Hydroxyapatite coating on stainless steel pre-coated with bovine serum albumin at ambient conditions

Nayar, Suprabha; Pramanick, A K; Sharma, Binay Kumar; Mishra, Rahul Kumar; Bansal, Satyendra Kumar; Prajapati, Amardeep; Sahu, Kaushal Kishor; Das, Swapan K; Pathak, L C; Sinha, Arvind

doi:10.1016/j.colsurfb.2005.09.006

Cited by 7 publications

(6 citation statements)

References 2 publications

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“…The performance of the cysteine coating is compared to stainless steel and gold surfaces. Figure A illustrates the BSA cluster on the stainless steel surface demonstrating the characteristic spherical clusters similar to the published SEM − and AFM images . Figure D illustrates the SEM image of stainless steel (SS) subsequent to BSA exposure.…”

Section: Resultssupporting

confidence: 59%

“…Figure 3A illustrates the BSA cluster on the stainless steel surface demonstrating the characteristic spherical clusters similar to the published SEM 48−50 and AFM images. 51 Figure 3D illustrates the SEM image of stainless steel (SS) subsequent to BSA exposure. Although the stainless steel disks appears well polished on a macroscopic level, SEM images returned cracks with rough grain-like structures that are capable of trapping BSA clusters (Figure 3A).…”

Section: ■ Materials/methodsmentioning

confidence: 99%

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Nonfouling Property of Zwitterionic Cysteine Surface

et al. 2014

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Applications of implantable bioelectronics for analytical and curative purposes are currently limited by their poor long-term biofunctionality in physiological media and nonspecific interactions with biomolecules. In an attempt to prolong in vivo functionality, recent advances in surface modifications have demonstrated that zwitterionic coatings can rival the performance of conventional poly(ethylene glycol) polymers in reducing nonspecific protein fouling. Herein, we report the fabrication of a very thin layer of nonfouling zwitterionic cysteine surface capable of protecting implantable bioelectronics from nonspecific adsorption of plasma proteins. This work is the first of its kind to fabricate, through solution chemistry, a cysteine surface exhibiting zwitterionic state as high as 88% and to demonstrate antibiofouling under the exposure of bovine serum albumin (BSA) and human serum. The fabricated surface utilized a minimal amount of gold substrate, approximately 10 nm, and an extremely thin antifouling layer at 1.14 nm verified by ellipsometry. X-ray photoelectron spectroscopy assessment of the nitrogen (N1s) and carbon (C1s) spectra conclude that 87.8% of the fabricated cysteine surface is zwitterionic, 2.5% is positively charged, and 9.6% is noncharged. Antibiofouling performance of the cysteine surface is quantitatively determined by bicinchoninic acid (BCA) protein assay as well as qualitatively confirmed using scanning electron spectroscopy. Cysteine surfaces demonstrated a BSA fouling of 3.9 ± 4.84% μg/cm(2), which is 93.6% and 98.5% lower than stainless steel and gold surfaces, respectively. Surface plasmon resonance imaging analysis returned similar results and suggest that a thinner cysteine coating will enhance performance. Scanning electron microscopy confirmed the results of BCA assay and suggested that the cysteine surface demonstrated a 69% reduction to serum fouling. The results reported in this paper demonstrate that it is possible to achieve a highly zwitterionic surface through solution chemistry on a macroscopic level that is capable of improving biocompatibility of long-term implantable bioelectronics.

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Section: Resultssupporting

confidence: 59%

Section: ■ Materials/methodsmentioning

confidence: 99%

Nonfouling Property of Zwitterionic Cysteine Surface

et al. 2014

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“…Coating stainless steel with HA material prevents its corrosion in body fluid and makes it biocompatible for bone growth in orthopeadics applications. Many studies applied HA coatings on stainless steel implants [28][29][30]. One study [28] deposited HA coating on 316 SS by dip-coating technique and examined the microstructure and corrosion resistance of the coating.…”

Section: Introductionmentioning

confidence: 99%

“…The results indicated that the coated stainless steel is good candidate for biomedical applications. Suprabha Nayar et al [29] used the protein bovine serum albumin for pre-conditioning the stainless steel substrate before deposition of HA. The protein used has dual nature in both metal binding and a strong affinity for calcium ions.…”

Section: Introductionmentioning

confidence: 99%

Influence of spray parameters on the microstructure and mechanical properties of gas-tunnel plasma sprayed hydroxyapatite coatings

Morks¹,

Kobayashi²

2007

Materials Science and Engineering: B

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“…The only phase identified in the film deposited on 316L stainless steel substrate was hydroxyapatite. This method allows a good control of composition and crystallization of hydroxyapatite films at low temperatures [32,33]. , which produce local effects in the body and destroy the implant [34].…”

Section: Hydroxyapatite -Advances In Composite Nanomaterials Biomedimentioning

confidence: 99%

316L Stainless Steel/Hydroxyapatite Composite Materials for Biomedical Applications

Poinescu¹,

Ion²

2018

Hydroxyapatite - Advances in Composite Nanomaterials, Biomedical Applications and Its Technological Facets

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Hydroxyapatite (HAp) is known as one of the most important biomaterials used for orthopedic and dental applications due to chemical composition similar to that of bone and bioactive properties. However, due to its reduced resistance, HAp should be mixed with different components in order to create composite materials. A good example is austenitic stainless steel (AA 316L), as a class of metallic materials used for biomedical applications. The aim of this chapter is to show a series of our group studies for obtaining a hybrid metalceramic composite by powder metallurgy, the physicochemical, mechanical, and biological properties of these hybrid composites and the investigation of possible functional coating layers and adherent bioactive hydroxyapatite Ca 10 (PO 4 ) 6 (OH) 2 on the metal of 316L stainless steel, correlating our data with literature data. Hardness properties of the obtained composites are close to those of human bones. In biological systems, could be observed that after 6 months, the relevant metals (Fe, Mn, Cr, and Ni) concentration (ppb), that may be released from composite materials in simulated physiological fluids (SBF), is practically zero.

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Hydroxyapatite coating on stainless steel pre-coated with bovine serum albumin at ambient conditions

Cited by 7 publications

References 2 publications

Nonfouling Property of Zwitterionic Cysteine Surface

Nonfouling Property of Zwitterionic Cysteine Surface

Influence of spray parameters on the microstructure and mechanical properties of gas-tunnel plasma sprayed hydroxyapatite coatings

316L Stainless Steel/Hydroxyapatite Composite Materials for Biomedical Applications

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