Hyperbaric oxygen effects towards SIRT1 level in Sprague dawley with endothelial dysfunction by high-cholesterol diet

Setianingsih, Herin; Soetjipto, Soetjipto; Sudiana, I Ketut; Suryokusumo, Muhammad Guritno

doi:10.15562/bmj.v7i2.1154

Cited by 3 publications

(5 citation statements)

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“…These adaptive proteins play a role in cell protection, particularly in the context of endothelial dysfunction, through a series of signaling events induced by increased ROS molecules resulting from HBO exposure. Antioxidants such as HO-1 and SIRT1 have been identified as cardioprotective proteins [7,8]. Hydrogen peroxide (H2O2) constitutes the majority of ROS generated during hyperbaric oxygen therapy (HBO) [3].…”

Section: Resultsmentioning

confidence: 99%

“…The administration of HBO should carefully consider the dosage and interval of administration, as the appropriate dosage is crucial. Excessive elevation of ROS molecules can impede adaptive capacity of the cells to counteract the excess free radicals, resulting in a state of oxidative stress that may have detrimental effects on cells [8]. The concept of hormesis, preconditioning hypoxia, and redox theories serve as the primary mechanisms underlying hyperbaric oxygen therapy (HBO).…”

Section: Resultsmentioning

confidence: 99%

“…This apparent hypoxic state activates the transcription factor hypoxiainducible factor 1 (HIF-1), which serves as a cellular stress response adaptive mechanism. As a result, the oxygen saturation increases to 5.39 at a pressure of 2.4 ATA [7]. When a person is in a pressurized room (hyperbaric chamber) and subjected to a pressure of up to 2.4 ATA, the partial pressure of oxygen (pO2) increases tenfold, leading to a tenfold rise in the oxygen concentration in the blood compared to the expected level.…”

Section: Hypoxiamentioning

confidence: 99%

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Untitled

2023

J. Med. Chem. Sci.

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Background:The utilization and advancement of hyperbaric science continue to evolve, encompassing the investigation of cardiovascular disease. Macrophage activation stimulates the expression of matrix metalloproteinases, specifically MMP-12, which play a crucial role in atherosclerosis by influencing vascular tissue. Methods: This study aimed to examine MMP-12 expression in cardiovascular disease induced by an atherogenic high-fat diet and explore the effects of hyperbaric oxygen therapy. Previous research has focused on the primary mechanism of hyperbaric oxygen therapy through the SIRT1 (Sirtuin) pathway, which addresses endothelial dysfunction in atherosclerosis treatment. However, the impact of HBO utilization on the treatment and prevention of cardiovascular disease remains unexplored. Results: Molecular analysis of MMP-12 was conducted using a rat model administered a high-fat diet, followed by HBO treatment. Endothelial dysfunction caused by the high-fat diet was identified as a marker of cardiovascular disease, assessed by endothelin-1 (ET-1) levels. The administration of hyperbaric oxygen (HBO) yielded significant improvements in atherosclerosis and heart disease. The results, with a pvalue of 0.05, were significant for both the treatment and prevention of atherosclerotic heart disease in the two groups used as models. Conclusion: Sprague Dawley rats subjected to hyperbaric oxygen therapy at a pressure of 2.4 ATA, three sessions of 30 minutes each, for ten consecutive days, demonstrated potential for application in cardiovascular disease prevention and treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Hypoxiamentioning

confidence: 99%

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Untitled

2023

J. Med. Chem. Sci.

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“…This also increases the solubility of oxygen so that it can reach the cells. When increased to 3 ATA, the oxygen diffusion capacity increases 4 times compared to the pressure of 1 ATA 9,10 This can save cells or tissue from hypoxia so that they can survive. When breathing normal air, the arterial oxygen pressure is approximately 100 mmHg, and the tissue oxygen pressure is approximately 55 mmHg.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Hyperbaric Oxygen Therapy (HBOT) on the Histopathological Lung Cells of Male White Wistar Rats Which are Intoxicated with Methanol

Pratiknya,

Risma R,

Bintoro

2024

Pharmacogn J.

