Hydroxyl radical production and oxidative damage induced by cadmium and naphthalene in liver of Carassius auratus

Shi, Huahong; Sui, Yunxia; Wang, Xiaorong; Luo, Yi; Ji, Liangliang

doi:10.1016/j.cca.2005.01.009

Cited by 107 publications

(81 citation statements)

References 27 publications

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“…XRE is usually found in the promoter region of xenobiotics-metabolizing battery genes, such as cytochrome P450 (Ryu et al, 1996), glutathione S-transferase (Pimental et al, 1993), and UDP-glucuronosyltransferase 1A1 (Yueh et al, 2003), as well as mammalian sod1 (Yoo et al, 1999a;Park and Rho, 2002). The identification of XRE in this study is also in agreement with previous observations on the differential modulation of sod1 in other fish species exposed to xenobiotic compounds (Pedrajas et al, 1998;Shi et al, 2005;Vega-López et al, 2007). However unlike murine sod1 (Yoo et al, 1999b), the hmsod1 did not possess a metal-responsive element (MRE) in its promoter region, although heavy metal exposure could stimulate sod1 transcription in this species as well (see Cho et al, 2009b).…”

Section: Characteristics Of the 5'-flanking Regulatory Regionsupporting

confidence: 92%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

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Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

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Section: Characteristics Of the 5'-flanking Regulatory Regionsupporting

confidence: 92%

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Lee¹,

Kim²,

Bang³

et al. 2011

Fisheries and aquatic sciences

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“…The xenobiotics, such as insecticides are known to cause oxidative stress through the generation of ROS and can alter the antioxidants or free oxygen radical scavenging enzyme systems in aquatic organisms (Livingstone, 2001). ROS can react with susceptible biological macromolecules and cause LPO, DNA damage and protein oxidation (Nordberg and Arner, 2001;Shi et al, 2005). Thus, oxidative DNA damage may be the most putative mechanism of genotoxicity of environmental chemicals.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Ali

Kumar

et al. 2014

Chemical Speciation & Bioavailability

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Dimethoate is one of the organophosphate insecticides widely used in agriculture throughout the world and is an inhibitor of cholinesterase in animals. The objective of the present study was to detect oxidative stress and DNA damage induced by dimethoate in the freshwater fish Channa punctatus (C. punctatus). The LC 50 -96 h value of technical grade dimethoate was estimated at 19.10 µg L -1 in a semi-static system and, on the basis of the LC 50 value, three concentrations were determined. The fish were exposed to these concentrations of dimethoate for 96 h and samplings were done at 24 and 96 h for assessment of oxidative stress and DNA damage. After exposure to dimethoate, the level of superoxide dismutase declined while lipid peroxidation, glutathione, induction of micronucleus and DNA damage were increased in C. punctatus as the concentration and exposure time increased. Thus our results suggest that dimethoate induces genotoxic effects which invariably accompanied and correlated with oxidative stress.

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“…13 The real-time formation of ROS in the serum was investigated by mixing the samples with PBN spin trapping. The EPR settings were 3503.73 G center field, 20.00 mW microwave power, 5 G modulation amplitude, 50 G sweep width, 1 × 10 5 gain, 81.92 ms time constant, 125.95 s sweep time, 5 scans per sample.…”

Section: Ex Vivo Ros Evaluationmentioning

confidence: 99%

Influence of glycemic control on some real-time biomarkers of free radical formation in type 2 diabetic patients: An EPR study

et al. 2017

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Hydroxyl radical production and oxidative damage induced by cadmium and naphthalene in liver of Carassius auratus

Cited by 107 publications

References 27 publications

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Characterization of Copper/Zinc-Superoxide Dismutase (Cu/Zn-SOD) Gene from an Endangered Freshwater Fish Species Hemibarbus mylodon (Teleostei; Cypriniformes)

Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch)

Influence of glycemic control on some real-time biomarkers of free radical formation in type 2 diabetic patients: An EPR study

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