Hydrostatic pressure enhances mitomycin C induced apoptosis in urothelial carcinoma cells

Chen, Shao Kuan; Chung, C. A.; Cheng, Yu; Huang, Chin Chou; Ruaan, Ruoh‐Chyu; Chen, Wen Yih; Li, Chuan; Tsao, Chia Wen; Hu, Wei; Chien, Chih Cheng

doi:10.1016/j.urolonc.2012.09.004

Cited by 12 publications

(6 citation statements)

References 30 publications

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“…Mitomycin C (MMC; medac GmbH; Wedel, Germany), an alkylating chemotherapy which binds covalently to the DNA, leads to cross-linking and therefor inhibits the replication of the DNA 28 was obtained from the local university pharmacy at a concentration of 1 mg/ml. Due to data of preliminary works [29][30][31] and our own experiments we used MMC in a concentration of 10 µg/ml for all further experiments.…”

Section: Chemotherapymentioning

confidence: 99%

Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Hegmann

Sturm

Niegisch

et al. 2022

Journal of Photochemistry and Photobiology B: Biology

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Section: Chemotherapymentioning

confidence: 99%

Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Hegmann

Sturm

Niegisch

et al. 2022

Journal of Photochemistry and Photobiology B: Biology

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“…In lung cancer cells (CL1-5 cells and A549 cells), 27 cmH 2 O HP increases migration speed and cell volume with the elevation of the expression of various proteins including AQP1, Snail, vinculin and caveolin-1 [107,108]. In urothelial carcinoma cells, 102–1020 cmH 2 O HP promotes apoptosis with the elevation of the expression of Fas ligand, toll-like receptor 6 and connective tissue growth factor in the presence of mitomycin C [109,110]. In addition, extremely high HP more than 1 million cmH 2 O induces immunogenic cell death in tumor cells and is used for the method in cancer immunotherapy [111,112].…”

Section: Effects Of Hp On the Epithelia And Endotheliamentioning

confidence: 99%

Regulation of Epithelial Cell Functions by the Osmolality and Hydrostatic Pressure Gradients: A Possible Role of the Tight Junction as a Sensor

Tokuda

2019

IJMS

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Epithelia act as a barrier to the external environment. The extracellular environment constantly changes, and the epithelia are required to regulate their function in accordance with the changes in the environment. It has been reported that a difference of the environment between the apical and basal sides of epithelia such as osmolality and hydrostatic pressure affects various epithelial functions including transepithelial transport, cytoskeleton, and cell proliferation. In this paper, we review the regulation of epithelial functions by the gradients of osmolality and hydrostatic pressure. We also examine the significance of this regulation in pathological conditions especially focusing on the role of the hydrostatic pressure gradient in the pathogenesis of carcinomas. Furthermore, we discuss the mechanism by which epithelia sense the osmotic and hydrostatic pressure gradients and the possible role of the tight junction as a sensor of the extracellular environment to regulate epithelial functions.

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“…A precondition for the clinical application of HHP-treated tumor cells is safe devitalization. The threshold for the complete devitalization of various cancer entities has been reported to be 250–300 MPa [ 4 – 10 , 17 , 20 , 21 , 23 , 26 – 32 ]. However, most studies do not specify a threshold, leaving great uncertainty about the amplitude required to yield complete devitalization (Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

Maletzki,

Freiin Grote,

Kalle

et al. 2023

Cell Death Discov.

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High hydrostatic pressure specifically devitalizes cells and tissues without major changes in their molecular structure. Hence, high hydrostatic pressure may enhance the development of whole-cell anti-tumor vaccines, representing tumor heterogeneity and thus (neo-) antigen diversity. Moreover, safe devitalization of tumor-infiltrated supporting tissue may facilitate reimplantation for functional reconstruction. However, precise high hydrostatic pressure thresholds for safe cancer cell killing are unknown. Here, we show that high hydrostatic pressure of at least 450 MPa is necessary to safely devitalize head and neck squamous cell cancer. A pressure of 300 MPa, which has been used frequently in cancer vaccine preparation, resulted in partial devitalization with 27% live cells in flow cytometry and 4% remaining autofluorescence in cell culture after one week. The remaining cells could form vital tumors in the chorioallantoic membrane assay. In contrast, 450 MPa killed all cells in vitro and prevented tumor outgrowth in ovo. The effectiveness of 450 MPa was attributed to the induction of DNA double-strand breaks, independent of apoptosis, autophagy, or methuosis. Furthermore, 450 MPa continued to induce immunogenic cell death. Our results demonstrate that 450 MPa of high hydrostatic pressure induces safe and sustained devitalization of head and neck cancer cells and tissues. Because of the heterogeneity in pressure resistance, we propose our approach as a starting point for determining the precise thresholds for other cancer entities. Further studies on head and neck cancer should focus on immunological co-cultures, combinations of immune checkpoint inhibition, and accurate anatomical reconstruction with pressure-treated autografts.

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Hydrostatic pressure enhances mitomycin C induced apoptosis in urothelial carcinoma cells

Cited by 12 publications

References 30 publications

Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Enhancement of human bladder carcinoma cell chemosensitivity to Mitomycin C through quasi-monochromatic blue light (λ = 453 ± 10 nm)

Regulation of Epithelial Cell Functions by the Osmolality and Hydrostatic Pressure Gradients: A Possible Role of the Tight Junction as a Sensor

Establishing safe high hydrostatic pressure devitalization thresholds for autologous head and neck cancer vaccination and reconstruction

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