Hydroxyproline-rich glycoprotein mRNA accumulation in maize root cells colonized by an arbuscular mycorrhizal fungus as revealed by in situ hybridization

Balestrini, Raffaella; Josè-Estanyol, Matilde; Puigdomènech, Pere; Bonfante, Paola

doi:10.1007/bf01282129

Cited by 44 publications

(30 citation statements)

References 22 publications

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“…During the later steps of fungal colonization, chitin is ' naked ' and amorphous in the thin branches of arbuscules and can act as a potential source of elicitors. This hypothesis is supported by in situ hybridization experiments which have located transcripts of defence-related genes mainly in arbuscule-containing cells (Harrison & Dixon, 1994 ;Blee & Anderson, 1996 ;Gianinazzi-Pearson et al, 1996 ;Balestrini et al, 1997).…”

Section: Chitin Synthases In Fungal Growth and Morphogenesissupporting

confidence: 61%

Expression of chitin synthase genes in the arbuscular mycorrhizal fungus Gigaspora margarita

Lanfranco¹,

Vallino²,

Bonfante³

1999

New Phytologist

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Genomic DNA from spores of the endomycorrhizal fungus Gigaspora margarita was amplified with two sets of degenerate primers designed on conserved regions of chitin synthase polypeptides. Three chitin synthase (chs) gene portions were identified : Gimchs1, Gimchs2 and Gimchs3, encoding one class II and two class IV chitin synthases, respectively. Gimchs1 and Gimchs3 transcripts were detected by reverse transcription polymerase chain reaction (RT-PCR) during root colonization, whereas none of three mRNAs was revealed during spore germination. The potential role of the genes expressed is discussed in the light of morphogenetic and signalling events.

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Section: Chitin Synthases In Fungal Growth and Morphogenesissupporting

confidence: 61%

Expression of chitin synthase genes in the arbuscular mycorrhizal fungus Gigaspora margarita

Lanfranco¹,

Vallino²,

Bonfante³

1999

New Phytologist

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“…A differential expression study provides insight into up-regulation of PM-HRGP transcripts, observed only in resistant cultivars of pearl millet during downy mildew infection Accumulation of HRGP takes place in maize during wounding ), arbuscular mycorrhizal symbiosis (Balestrini et al 1997) and in response to fungal elicitors and infection (Showalter et al 1985). This phenomenon was not observed during wounding in other monocots such as barley, oat, wheat or rice (Mazau and Esquerretugaye 1986;Li and McClure 1990;SommerKnudsen et al 1998).…”

Section: Actamentioning

confidence: 99%

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

et al. 2011

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A monoclonal antibody, JIM 20, derived against an extensin type of hydroxyproline-rich glycoprotein (HRGP) from pea, showed high affinity for HRGP in pearl millet [Pennisetum glaucum (L.) R. Br.]. Electrophoretic separation of Tris-SDS extracted proteins from suspension cells of pearl millet revealed a range of PM-HRGP polypeptides having a glycan epitope, which reacted with JIM 20. A high molecular mass band, probably an HRGP aggregate or polymer, and a few low molecular mass polypeptides were recognized by JIM 20 during Western blot analysis. Treatment of pearl millet suspension cells with hydrogen peroxide in the presence of an endogenous peroxidase resulted in insolubilization of HRGP polypeptides with molecular weights between 45 and 33 kDa. To investigate the gene coding for an extensin type of HRGP, a fosmid-based genomic library of pearl millet having a fourfold genome coverage was constructed. A partial sequence of 378 bp of an HRGP gene was obtained by PCR amplification of pearl millet DNA with a primer pair designed from the conserved regions of monocotyledon extensin type of HRGPs. Screening the genomic library using the homologous probe developed from the 378-bp PCR product resulted in the isolation of five fosmid clones. Restriction mapping of these fosmids resulted in an 11.8-kb region around an HRGP gene in pearl millet. The newly characterized gene, PM-HRGP, had all the characteristic features of a monocotyledon extensin type of HRGP. An intron at the 3 0 untranslated region of the gene was identified by cDNA cloning. Differential expression of the PM-HRGP gene was observed during compatible and incompatible interactions of pearl millet with the downy mildew pathogen Sclerospora graminicola (Sacc) Schroet. Induced expression of the gene was observed only in case of an incompatible interaction.

