Hymenocallis littoralis ameliorates inflammatory responses in LPS-stimulated RAW264.7 cells and HCl/EtOH-induced gastric mucosal injury via targeting the MAPK pathway

Zhang, Jianmei; Sayakoummane, Sousath; Kim, Soo Ah; Lee, Jong Sub; Choung, Eui Su; Kim, Eun Sil; Lee, Seung-Gyu; Yum, Jinwhoa; Lee, Byoung-Hee; Lee, Sarah; Kim, Ji Hye; Cho, Jae Youl

doi:10.1016/j.jep.2022.115400

Cited by 9 publications

(6 citation statements)

References 52 publications

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“…MAPK signaling pathways are closely related to the inflammatory response [ 8 , 9 , 19 , 26 , 27 ]. Therefore, we monitored the MAPK signaling pathways including ERK, JNK and p38 pathways by Western blot analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Besides NF-κB and Akt signaling pathways, MAPK, including ERK and two stress-activated protein kinase families, JNK and p38, are stimulated by TLR4 and play an important role in inflammatory response in macrophages [ 8 , 9 , 19 , 26 , 27 ]. Therefore, much interest had focused on the effects of PF on the phosphorylation of ERK, JNK and p38 in LPS-stimulated RAW 264.7 macrophages.…”

Section: Discussionmentioning

confidence: 99%

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Phenethylferulate as a natural inhibitor of inflammation in LPS-stimulated RAW 264.7 macrophages: focus on NF-κB, Akt and MAPK signaling pathways

Yan,

Wang,

Song

et al. 2023

BMC Complement Med Ther

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Background Notopterygii Rhizoma et Radix (NRR) is commonly used for the treatment of inflammation-linked diseases. Phenethylferulate (PF) is high content in NRR crude, but its anti-inflammatory effect remains unclear. Therefore, we aimed to investigate the anti-inflammatory properties of PF and its underlying molecular mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods The effect of PF on cell viability was measured by MTT assay. The anti-inflammatory properties of PF were studied by detecting the levels of inflammatory mediators and cytokines using enzyme-linked immunosorbent assay (ELISA). Furthermore, the anti-inflammatory mechanisms of PF were determined by Western blot analysis. Results PF was not cytotoxic to RAW 264.7 macrophages at the concentrations of below 48 μM. ELISA showed that PF conspicuously inhibited overproduction of prostaglandin E2 (PGE2), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6). Western blot analysis showed that PF remarkably suppressed overproduction of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2), the phosphorylation of inhibitor of NF-κB kinase α (IκB-α), protein kinase B (Akt), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinases (JNK) and p38, as well as the degradation and subsequent nuclear translocation of p65. Conclusions PF is a potent inhibitor of inflammation acting on nuclear factor kappa-B (NF-κB), Akt and mitogen-activated protein kinase (MAPK) signaling pathways in LPS-stimulated RAW 264.7 macrophages. This work provides evidence for the suitability of PF as a therapeutic candidate for the management of inflammatory-mediated immune disorders.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phenethylferulate as a natural inhibitor of inflammation in LPS-stimulated RAW 264.7 macrophages: focus on NF-κB, Akt and MAPK signaling pathways

Yan,

Wang,

Song

et al. 2023

BMC Complement Med Ther

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“…According to the study of Jianmei Zhang, Hymenocallis littoralis (Jacq.) Salisb, also known as Pancratium littorale Jacq, exerts eminently anti-inflammatory activities in Lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and in Hydrochloric acid (HCl)/Ethanol (EtOH)-induced gastritis mice models in vivo and these activities could be attributed to its modulatory effects on the MAPK signaling pathway [26] . One study showed that Berberine (BBR) can improve CAG by reducing the expression of inflammatory factors TNF-α and IL-1β, down-regulating the expression of Transforming Growth Factor-beta (TGF-β) axis-related signals such as TGF-β1, PI3K, p-Akt/Akt and improving the gastric tissue injury of CAG rats [27] .…”

Section: Resultsmentioning

confidence: 99%

Network Pharmacology-Based Systematic Analysis and Molecular Mechanisms of Banxia Xiexin Decoction for Chronic Atrophic Gastritis

Wu¹,

Xiao²,

Zhao³

2022

IJPS

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Banxia Xiexin decoction, a traditional herbal mixture, has been widely used for chronic atrophic gastritis in clinical practice in China. However, research on the bioactive components and underlying mechanisms of Banxia Xiexin decoction in chronic atrophic gastritis is still scarce. Components and corresponding targets of Banxia Xiexin decoction were collected from the traditional Chinese medicine systems pharmacology database and analysis platform. The chemical structures of each component were obtained from PubChem. Meanwhile, targets of chronic atrophic gastritis were collected from DrugBank and Online Mendelian Inheritance in Man. Then, the herb-compound-target-disease network and the protein-protein interaction network were constructed based on the data obtained above. Cytoscape network centrality analysis was utilized to filter hub genes and VarElect was used to analyze the relationship between genes and diseases. At last, Metascape was employed for systematic analysis of the potential targets of herbals against chronic atrophic gastritis and AutoDock was applied for molecular docking to verify the results. A total of 214 components were screened as active components and there were 21 shared targets between Banxia Xiexin decoction and chronic atrophic gastritis. Two function modules of Banxia Xiexin decoction were found in the protein-protein interaction network. Further systematic analysis of shared genes and function modules explained the potential mechanism of Banxia Xiexin decoction in the treatment of chronic atrophic gastritis; molecular docking has verified the interactions. Banxia Xiexin decoction could be employed for chronic atrophic gastritis through mechanisms including complex interactions between related components and targets, as predicted by network pharmacology and molecular docking. This work confirmed that Banxia Xiexin decoction could apply for the treatment of chronic atrophic gastritis and promoted the explanation of Banxia Xiexin decoction for chronic atrophic gastritis in the molecular mechanisms.

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“…40 In short, inhibition of either MAPK pathways reduces inflammatory response. 41 It has also been reported that the MAPK pathway may suppress the downstream NF-κB pathway. 42 NF-κB is a cytoplasmic transcription factor that is phosphorylated in the cytoplasm and then translocated to the nucleus.…”

Section: Discussionmentioning

confidence: 99%

“…are produced, leading to neutrophil infiltration and intensified gastric mucosal damage. 39,41,46,47 Persistent inflammation is a predisposing factor for gastric mucosal damage. Meanwhile, TNF-α can promote the secretion of IL-1β, IL-6 and other cytokines, triggering the inflammatory response.…”

Section: Food and Function Papermentioning

confidence: 99%

Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

et al. 2023

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Hymenocallis littoralis ameliorates inflammatory responses in LPS-stimulated RAW264.7 cells and HCl/EtOH-induced gastric mucosal injury via targeting the MAPK pathway

Cited by 9 publications

References 52 publications

Phenethylferulate as a natural inhibitor of inflammation in LPS-stimulated RAW 264.7 macrophages: focus on NF-κB, Akt and MAPK signaling pathways

Phenethylferulate as a natural inhibitor of inflammation in LPS-stimulated RAW 264.7 macrophages: focus on NF-κB, Akt and MAPK signaling pathways

Network Pharmacology-Based Systematic Analysis and Molecular Mechanisms of Banxia Xiexin Decoction for Chronic Atrophic Gastritis

Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

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