Hymenobacter ginkgonis sp. nov., isolated from bark of Ginkgo biloba

Abstract

Strain HMF4947T, isolated from the bark of a ginkgo tree, was a pale-pink coloured, Gram-stain-negative, non-motile, strictly aerobic and rod-shaped bacterium. The isolate grew optimally on Reasoner's 2A agar at 30 °C, pH 7.0 and with 0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HMF4947T belonged to the genus Hymenobacter and was most closely related to Hymenobacter metalli A2-91T (96.9 % sequence similarity) and Hymenobacter pomorum 9-2-1-1T (96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain HMF4947T and Hymenobacter arizonensis DSM 17870T were 74.3 and 20.5 %, respectively. The major fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15 : 0 and C16 : 1 ω5c. The predominant isoprenoid quinone was menaquinone-7. The polar lipids comprised phosphatidylethanolamine, one unidentified aminoglycolipid, three unidentified aminophospholipids, one unidentified phospholipid, three unidentified aminolipids, two unidentified glycolipids and three unidentified polar lipids. The genomic DNA G+C content was 59.3 mol%. Thus, based on phylogenetic, phenotypic and chemotaxonomic data, strain HMF4947T represents a novel species of the genus Hymenobacter , for which the name Hymenobacter ginkgonis sp. nov. is proposed. The type strain of the species is strain HMF4947T (=KCTC 72780T=NBRC 114271T).

Arthrobacter cavernae sp. nov., a novel actinobacterium isolated from sediment of karst cave

“…The pH range for growth was determined (pH 5.0, 6.0, 7.0, 8.0, 9.0, 10.0) and using R2A media adjusted by appropriate buffering agents 0.1 M sodium acetate buffer (pH 5.0–6.0), 0.1 M phosphate buffer (pH 7.0 and 8.0) or 0.1 M sodium carbonate buffer (pH 9.0 and 10.0). The ability for growth under anaerobic conditions was tested in R2A medium with or without 0.1 % (w/v) NaNO 3 [36]. To investigate the tolerance to NaCl, R2A broth was prepared in which sodium chloride concentrations was omitted (0 %) or added at 0.5–5.0 % (w/v, at intervals of 0.5 %) final concentration.…”

Hymenobacter canadensis sp. nov., isolated from freshwater of the pond in Cambridge Bay, Canada

“…The genus Hymenobacter encompassed 100 validly published species on LPSN (https://lpsn.dsmz.de/genus/hymenobacter) at the time of writing (December 2022) [2]. One hundred type strains of the genus Hymenobacter have been isolated from various samples including air, freshwater, freshwater sediment, glaciers, lichen, marine sediment, plant, sand, seawater, and soil collected in tropical to polar regions, wastewater and meat, implying that members of the genus Hymenobacter are successful colonisers in a wide range of environmental conditions (Table S1) [1, 3–84]. Type strains of the genus Hymenobacter are characterized as aerobic, rod-shaped, red-pink or pink-pigmented, Gram-stain-negative, and heterotrophic [6, 13, 20, 30].…”

Hymenobacter endophyticus sp. nov., isolated from wheat leaf tissue

“…As a result, Hymenobacter species frequently exhibit resistance to UV, cold temperatures and heavy metals. So far, a few Hymenobacter species have been obtained from plant tissues, including the bark of Ginkgo biloba [21], dry spikelet clusters of grass Setaria viridis [22] and Antarctic lichen [23]. In our previous study, Hymenobacter was found to be one of the dominant taxa in the microbial community of wheat leaves [24].…”