Hydroxamate formation by anthranilate synthetase of Escherichia coli K12

Somerville, Ronald L.; Elford, Roberta M.

doi:10.1016/0006-291x(67)90331-2

Cited by 14 publications

(3 citation statements)

References 16 publications

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“…The hydroxamate assay procedure of Somerville and Elford (20) and Woolfolk, Shapiro, and Stadtman (23) was used to measure the hydroxamic acid formed by anthranilate synthetase. The reaction mixture contained, in a total volume of 0.9 ml: 40 jAmoles of L-glutamine, 80 j,moles of Tris, pH 7.8, 10,umoles of MgCl2, 200 jAmoles of freshly neutralized hydroxylamine, 0.4,Amole of chorismic acid, water, and enzyme to volume.…”

Section: Coonmentioning

confidence: 99%

Physiological and Kinetic Studies with Anthranilate Synthetase of Bacillus alvei

Catena

DeMoss

1970

J Bacteriol

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Anthranilate synthetase from Bacillus alvei was partially purified by ammonium sulfate fractionation and was stabilized by glycerol. The reaction mechanism of the enzyme was found to be sequential with respect to substrate, and the enzyme formed a hydroxamic acid in the absence of Mg++. The Km for chorismic acid was 1.25 X I Presented in part at the Annual Meeting of the American Society for Microbiology, Detroit, Mich., 5-10 May 1968. Part of a thesis submitted by the senior author to the Graduate College of the University of Illinois in partial fulfillment of the requirements for the Ph.D. degree.

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Section: Coonmentioning

confidence: 99%

Physiological and Kinetic Studies with Anthranilate Synthetase of Bacillus alvei

Catena

DeMoss

1970

J Bacteriol

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“…Isolation of y-glutamyl hydroxamate (55,56) following incubation of the enzyme with chorismate, glutamine, and hydroxylamine provides evidence for a y-glutamyl-enzyme thioester. Hydroxamate formation, dependent upon chorismate and indicative of glutaminase, has been detected with anthranilate synthetase from E. coli (57) and P. putida (33).…”

Section: Mechanism Of Glutamine Utilizationmentioning

confidence: 99%

“…However chemical intermediates have not been identified. The only partial reaction detected is glutaminase (20,57) which appears to be associated with the mechanism of glutamine utilization (Section IV). Formation of a-carboxy-a-Ndimethylnitrone by reaction of N-methyl hydroxylamine with the enolpyruvyl group of chorismate or with pyruvate may (55) or may not (56) be enzymatic and is of unknown significance.…”

Section: Conversion Of Chorismate To Anthranilatementioning

confidence: 99%