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“…Perubahan serangkaian respon fisiologis dan biokimia tanaman dan efek perlindungannya terhadap berbagai cekaman ditentukan oleh derajat/persentase kolonisasi FMA yang dipengaruhi oleh kesesuaian inang-FMA dan lingkungan serta adanya perbedaan morfologi kolonisasi terutama ada/tidaknya arbuskular dan tipe arbuskular (arbuskular spesifik). Peningkatan ketahanan tanaman setelah diinduksi mikoriza berkaitan dengan fase awal perkembangan struktur mikoriza di dalam sel akar tanaman inang yang diawali sejak penetrasi oleh apresorium hingga bentuknya struktur arbuskular (Balestrini et al, 1997). Peningkatan aktivitas enzim peroksidase berhubungan erat dengan perkembangan struktur kolonisasi mikoriza.…”

Section: Pendahuluanunclassified

“…Hasil penelitian Gianinazzi-Pearson & Gianinazzi (1995) (Balestrini et al, 1997;Blee & Anderson, 2000), metabolisme enzim phenylpropanoid (Volpin et al, 1994;Harrison & Dixon, 1993;, enzim yang terlibat dalam metabolisme ROS (reactive oxygen species) (Blee & Anderson, 2000) dan enzim hydrolase (Salzer & Boller, 2000). Ditemukan adanya peningkatan jumlah HRGP mRNA di dalam akar tanaman jagung dan buncis yang mengandung arbuskular (Blee & Anderson, 2000).…”

Section: Pendahuluanunclassified

Aktivitas Enzim Peroksidase Pisang Kepok Dengan Aplikasi Glomus Tipe 1

Suswati¹,

Indrawaty²,

Friardi³

2016

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Ripe banana peroxidase activities with Glomus type 1. Ripe banana is very susceptible to blood disease caused by Blood disease bacterium (BDB) and Fusarium wilt caused by Fusarium oxysporum f.sp. cubense. Arbuscular mycorrhizal fungi (AMF) Glomus tipe 1 increased resistance of ripe banana seedlings to both wilt diseases. The resistance mechanism related with the change of hydrolytic enzyme activities: peroxidase (PO), phenylalanin amonialyase (PAL) and polyphenoloksidase (PPO). The green house and laboratorium experiment were conducted to study the effect of different colonization time 4, 12, 24, 36, 48, 72, 96 h after application (haa) and control (without AMF) with 3 replicates. AMF fresh inoculants source is a mixture of sand planting medium that containing spores, hyphae and corn root colonized AMF. Ripe banana seedlings (60 days old) were inoculated with 50 g fresh AMF inoculants and incubated with the treatment. The results showed that the application of Glomus tipe 1 caused changes in the enzyme activity of peroxidase in the roots and leaves. Root peroxidase enzyme activity slightly increased 5.84% (0.326U) at the beginning of colonization (4 haa) while peroxidase enzyme activity in leaves sharply declined (85.83-87.37%).Key words: arbuscular mycorrhizal fungi, blood disease bacterium, Fusarium oxysporum f.sp. cubense, Glomus tipe 1, peroxidase enzyme activities, ripe banana ABSTRAKAktivitas enzim peroksidase pisang kepok dengan aplikasi Glomus tipe 1. Tanaman pisang kepok sangat rentan terhadap penyakit layu bakteri yang disebabkan oleh Blood Disease Bacterium (BDB) dan layu Fusarium oleh Fusarium oxysporum f.sp. cubense (Foc). Aplikasi Glomus tipe 1 mampu meningkatkan ketahanan tanaman pisang kepok terhadap kedua penyakit tersebut. Peningkatan ketahanan tanaman pisang tersebut berkaitan erat dengan perubahan aktivitas enzim hidrolitik seperti peroksidase (PO), phenylalanin amonialyase (PAL) dan polypeniloksidase (PPO). Tujuan penelitian adalah mengukur aktivitas enzim peroksidase dan kadar protein tanaman pisang kepok setelah aplikasi Glomus tipe 1. Sebanyak 50 g inokulan Glomus tipe 1 diaplikasi pada perakaran bibit pisang kepok umur 2 bulan setelah aklimatisasi. Kolonisasi mikoriza pada perakaran bibit pisang Kepok disesuaikan dengan perlakuan waktu kolonisasi yaitu 4, 12, 24, 36, 48, 72, 96 jam setelah aplikasi (jsa) dan kontrol (tanpa mikoriza). Parameter yang diamati adalah aktivitas enzim peroksidase dan kadar protein tanaman pisang kepok dengan metode Lowry. Hasil penelitian menunjukkan bahwa aplikasi Glomus tipe 1 menyebabkan perubahan aktivitas enzim peroksidase dalam akar dan daun. Aktivitas enzim peroksidase akar sedikit meningkat 5,84% (0,326U) pada awal kolonisasi (4 jsa) sementara aktivitas enzim peroksidase dalam daun mengalami penurunan yang tajam (85,83-87,37%) .

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Hydroxyproline-rich glycoprotein mRNA accumulation in maize root cells colonized by an arbuscular mycorrhizal fungus as revealed by in situ hybridization

Cited by 44 publications

References 22 publications

Expression of chitin synthase genes in the arbuscular mycorrhizal fungus Gigaspora margarita

Expression of chitin synthase genes in the arbuscular mycorrhizal fungus Gigaspora margarita

Characterization of a hydroxyproline-rich glycoprotein in pearl millet and its differential expression in response to the downy mildew pathogen Sclerospora graminicola

Aktivitas Enzim Peroksidase Pisang Kepok Dengan Aplikasi Glomus Tipe 1

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