Hydroxyapatite nucleation and growth mechanism on electrospun fibers functionalized with different chemical groups and their combinations

Cui, Wenguo; Li, Xiaohong; Xie, Cheng; Zhuang, Huihui; Zhou, Shaobing; Weng, Jie

doi:10.1016/j.biomaterials.2010.02.050

Cited by 159 publications

(146 citation statements)

References 33 publications

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“…Correspondingly, morphology of the tissue block on the non-porous PDLLA fiber mats showed no remarkable change from day three to day seven. This indicates that the nHA particles on the fibers surface of PDLLA/nHA composite scaffold could promote the adhesion and proliferation of chondrocytes from rabbits [44]. Immunofluorescence combined with CLSM observation further showed that both PDLLA and PDLLA/nHA composite fibrous scaffolds could support chondrocytes adhesion and proliferation ( Figure 10).…”

Section: Water Contact Angles Of Pdlla/nha Composite Fibrous Membranesmentioning

confidence: 81%

“…Correspondingly, morphology of the tissue block on the non-porous PDLLA fiber mats showed no remarkable change from day three to day seven. This indicates that the nHA particles on the fibers surface of PDLLA/nHA composite scaffold could promote the adhesion and proliferation of chondrocytes from rabbits [44]. The presence of nHA particles in composite fibrous membranes reduces the modulus and tensile strength, because the nHA particles rendered the electrospun fiber matrix stiffer and less plastic in its deformation, in a manner which was typical of hard inorganic phases [41,42].…”

Section: Water Contact Angles Of Pdlla/nha Composite Fibrous Membranesmentioning

confidence: 99%

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Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Qiang

Zhang²,

Feng³

et al. 2018

Applied Sciences

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Featured Application: The PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.Abstract: Porous poly-D, L-lactic acid (PDLLA) electrospinning fiber membrane was prepared, and nano-hydroxyapatite (nHA) was adsorbed and wrapped into it during the unique shrinking process of the PDLLA fiber membrane to fabricate the PDLLA/nHA composite membrane scaffold for tissue engineering. Compare with the composite fibers prepared by blend electrospinning, most of nHA particles are observed to distribute on the surface of new type composite fibers, which could significantly improve the water wettability and induce the cellular adherence. FTIR analysis indicated that the PDLLA/nHA composite fibrous membrane was formed by physical adsorption. The combination was probed by scanning electron microscope, thermo-gravimetric, water contact angle and mechanical property analysis. It was proved that the nHA particles' content and distribution, surface wettability, modulus and tensile strength of PDLLA/nHA composite fibrous membrane were influenced by the concentration of nHA dispersion and pores on the PDLLA fiber surface. The 10.6 wt % PDLLA/nHA composite fibrous membrane exhibits a more balanced tensile strength (3.28 MPa) and surface wettability (with a water contact angle of 0 • ) of the composite mats. Scanning electron microscope and confocal laser scanning microscopy images of chondrocyte proliferation further showed that the composite scaffold is non-toxic. The adherence and proliferation of chondrocytes on the 10.6 wt % PDLLA/nHA fibrous membrane was significantly improved, compared with PDLLA mat. The 10.6 wt % PDLLA/nHA composite fibrous membrane has potential application value as scaffold material in tissue engineering.

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Section: Water Contact Angles Of Pdlla/nha Composite Fibrous Membranesmentioning

confidence: 81%

“…Correspondingly, morphology of the tissue block on the non-porous PDLLA fiber mats showed no remarkable change from day three to day seven. This indicates that the nHA particles on the fibers surface of PDLLA/nHA composite scaffold could promote the adhesion and proliferation of chondrocytes from rabbits [44]. The presence of nHA particles in composite fibrous membranes reduces the modulus and tensile strength, because the nHA particles rendered the electrospun fiber matrix stiffer and less plastic in its deformation, in a manner which was typical of hard inorganic phases [41,42].…”

Section: Water Contact Angles Of Pdlla/nha Composite Fibrous Membranesmentioning

confidence: 99%

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Qiang

Zhang²,

Feng³

et al. 2018

Applied Sciences

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“…and PO 4 3-ions. Additionally, the resulting hydroxyl released from the PLA braids reacted with CPC as previously proposed [26,27,30]. The porous scaffolds produced by the incorporated bioceramics cannot be adequately applied clinically as bone substitute because of their native brittleness [31,32].…”

