Hydroxyzine and Cetirizine Interfere With the PENTINA Carbamazepine Assay but Not With the ADVIA CENTEUR Carbamazepine Assay

Dasgupta, Amitava; Tso, Gertie; Johnson, Myrtle; Chow, Loretta

doi:10.1097/ftd.0b013e3181c6b0dc

Cited by 8 publications

(5 citation statements)

References 12 publications

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“…8 Cetirizine was chosen because it was one of the main crossreactants of the previously used monoclonal anti-CBZ antibody, although CET is not structurally closely related to CBZ. 19,20 Here, only semi-quantitative statements can be made, because these results were only produced to simplify the choice of the right antibody. All antibodies (from supernatants) showed very low CR (<1%) against DiOH-CBZ and CET.…”

Section: Characterization Of Antibodies In Cell Culture Supernatants mentioning

confidence: 99%

“…18 Previously described CBZ immunoassays were performed with a monoclonal anti-CBZ antibody, which showed high cross-reactivity (CR) against CBZ metabolites and related compounds, but also to the antihistaminic drug cetirizine which is not structurally close to CBZ. 19,20 This leads to overestimations of CBZ levels in water samples, especially during the hay fever season, when the antihistamine is present in waters. To avoid this effect, a new, more selective monoclonal antibody against CBZ was desirable.…”

Section: Introductionmentioning

confidence: 99%

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Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

Oberleitner

Dahmen-Levison²,

Garbe

et al. 2016

Anal. Methods

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Section: Characterization Of Antibodies In Cell Culture Supernatants mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

Oberleitner

Dahmen-Levison²,

Garbe

et al. 2016

Anal. Methods

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“…Cetirizine was extracted from Zyrtec tablet (cetirizine hydrochloride 10 mg; Pfizer, New York, NY), as described by Parant et al (7). Standard solution of carbamazepine 10, 11-epoxide (1 mg/ml) was prepared in absolute ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…The cross-reactivity of carbamazepine 10, 11-epoxide with different immunoassays for carbamazepine may vary between 0 (Vitros) and 94% (Dade Dimension) (6). Hydroxyzine and cetirizine, which are widely prescribed antihistamine drugs, also known to cross-react with the Dade Dimension particle-enhanced turbidimetric inhibition immunoassay (PETINIA) carbamazepine assay (7,8). In this study, we report our findings.…”

mentioning

confidence: 85%

Analytical performance evaluation of ADVIA Chemistry Carbamazepine_2 assay: minimal cross‐reactivity with carbamazepine 10, 11‐epoxide and none with hydroxyzine or cetirizine

Dasgupta

Reyes

Davis

et al. 2010

Clinical Laboratory Analysis

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Carbamazepine is an anticonvulsant requiring routine therapeutic drug monitoring. Recently, Siemens Healthcare Diagnostic Division released a new carbamazepine assay: ADVIA Chemistry Carbamazepine_2 (Carbamazepine_2) for application on ADVIA analyzers. We evaluated the analytical performance of this assay as well as its potential cross-reactivities with carbamazepine 10, 11-epoxide, hydroxyzine, and cetirizine. The within-run and between-run precisions of the Carbamzepine-2 assay were <6% and limit of detection was 0.5 microg/ml using ADVIA 1800 analyzer. The assay was linear up to a carbamazepine concentration of 20.0 microg/ml. The new method compared well with a widely used carbamazepine EMIT 2000 assay on the Hitachi 917 analyzer. Using 75 patients' specimens (where carbamazepine concentrations varied from 0.5 to 21.7 microg/ml) and carbamazepine EMIT 2000 as the reference method (x-axis), we observed the following regression equation: y=1.04 x+0.32 (r=0.99). The new carbazepine_2 method was not affected by a hemoglobin concentration of 1,000 mg/dl, conjugated or unconjugated bilirubin concentration of 60 mg/dl, and triglyceride concentration of 1,000 mg/dl. In addition, this assay showed no cross-reactivity with hydroxyzine or cetirizine and demonstrated minimal cross-reactivity with carbamazepine 10, 11-epoxide. We conclude that the ADVIA Chemistry carbamazepine_2 assay has adequate precision and accuracy for routine therapeutic drug monitoring of carbamazepine in clinical laboratories.

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“…68 The ADVIA centaur carbamazepine assay is also free from interferences of hydroxyzine and cetirizine. 69 …”

Section: Interferences In Immunoassays For Other Drugsmentioning

confidence: 97%

Impact of Interferences Including Metabolite Crossreactivity on Therapeutic Drug Monitoring Results

Dasgupta¹

2012

Therapeutic Drug Monitoring

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Therapeutic drug monitoring is an integral part of services offered by toxicology laboratories because certain drugs require routine monitoring for dosage adjustment to achieve optimal therapeutic response and avoid adverse drug reactions. Immunoassays are widely used for therapeutic drug monitoring. However, immunoassays suffer from interferences from both exogenous and endogenous compounds including metabolites of the parent drug. Digoxin immunoassays are affected more commonly than any other immunoassays used for therapeutic drug monitoring. Digoxin immunoassays are affected by endogenous digoxin-like immunoreactive substances and exogenous compounds such as various drugs, certain herbal supplements, and Digibind. Carbamazepine is metabolized to carbamazepine 10, 11-epoxide, and the crossreactivity of this metabolite with carbamazepine immunoassay may vary from 0% to 94%. Immunoassays used for measuring concentrations of tricyclic antidepressants are affected by tricyclic antidepressant metabolites and by a number of other drugs. Immunoassays for immunosuppressants are also subjected to significant interferences from metabolites, and liquid chromatography combined with mass spectrometry or tandem mass spectrometry is recommended for therapeutic drug monitoring of immunosuppressants. However, liquid chromatography combined with mass spectrometry may also suffer from interferences, for example, due to ion suppression or from isobaric ions.

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Hydroxyzine and Cetirizine Interfere With the PENTINA Carbamazepine Assay but Not With the ADVIA CENTEUR Carbamazepine Assay

Cited by 8 publications

References 12 publications

Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

Improved strategies for selection and characterization of new monoclonal anti-carbamazepine antibodies during the screening process using feces and fluorescence polarization immunoassay

Analytical performance evaluation of ADVIA Chemistry Carbamazepine_2 assay: minimal cross‐reactivity with carbamazepine 10, 11‐epoxide and none with hydroxyzine or cetirizine

Impact of Interferences Including Metabolite Crossreactivity on Therapeutic Drug Monitoring Results

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