Hydroxyproline, a Serum Biomarker Candidate for Gastric Ulcer in Rats: A Comparison Study of Metabolic Analysis of Gastric Ulcer Models Induced by Ethanol, Stress, and Aspirin

Takeuchi, Kenichiro; Ohishi, Maki; Endo, Keiko; Suzumura, Ken-ichi; Naraoka, Hitoshi; Ohata, Takeji; Seki, Jiro; Miyamae, Yoichi; Honma, Masashi; Soga, Tomoyoshi

doi:10.4137/bmi.s15918

Cited by 16 publications

(14 citation statements)

References 28 publications

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“…Serum hydroxyproline content is an indicator of collagen metabolism or tissue degradation. Previous studies also confirmed that hydroxyproline is decreased during gastric ulceration induced by NSAIDs and showed the increased activity of the collagenase enzyme in gastric ulcer models [40]. 2oxobutyrate was increased in the tissue samples after ulcer induction.…”

Section: Discussionsupporting

confidence: 62%

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Koushki

Yekta

Amiri-Dashatan

et al. 2019

BMC Complement Altern Med

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Background: Gastric ulcer is one of the most prevalent diseases worldwide. In Iranian folk medicine, Achillea wilhelmsii (AW) is used as a treatment for gastric ulcer. Previous reports also mentioned Antiulcerogenic properties for this herbal plant. This study investigated the therapeutic effects of Achillea wilhelmsii C. Koch extract on indomethacin-induced gastric lesion in rats, from both proteomic and metabolomic perspectives. Methods: The rats were divided into 4 groups. Gastric ulceration was induced by a single dose of indomethacin (45 mg/kg) by oral gavage. An amount of 800 mg/kg of AW extract was administered orally. Serum and tissue samples were collected for further investigations. The metabolomic study was performed by 1 H-NMR CPMG spectrometry. Proteomic analysis was also executed by using two dimensional gel electrophoresis (2DE) followed by liquid chromatography coupled to tandem mass spectrometry (LC-ESI/MS/MS). Real time PCR was used to confirm some of the genes. Results: The macroscopic and microscopic investigations confirmed the effectiveness of the AW extract. There was a panel of metabolites which showed alteration during gastric lesion development. The levels of some of these metabolite reversed nearly to their control values after the administration of AW extract. There were also changes in the levels of some proteins including Alb, Fabp5, Hspb1, Tagln, Lgals7, Csta and Myl9 which were reversed after AW administration. Conclusions: Our findings suggested that Achillea wilhelmsii C. Koch extract could be a potential therapy to be used for indomethacin-induced gastric lesion treatment in the future. However, further investigations are needed to confirm the results.

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Section: Discussionsupporting

confidence: 62%

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Koushki

Yekta

Amiri-Dashatan

et al. 2019

BMC Complement Altern Med

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“…The decrease in proline and HYP concentrations reflects an increased collagenase activity following a decrease in the collagen level of the stomach tissue (Takeuchi et al, 2013). A previous study (Takeuchi et al, 2014) also reported that HYP is a serum biomarker, which can predict NSAID-induced gastric damage in rats. In the present study, HYP level in G16 was found to be lower than that in other groups after 3 h. Further, it was statistically lower than that in G0 af-ter 9 and 18 h or that in G2 after 18 h and was lower than in all other groups after 36 h. A decrease in HYP was found in G16 (50%) compared to that obtained in G0, whereas this decrease was less severe in the other groups.…”

Section: Discussionmentioning

confidence: 90%

“…It has been reported that these changes are due to the activation of collagenase by NSAID-induced mitochondrial depression and by lesions present in the stomach. There is a significant correlation between the changes in HYP levels in the stomach and serum (Takeuchi et al, 2014).…”

Section: Acta Veterinaria Hungarica 67 2019mentioning

confidence: 92%

“…There are classic/conventional and novel biomarkers to determine the gastrointestinal, renal, and cardiac injuries. The increasing concentration in trefoil factor 2 (TFF2), which is a peptide (Poulsen et al, 1999) and the decreasing concentration in hydroxyproline (HYP), which is an amino acid (Takeuchi et al, 2014), have been used to determine the gastrointestinal damage. TFF2 is expressed and secreted preferentially by gastric mucous neck cells (Zhang et al, 2016) and plays a key role in the maintenance of mucosal integrity (Kaise et al, 2013).…”

Section: Acta Veterinaria Hungarica 67 2019mentioning

confidence: 99%

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Changes in novel gastrointestinal and renal injury markers in the blood plasma of sheep following increasing intravenous doses of tolfenamic acid

Yıldız

Çorum

Atik

et al. 2019

Acta Veterinaria Hungarica

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The administration of high doses of non-steroidal anti-inflammatory drugs (NSAID), such as tolfenamic acid (TA), has undesirable effects on different organs. Some novel biomarkers have been reported that can determine the gastrointestinal and renal injury caused by a high dose of NSAIDs or other toxic substances. This study was aimed at determining the changes in gastrointestinal (TFF2 and HYP), renal (NGAL and KIM-1) and cardiac (cTn-I, CK-MB) injury markers after the use of increasing intravenous doses of TA in sheep. TA was administered intravenously to groups of six sheep each, at the dose levels of 0 (Group 0, i.e., G0), 2 (G2), 4 (G4), 8 (G8) and 16 (G16) mg/kg. The concentrations of the studied biomarkers were measured at 3, 9, 18 and 36 h after administration of TA. The TFF2 and NGAL concentrations in G16 were found to be significantly higher (P < 0.05) than in the other groups except for G8 at different sampling times. HYP concentration in G16 was observed to be significantly (P < 0.05) lower than that in all other groups at 36 h. KIM-1 level in G16 was significantly (P < 0.05) higher than in all other groups at different sampling times. An increase in the renal markers, KIM-1 and NGAL, in G8 was observed before any change in plasma creatinine and urea. The cardiac marker cTn-I in G16 was significantly (P < 0.05) higher than in other groups at different sampling times. The results showed that the novel biomarkers (HYP, TFF2, NGAL, and KIM-1) can be used to determine gastric and renal injury in sheep.

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“…Details of the remaining CE–MS‐based metabolomics applications of the past two years can be found in Table .…”

Section: Applicationsmentioning

confidence: 99%

CE–MS for metabolomics: Developments and applications in the period 2014–2016

2016

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CE–MS can be considered a useful analytical technique for the global profiling of (highly) polar and charged metabolites in various samples. Over the past few years, significant advancements have been made in CE–MS approaches for metabolomics studies. In this paper, which is a follow‐up of a previous review paper covering the years 2012–2014 (Electrophoresis 2015, 36, 212–224), recent CE–MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined. Attention will be paid to new CE–MS approaches for the profiling of anionic metabolites and the potential of SPE coupled to CE–MS is also demonstrated. Representative examples illustrate the applicability of CE–MS in the fields of biomedical, clinical, microbial, plant, and food metabolomics. A complete overview of recent CE–MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, general conclusions and perspectives are given.

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Hydroxyproline, a Serum Biomarker Candidate for Gastric Ulcer in Rats: A Comparison Study of Metabolic Analysis of Gastric Ulcer Models Induced by Ethanol, Stress, and Aspirin

Cited by 16 publications

References 28 publications

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Therapeutic effects of hydro-alcoholic extract of Achillea wilhelmsii C. Koch on indomethacin-induced gastric ulcer in rats: a proteomic and metabolomic approach

Changes in novel gastrointestinal and renal injury markers in the blood plasma of sheep following increasing intravenous doses of tolfenamic acid

CE–MS for metabolomics: Developments and applications in the period 2014–2016

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