Hydroxylation of Progesterone and 11-Desoxy-17-Hydroxycorticosterone by Aspergillus and Penicillium

Abstract

by more than 99.9 per cent. Brilliant green was more effective against influenza A virus than against vaccinia virus, whereas Igepon AP-78 was more effective against vaccinia virus than against influenza A virus. The limitations of the experimental method were discussed.

Confirmation of the relationships of Aspergillus egyptiacus and Emericella nidulans using progesterone transformation

“…A . repens (Dulaney et al 1955). Aspergillus nidulans was reported to transform 16-a-hydroxyprogesterone into 1 1-a-, 16-a-dihydroxyprogesterone (Fried et al 1955).…”

Effect of Concentration on the Microbiological Hydroxylation of Progesterone1

“…Although the standard time period (72 hr) for the A. ochraceus experiments was considerably longer than the fermentation periods used by Dulaney et al (1955) and Karow and Petsiavas (1956), it is of interest to note that very little of the 6(,,11a-dihydroxyprogesterone was formed in the high concentration experiments. When similar fermentations were conducted at low concentrations, 0.5 to 1.0 g per L, we found extensive formation of the dihydroxyprogesterone.…”

“…The microbiological oxidation of progesterone to give 1la-hydroxyprogesterone is one of the most important routes to cortisone (Fieser and Fieser, 1959). Although a number of organisms can be used for this purpose (Eppstein et al, 1956), the two most widely used are Rhizopus nigricans (Peterson et al, 1952) and Aspergillus ochraceus (Dulaney et al, 1955). It is of obvious importance and utility to conduct these hydroxylations using the highest possible concentrations of substrate.…”

Progesterone biotransformation by plant cell suspension cultures

“…The A4-pregnene-6,f,14a-diol-3,20dione (IV) metabolite is also readily obtained with C. lunata (28), Circinella sp. (21), and Trichothecium roseum (20), while the 6fl,11adiol (V) could be obtained from Aspergillus and Penicillium (6).…”