Section: Discussionmentioning

confidence: 95%

Comparison and preparation of multilayered polylactic acid fabric strengthen calcium phosphate-based bone substitutes for orthopedic applications

et al. 2015

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An attempt to maintain the three-dimensional space into restorative sites through the conveniently pack porous fillers are general used strategy. Advancement in the manufacturing protective shells in the scaffolds, which would be filled with brittle ceramic grafts for the development of highly connective pores provides the approach to solve crack problem for generating the tissues. Therefore, multilayered braided and alkalized poly(lactic acid) (PLA) composites with calcium phosphate bone cement (CPC) were synthesized and compared. The PLA/CPC composites were divided into various groups according to a series of heat-treatment temperatures (100-190 °C) and periods (1-3 h) and then characterized. The effects of 24-h immersion on the strength decay resistance of the samples were compared. Results showed that the residual oil capped on the surfaces of alkalized PLA braid was removed, and the structure was unaltered. However, the reduced tensile stress of alkalized PLA braids was due to ester-group formation by hydrolysis. Mechanical test results of PLA/CPC composites showed that the strength significantly increased after heat treatment, except when the heating temperature was higher than the PLA melting point at approximately 160-170 °C. The degree of PLA after recrystallization became higher than that of unheated composites, thereby leading to reduced strength and toughness of the specimen. Braiding fibers of biodegradable PLA reinforced and toughened the structure particularly of the extra-brittle material of thin-sheet CPC after implantation.

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“…23 The concentration, expressed as moles per mg of APLLA sc sample, was determined by means of a calibration curve obtained using known concentrations of distilled TEPA.…”

Section: Preparation Of Plla Sc and Surface Aminolysis Reactionmentioning

confidence: 99%

Amino-functionalized poly(L-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development

Bonito¹,

Petrone²,

Casini³

et al. 2015

IJN

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Background Poly( l -lactide) (PLLA) is a biodegradable polymer currently used in many biomedical applications, including the production of resorbable surgical devices, porous scaffolds for tissue engineering, nanoparticles and microparticles for the controlled release of drugs or antigens. The surfaces of lamellar PLLA single crystals (PLLA sc ) were provided with amino groups by reaction with a multifunctional amine and used to adsorb an Escherichia coli -produced human papillomavirus (HPV)16-E7 protein to evaluate its possible use in antigen delivery for vaccine development. Methods PLLA single crystals were made to react with tetraethylenepentamine to obtain amino-functionalized PLLA single crystals (APLLA sc ). Pristine and amino-functionalized PLLA sc showed a two-dimensional microsized and one-dimensional nanosized lamellar morphology, with a lateral dimension of about 15–20 μm, a thickness of about 12 nm, and a surface specific area of about 130 m 2 /g. Both particles were characterized and loaded with HPV16-E7 before being administered to C57BL/6 mice for immunogenicity studies. The E7-specific humoral-mediated and cell-mediated immune response as well as tumor protective immunity were analyzed in mice challenged with TC-1 cancer cells. Results Pristine and amino-functionalized PLLA sc adsorbed similar amounts of E7 protein, but in protein-release experiments E7-PLLA sc released a higher amount of protein than E7-APLLA sc . When the complexes were dried for observation by scanning electron microscopy, both samples showed a compact layer, but E7-APLLA sc showed greater roughness than E7-PLLA sc . Immunization experiments in mice showed that E7-APLLA sc induced a stronger E7-specific immune response when compared with E7-PLLA sc . Immunoglobulin G isotyping and interferon gamma analysis suggested a mixed Th1/Th2 immune response in both E7-PLLA sc -immunized and E7-APLLA sc -immunized mice. However, only the mice receiving E7-APLLA sc were fully protected from TC-1 tumor growth after three doses of vaccine. Conclusion Our results show that APLLA single crystals improve the immunogenicity of HPV16-E7 and indicate that E7-APLLA sc could be used for development of an HPV16 therapeutic vaccine against HPV16-related tumors.

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Hydroxyapatite nucleation and growth mechanism on electrospun fibers functionalized with different chemical groups and their combinations

Cited by 159 publications

References 33 publications

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Electrospun Porous PDLLA Fiber Membrane Coated with nHA

Comparison and preparation of multilayered polylactic acid fabric strengthen calcium phosphate-based bone substitutes for orthopedic applications

Amino-functionalized poly(L-lactide) lamellar single crystals as a valuable substrate for delivery of HPV16-E7 tumor antigen in vaccine development

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