Hymenobacter caeli sp. nov., an airborne bacterium isolated from King George Island, Antarctica

Abstract

A rod-shaped and Gram-stain-negative bacterial strain 9AT, was isolated from an air sample collected at King George Island, maritime Antarctica. Phylogenetic analysis based on 16S rRNA gene sequence reveals that strain 9AT belongs to the genus Hymenobacter and shows the highest similarity to Hymenobacter coccineus CCM 8649T (96.8 %). The DNA G+C content based on the draft genome sequence is 64.9 mol%. Strain 9AT is strictly aerobic, psychrophilic, catalase-positive, oxidase-positive and non-motile. Growth is observed at 0–20 °C (optimum 10 °C), pH 6.0–8.0 (optimum pH 7.0), and in the absence of NaCl. The predominant menaquinone of strain 9AT is MK-7 and the major fatty acids comprise Summed Feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 25.2 %), iso-C15 : 0 (23.2 %), C16 : 1 ω5c (11.6 %), Summed Feature 4 (anteiso-C17 : 1 B/iso-C17 : 1 I) (9.6 %) and anteiso-C15 : 0 (9.6 %). The polar lipid profile consists of the major lipid phosphatidylethanolamine and moderate to minor amounts of phosphatidylserine, unidentified aminolipids, aminophospholipids, aminophosphoglycolipids, polar lipids lacking a functional group and an unidentified phospholipid and a glycolipid. In the polyamine pattern sym-homospermidine is predominant. On the basis of the results obtained, strain 9AT is proposed as a novel species of the genus Hymenobacter , for which the name Hymenobacter caeli sp. nov. is suggested. The type strain is 9AT (=CCM 8971T=LMG 32109T=DSM 111653T).

Hymenobacter terricola sp. nov., isolated from Antarctic soil

“…The genus Hymenobacter belongs to the family Hymenobacteraceae in the phylum Bacteroidetes [5], and the type species of the genus is Hymenobacter roseosalivarius . Currently, the genus comprises 90 species with validly published names (https://lpsn.dsmz.de/genus/hymenobacter), including the recently described species Hymenobacter caeli [6]. Members of the genus Hymenobacter are generally Gram-stain-negative, aerobic, non-motile, pink to red pigmented and rod-shaped bacteria, and have a relatively high G+C content in their genomic DNA (55–70 mol%) [7–10].…”

Paenibacillus farraposensis sp. nov., isolated from a root nodule of Arachis villosa

“…Extraction of genomic DNA was carried out with the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit as described by the manufacturer. Amplification of nearly full-length 16S rRNA gene fragment was performed as previously described [13]. The sequence was compared to other publicly available sequences of type strains with validly published prokaryotic names deposited in the GenBank database.…”

Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada

“…The genus Hymenobacter encompassed 100 validly published species on LPSN (https://lpsn.dsmz.de/genus/hymenobacter) at the time of writing (December 2022) [2]. One hundred type strains of the genus Hymenobacter have been isolated from various samples including air, freshwater, freshwater sediment, glaciers, lichen, marine sediment, plant, sand, seawater, and soil collected in tropical to polar regions, wastewater and meat, implying that members of the genus Hymenobacter are successful colonisers in a wide range of environmental conditions (Table S1) [1, 3–84]. Type strains of the genus Hymenobacter are characterized as aerobic, rod-shaped, red-pink or pink-pigmented, Gram-stain-negative, and heterotrophic [6, 13, 20, 30].…”

“…The bacterial strain PAMC 29467 T was isolated from freshwater of the pond in Cambridge bay, Canada during a study on the physiological characterization of culturable bacteria from Arctic freshwater. Out of the 100 type strains of the genus Hymenobacter, 22 strains were obtained from air [2], glacier [5], lichens [2], marine sediment [1], red snow [1], rocks [4], and soils [7] of Antarctica or Arctic [1,6,7,13,16,20,36,40,53,64,72,79]. Thirteen strains of the genus Hymenobacter have been isolated from freshwater samples collected in Republic of Korea and Taiwan.…”