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Methanol is a liquid that has the characteristics of being light, very easy to evaporate, colorless, flammable, toxic, and has a very distinctive odor like ethanol but lighter. The World Health Organization (WHO) globally states that 320,000 people die every year due to methanol poisoning in the age range of around 15 years to 29 years. Usually, symptoms of poisoning caused by methanol appear within 30 minutes to 2 hours. Based on the phenomenon that occurred, the author wanted to find out more about the effect of HBOT administration on the histopathological appearance of lung cells in mice intoxicated with methanol. This study used 30 male white wistar rat lung samples aged 2 to 3 months weighing around 150 grams to 250 grams, divided into three groups, namely the control group, treatment group I, and treatment group II. The control group was not given methanol treatment and hyperbaric oxygen therapy (HBOT). Treatment group I was given methanol for 14 days without hyperbaric oxygen therapy. Meanwhile, the treatment group was given methanol for 14 days and then given hyperbaric therapy for 10 days. The results show p<0.0006, which means the data is significant and there are differences between groups. The results of this study can be concluded that administering hyperbaric oxygen therapy to rats intoxicated with methanol had a statistically significant improvement effect on the alveolar membrane, alveolar lumen, and connections between the alveoli.

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“…Based on study results, it has been demonstrated that resveratrol exhibits the ability to counteract the phenomenon of apoptosis in SGR. Resveratrol functions by the activation of SIRT1, a member of the nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylase family, which is recognized for its role in the modulation of cellular lifespan 35 . The enhancement of the antioxidant response to mitochondrial failure is achieved by the upregulation of SIRT1, resulting in a reduction in the expression of Bax, a known inducer of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Intravitreal Resveratrol as Anti Apoptotic Agent Against Retinal Ganglion Cell Loss in Ischemic Reperfusion Injury

Prasetya,

Komaratih,

Sasono

et al. 2024

Pharmacogn J.

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The in vivo experimental with post-test-only study was conducted in Universitas Airlangga from July 2022-January 2023. Mice models were used as the research subject, which were then divided into 4 groups through simple random sampling. The total research subjects were 20 eyeballs of Rattus Norvegicus. The groups were coded group G0, G1, G2, and G3. G0 was a control group with no treatment and enucleated at day 0. G1 was given I-R injury and enucleated at day 0 (IR0). G2 was given I-R injury, injected with intravitreal phosphate-ABSTRACT Background: Glaucoma is an optic neuropathy caused by the apoptosis of retinal ganglion cells and results in progressive retinal ganglion cell injury. A decrease in intraocular pressure (IOP) is a modifiable risk factor for slowing the progression of the disease, and can be accomplished through medication, laser therapy, or surgery. Even though the intraocular pressure has decreased and attained normal levels, the injury to the retinal ganglion cells continues in some cases. It is believed that neuroprotective administration has a positive effect on preventing the loss of retinal ganglion cells. Methods: Bax and Caspase-3 expression were measured involving 20 eyeballs of Rattus Norvegicus by immunohistochemistry examination. I-R injury was developed by increasing intraocular pressure (IOP) through the intracameral balanced salt solution (BSS) injection, then lowered after 60 minutes. Samples were divided into 4 groups: control, no further injection group, phosphate-buffered saline (PBS)-injected group and resveratrol-injected group. Each group was enucleated at days 7, 0, 7, and 7, respectively. Data with a non-normal distribution were examined using the Kruskal-Wallis test, and if the outcome was significant, the Mann-Whitney test. Results:The highest mean Bax and Caspase-3 expression was found in PBS injected and enucleated at day 7 group (G2), 0.96±0.40 and 0.72 ± 0.30, respectively. When compared to PBS injection, the expression of Bax and Caspase-3 was lower in the resveratrol-injected group. Conclusion: Bax and Caspase-3 expressions were lower in the intravitreal injection of Resveratrol in the dose of 100 µM following the I-R injury group compared to the group without intravitreal Resveratrol injection.

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Hyperbaric oxygen effects towards SIRT1 level in Sprague dawley with endothelial dysfunction by high-cholesterol diet

Cited by 3 publications

References 0 publications

Untitled

Untitled

The Effect of Hyperbaric Oxygen Therapy (HBOT) on the Histopathological Lung Cells of Male White Wistar Rats Which are Intoxicated with Methanol

Intravitreal Resveratrol as Anti Apoptotic Agent Against Retinal Ganglion Cell Loss in Ischemic Reperfusion Injury

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