Hypericin- and mTHPC-mediated photodynamic therapy for the treatment of cariogenic bacteria

Lüthi, Martin P.; Gyenge, Emina Bešić; Engstrüm, Mathias; Bredell, Marius; Grätz, Klaus W.; Walt, Heinrich; Gmür, Rudolf; Maake, Caroline

doi:10.1016/j.mla.2009.07.004

Cited by 25 publications

(31 citation statements)

References 44 publications

(61 reference statements)

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“…As the results of the cited studies show long irradiation times and high total energy doses were used to achieve sufficient suppression of the bacterial species 35, 40, 41. In contrast to this, in the present study we achieved complete suppression after an irradiation time of just 33 seconds and a total energy density of only 100 J cm −2 .…”

Section: Discussioncontrasting

confidence: 81%

“…The antibacterial effect of aPDT using mTHPC has been observed by other study teams as well. Recently, Lüthi et al 40 showed that Foslipos can be successfully used to suppress the cariogenic species Streptococcus mutans and Streptococcus sobrinus . Engelhardt et al 35 studied the photodynamic efficacy of Fospeg, a similar liposomal mTHPC formulation, against Staphylococcus aureus .…”

Section: Discussionmentioning

confidence: 99%

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Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

et al. 2011

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

et al. 2011

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“…Staph. sobrinus can be inactivated by 2 log after 15‐min incubation with 5 × 10 −6 –2 × 10 −5 mol l −1 Hyp and 128 J cm −2 illumination dose (Lüthi et al. 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Staph. sobrinus can be inactivated by 2 log after 15-min incubation with 5 · 10 )6 -2 · 10 )5 mol l )1 Hyp and 128 J cm )2 illumination dose (Lüthi et al 2009). To the contrary, several Gram-negative bacteria (Shigella flexnery 1a, S. flexnery 2a and Escherichia coli K1 no.…”

Section: Discussionmentioning

confidence: 99%

Effective inactivation of food pathogens Listeria monocytogenes and Salmonella enterica by combined treatment of hypericin-based photosensitization and high power pulsed light

Kairyte

Lapinskas

Gudelis

et al. 2012

Journal of Applied Microbiology

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Aims: The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATCL3C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)‐based photosensitization and high power pulsed light (HPPL). Methods and Results: Cells were incubated with Hyp (1 × 10−5 or 1 × 10−7 mol l−1) in PBS and illuminated with a light λ = 585 nm. For the combined treatment, bacteria were, after photosensitization, exposed to 350 pulses of HPPL (UV light dose = 0·023 J cm−2). Fluorescence measurements were performed to evaluate optimal time for cell–Hyp interaction. Results indicate that Hyp tends to bind both Listeria and Salmonella. After photosensitization treatment, Listeria population was reduced 7 log, whereas Salmonella was inactivated just 1 log. Electron photomicrograps of Salmonella and Listeria confirmed that photosensitization induced total collapse of the Listeria cell wall, but not that of Salmonella. After combined photosensitization–HPPL treatment, the population of Listeria was diminished by 7 log and Salmonella by 6·7 log. Conclusions: Listeria can be effectively inactivated by Hyp‐based photosensitization (7 log), whereas Salmonella is more resistant to photosensitization and can be inactivated just by 1 log in vitro. Combined treatment of photosensitization and pulsed light inactivates effectively (6·7–7 log) both the Gram‐positive and the more resistant to photosensitization Gram‐negative bacteria. Significance and Impact of the Study: A new approach to combat Gram‐positive and Gram‐negative bacteria is proposed, combining photosensitization with high power pulsed light.

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“…Thus potentially there is a role for PDT in oral cavity antibacterial action. However, while the study showed very good results for a single bacterial species it appears that the really multispecies environment of the oral cavity presents a significant treatment problem (452). The use of Fospeg was studied as well.…”

Section: Antibacterial Pdtmentioning

confidence: 99%

Temoporfin (Foscan^®, 5,10,15,20‐Tetra(m‐hydroxyphenyl)chlorin)—A Second‐generation Photosensitizer^†,‡

Senge

Brandt

2011

Photochem & Photobiology

289

218

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Hypericin- and mTHPC-mediated photodynamic therapy for the treatment of cariogenic bacteria

Cited by 25 publications

References 44 publications

Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

Effective inactivation of food pathogens Listeria monocytogenes and Salmonella enterica by combined treatment of hypericin-based photosensitization and high power pulsed light

Temoporfin (Foscan^®, 5,10,15,20‐Tetra(m‐hydroxyphenyl)chlorin)—A Second‐generation Photosensitizer^†,‡

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Hypericin- and mTHPC-mediated photodynamic therapy for the treatment of cariogenic bacteria

Cited by 25 publications

References 44 publications

Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

Photodynamic suppression of Enterococcus faecalis using the photosensitizer mTHPC

Effective inactivation of food pathogens Listeria monocytogenes and Salmonella enterica by combined treatment of hypericin-based photosensitization and high power pulsed light

Temoporfin (Foscan®, 5,10,15,20‐Tetra(m‐hydroxyphenyl)chlorin)—A Second‐generation Photosensitizer†,‡

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Product

Resources

About

Temoporfin (Foscan^®, 5,10,15,20‐Tetra(m‐hydroxyphenyl)chlorin)—A Second‐generation Photosensitizer^†,‡