liquid-ordered phase, determined using AFM force spectroscopy

Et-Thakafy¹,

Guyomarc’H²,

Lopez³

2019

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The biological membrane surrounding milk fat globules (MFGM) exhibits lateral phase separation of lipids, interpreted as gel or liquid-ordered phase sphingomyelin-rich (milk SM) domains dispersed in a fluid continuous lipid phase. The objective of this study was to investigate whether changes in the phase state of milk SM-rich domains induced by temperature (T < Tm or T > Tm) or cholesterol affected the Young modulus of the lipid membrane. Supported lipid bilayers composed of MFGM polar lipids, milk SM or milk SM/cholesterol (50:50 mol) were investigated at 20°C and 50°C using atomic force microscopy (AFM) and force spectroscopy. At 20°C, gel-phase SM-rich domains and the surrounding fluid phase of the MFGM polar lipids exhibited Young modulus values of 10-20 MPa and 4-6 MPa, respectively. Upon heating at 50°C, the milk SM-rich domains in MFGM bilayers as well as pure milk SM bilayers melted, leading to the formation of a homogeneous membrane with similar Young modulus values to that of a fluid phase (0-5 MPa). Upon addition of cholesterol to the milk SM to reach 50:50 mol%, membranes in the liquid-ordered phase exhibited Young modulus values of a few MPa, at either 20 or 50°C. This indicated that the presence of cholesterol fluidized milk SM membranes and that the Young modulus was weakly affected by the temperature. These results open perspectives for the development of milk polar lipid based vesicles with modulated mechanical properties.

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Hydrophobizing Effect of Cations on Acidic Phospholipid Membranes

Cited by 26 publications

References 20 publications

The complex nature of calcium cation interactions with phospholipid bilayers

The complex nature of calcium cation interactions with phospholipid bilayers

Effect of phospholipid and (phospho)lipase modification on interfacial properties of oil/water emulsion

Young modulus of supported lipid membranes containing milk sphingomyelin in the gel, fluid or liquid-ordered phase, determined using AFM force spectroscopy

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