Hydroxyapatite microcarriers for biocontrolled release of protein drugs

Ijntema, K.; Heuvelsland, W. J. M.; Dirix, C.A.M.C.; Sam, Abbas

doi:10.1016/0378-5173(94)90357-3

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…However, this method has always been associated with a high ratio of initial drug release rather than constant release since drug release is based on diffusion or concentration gradient. [16][17][18] In that research, HAP served as an insulin carrier; however, instead of loading insulin on the surface of HAP, it can be loaded into the lattice spaces of HAP during its synthesis. [19][20][21][22] Aer insulin-loaded HAP (insHAP) is prepared, it can be injected by the IM route.…”

Section: Introductionmentioning

confidence: 99%

Insulin-loaded hydroxyapatite combined with macrophage activity to deliver insulin for diabetes mellitus

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Insulin-loaded hydroxyapatite combined with macrophage activity to deliver insulin for diabetes mellitus

et al. 2015

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“…Jntema et al employed HA microcrystals as microcarriers to load bovine serum albumin (BSA) of 5–10 wt.% and concluded that these can be used for biomedical applications such as drug delivery, orthopedics, and dentistry. [6] Matsumoto et al investigated the influence of protein concentration and crystallinity of HA particles on protein release from the HA nano-carrier. [7] In all of these cases, the biomolecules were adsorbed on the surface of HA carrier, however, burst release kinetics of protein was observed.…”

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confidence: 99%

Zn- and Mg-Doped Hydroxyapatite Nanoparticles for Controlled Release of Protein

et al. 2010

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Bovine serum albumin (BSA) protein incorporated with hydroxyapatite (HA) nanoparticles (NPs) were synthesized by in situ precipitation process. 2 mol% Zn 2+ and Mg 2+ were used as dopants to synthesize Zn 2+ /Mg 2+ doped HA-BSA NPs by in situ synthesis route. In our study we used BSA as a model protein. The amount of BSA uptake by doped and undoped HA NPs and subsequent release of BSA from NPs were investigated. Zn doped HA NPs showed the highest amount of BSA uptake, whereas the amount of BSA loaded in undoped HA NPs was the lowest. A two-stage BSA release profile from doped and undoped HA NPs was observed in phosphate buffer solution (PBS) at pH 7.2 ± 0.2. Initial burst release was due to the desorption of BSA from the HA surface. The later stage of slow release was controlled by the dissolution of BSA incorporated HA NPs. BSA release rate from Zn doped HA NPs was found to be the highest, whereas undoped HA NPs released BSA at the slowest rate. Our study showed that the protein release rate from HA NPs can be controlled by the addition of suitable dopants and doped HA based NP systems can be used in bone growth factor and drug release study. KeywordsNanoparticles; Hydroxyapatite; Dopant; Bovine serum albumin; Protein release IntroductionIn the recent years there has been increasing interest in inorganic nanoparticles (NPs) as carriers for macromolecules such as proteins, vaccines, and drugs. Numerous studies have shown that NPs can not only improve the resistance of therapeutic agents against enzymatic degradation, but also provide the possibility of transporting biomolecules to specific tissues, cells, and cell compartments in a controlled manner with a minimal invasive procedure. [1,3] Inorganic NPs have some potential advantages over other polymeric nanoparticulate based carrier systems, because of their low susceptibility to immune response as compared to viral vectors, low toxicity as opposed to organic NPs, and resistance to lipases and bile salts unlike liposomes.Among the inorganic NPs, hydroxyapatite (HA) has attracted much attention as a carrier for biomolecules because of its excellent biocompatibility and bioactivity. For orthopedic applications, porous HA-based implants infiltrated with bioactive agents or drugs have been reported. [4][5] However, limited surface area and unpredictable bioresorbability of HA implant have been the issues that remain to be resolved for the development of a controlled drug carrier system. In the past decade, these problems have been addressed in some research efforts directed towards the synthesis of HA micro or nano-carriers delivering antibiotics and growth factors with controlled release kinetics. Jntema et al. employed HA microcrystals as microcarriers to load bovine serum albumin (BSA) of 5-10 wt.% and concluded that these can NIH Public Access After washing, all the powders were dried at room temperature and stored in a freezer at −10°C.All the supernatants after every washing were collected and analyzed for concentration of BSA using a BCA protein assa...

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