Hylan G-F 20 maintains cartilage integrity and decreases osteophyte formation in osteoarthritis through both anabolic and anti-catabolic mechanisms

Li, P.; Raitcheva, D.; Hawes, M.; Moran, N.; Yu, Xijie; Wang, F.; Matthews, G.

doi:10.1016/j.joca.2012.07.004

Cited by 53 publications

(29 citation statements)

References 47 publications

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“…Pro-Hyp also stimulates the production of hyaluronic acid by cultured synovial cells (12,28). Notably, hyaluronic acid exhibits anti-inflammatory action (29). Based on these findings, it is tempting to speculate that Pro-Hyp, which is derived from orally administered CP, enhances the production of hyaluronic acid and glycosaminoglycans by synovial cells and chondrocytes, thereby exhibiting anti-inflammatory and chondroprotective actions (as evidenced by the suppression of MMP-13 expression, type II collagen degradation and morphological changes) in vivo in our model.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

Isaka

Someya

Nakamura

et al. 2017

Experimental and Therapeutic Medicine

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Collagen is an extracellular matrix protein present in the skin, tendon, cartilage and bone. Collagen peptides (CP) are produced by the hydrolysis of gelatin (heat-denatured collagen) by proteases and are utilized as a component of nutraceuticals. The current study investigated the effect of CP on the articular cartilage of OA by evaluating the serum levels of biomarkers (CTX–II for type II collagen degradation and CPII for type II collagen synthesis), histopathological changes (Mankin score, based on the toluidine blue staining of proteoglycans), and immunohistochemical staining of matrix metalloproteinase (MMP)-13 and type II collagen, using a rat experimental osteoarthritis (OA) model. Anterior cruciate ligament transection (ACLT) was performed on the right knee joint to surgically induce OA. Animals were divided into four groups: Control group (Control), sham-operated group (Sham), ACLT group without collagen peptide (ACLT group) and ACLT group with oral administration of CP (CP group). ACLT induced histological damages and significantly increased the Mankin score (P<0.05). However, CP administration markedly suppressed the Mankin score, although this difference was not significant. In addition, serum CTX-II levels were significantly decreased in CP group compared with those in the ACLT group (P<0.05). By contrast, serum CPII levels did not differ significantly among the four groups. Moreover, immunohistochemical staining of type II collagen and MMP-13 (an important type II collagen-degrading enzyme) indicated that the amount of type II collagen increased, whereas the number of MMP-13 positive chondrocytes decreased in the CP group compared with ACLT group. These observations suggest that CP has the potential to exert chondroprotective action on OA by inhibiting MMP-13 expression and type II collagen degeneration.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

Isaka

Someya

Nakamura

et al. 2017

Experimental and Therapeutic Medicine

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“…It is reported that inflammation-induced PGE 2 and NO concentrations could be suppressed by hylan G-F 20 [42]. Hylan G-F 20 can also interact with membrane receptor CD44 and intracellular adhesion molecule-1 (ICAM-1) for modulating the inflammatory effects, including inhibiting the IL-induced matrix-degrading enzymes and production of proinflammatory mediator, and also preventing cell apoptosis [43]. Collectively, hylan G-F 20 could potentially be further used for BPH treatment.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model

et al. 2017

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Benign prostatic hyperplasia (BPH) is the most common urologic disease among elderly men. A well-established in vitro cell model is required to determine the therapeutic mechanism of BPH inflammation. In this study, we attempted to establish an immortalized human prostate stromal cell line by transfecting with HPV-16 E6/E7 and designated as ihPSC. No significant difference was found in fibroblast-like morphology between primary hPSC and ihPSC. The ihPSC possessed a significantly higher cell proliferation rate than primary hPSC. The prostate-specific markers and proteins including cytoskeleton (α-SMA and vimentin) and smooth muscle (calponin), especially the androgen receptor (AR) were also examined in ihPSC, almost identical to the primary hPSC. To create an in vitro model featuring chronic prostatic inflammation, ihPSC was stimulated with IFN-γ+IL-17 and then treated with the high molecular weight hyaluronic acid hylan G-F 20 as an alternative strategy for inhibiting BPH inflammation. Hylan G-F 20 could dose-dependently diminish the inflammation-induced proliferation in ihPSC. The enhanced expressions of inflammatory molecules including IL-1β, IL-6, IL-8, cyclooxygenase 2 (COX2), inducible nitrogen oxide synthase (iNOS), and Toll-like receptor 4 (TLR4) were all abolished by hylan G-F 20. For inflammatory signaling, hylan G-F 20 can also diminish the IFN-γ+IL-17-increased expression of iNOS and p65 in ihPSC. These findings suggest that ihPSC could provide a mechanism-based platform for investigating prostate inflammation. The hylan G-F 20 showed strong anti-inflammatory effects by decreasing inflammatory cytokines and signalings in the ihPSC, indicating its therapeutic potentials in BPH treatment in the future.

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“…Positive effects were also found in comparison with patients without treatment (Bjørnland et al, 2007;Møystad et al, 2008); and no differences were found with the short-term use of intraarticular corticosteroids (Guarda-Nardini et al, 2005;Manfredini et al, 2010). However, the use of corticosteroids can present some adverse effects such as progression of the disease and condylar resorption (Wenneberg et al, 1991;El-Hakim et al, 2005), whereas the application of EHA does not show any major adverse effects (Li et al, 2012;Grossmann et al, 2013). According to Uchôa de Rezende & Constantino de Campos, the minimum adverse effects described were related to the infiltration procedure, such as effusion, joint pain, burning and joint erythema, which are all temporary.…”

Section: Characteristics Of the Temporomandibular Jointmentioning

confidence: 99%

Role of Hyaluronic Acid in the Homeostasis and Therapeutics of Temporomandibular Joint Osteoarthritis

et al. 2017

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Morphol., 35(3):870-876, 2017. SUMMARY:The aim of this study was to perform a literature review regarding the role of hyaluronic acid (HA) in the homeostasis and therapeutics of temporomandibular joint (TMJ) osteoarthritis (OA). The TMJ has characteristics that give it special adaptation and recovery abilities, where HA plays a fundamental role in helping to maintain joint homeostasis, which is affected in pathological processes like OA. OA is a chronic degenerative multi-factor disease that can affect all the components of the synovial joints, causing degradation of the articular cartilage, extracellular matrix and breakage in the HA molecules. HA is a non-branched linear polysaccharide with viscosupplementation, anti-inflammatory, lubrication and pain relief effects; it also activates the intrinsic repair processes of the cartilage and normalizes the endogenous production of HA by the synoviocytes. In recent years, the therapeutic use of HA has shown evidence that supports its application in TMJ OA, improving viscosupplementation capacity, acting at the cellular and molecular levels, reducing various inflammatory mediators and improving the reparative characteristics. Its use has been studied in animal models and in humans. However, no consensus has been reached in terms of concentrations, dose, application frequency or molecular weight to be used.

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Hylan G-F 20 maintains cartilage integrity and decreases osteophyte formation in osteoarthritis through both anabolic and anti-catabolic mechanisms

Abstract: Hylan G-F 20 improved cartilage integrity and decreased osteophyte formation in the rabbit model of OA. Our results suggest that hylan G-F 20 may stimulate cartilage repair by increasing Col II, and inhibit IL-1β-mediated matrix degradation by decreasing MMPs.

Cited by 53 publications

References 47 publications

Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

Evaluation of the effect of oral administration of collagen peptides on an experimental rat osteoarthritis model

Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model

Role of Hyaluronic Acid in the Homeostasis and Therapeutics of Temporomandibular Joint Osteoarthritis

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