Hypericin-assisted photodynamic therapy against anaplastic thyroid cancer

Kim, Hye-Jin; Kim, Sung Won; Seok, Kwang Hyuk; Hwang, Chi Woo; Ahn, Jin‐Chul; Jin, Jun‐O; Kang, Hyun Wook

doi:10.1016/j.pdpdt.2018.08.008

Cited by 47 publications

(24 citation statements)

References 38 publications

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“…In addition, several studies evaluated the reactions of natural and synthetic materials to laser irradiation. For example, photothermal therapy and photodynamic therapy use thermal or chemical responses of the material to irradiation to promote treatment efficacy . Therefore, further investigations of various biocompatible materials with LLLT will be pursued to maximize biomodulation effects or to achieve more synergistic effects on the fibrosis process.…”

Section: Discussionmentioning

confidence: 99%

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

et al. 2019

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Background and Objectives Fibrosis is a highly prevalent disease, which is responsible for 45% of deaths through pathological effects in developed countries. Previous studies have reported that low‐level laser therapy (LLLT) can modulate fibrotic activity, but significant enhancement of therapeutic efficacy is still required for clinical translation. The aim of this study is to evaluate the feasible effect of LLLT combined with phloroglucinol (PHL) on the inhibition of fibrosis in vitro. Study Design/Materials and Methods NIH/3T3 murine embryonic fibroblasts cells were cultured and transforming growth factor‐β1 (TGF‐β1) was treated for transition of fibroblasts. After TGF‐β1 treatment, LLLT and PHL were used, respectively, and in combination to suppress fibrosis. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and BrdU assays were performed to estimate the cell viability and proliferation. To evaluate the expression of fibrotic markers, we used confocal immunofluorescence and western blot. Results When compared with respectively treated groups, the group with the combined treatment of LLLT and PHL significantly reduced cell viability and proliferation. Immunofluorescence staining showed that the combined group minimized more α‐smooth muscle actin (α‐SMA) and type I collagen than the other groups. Western blot analysis showed that the combined treatment had significant decreases in α‐SMA, TGF‐β1, and type I collagen. Conclusions PHL‐assisted LLLT may be an effective treatment to inhibit fibrosis due to its additive effects. The combined treatment has a potential to be an alternative treatment for fibrosis. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

et al. 2019

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“…Photodynamic treatment with orange light and the Ps hypericin was reported to successfully decrease tumor volume in the nude mouse xenograft model, and orange light laser (589 nm) alone did not cause significant skin injury [46]. Similarly, in this study, we checked the harmful effect of an orange LED at a wavelength of 590 nm in advance, as seen in Figure S3.…”

Section: Apdt Rescued Intestinal Permeability Dysfunction In C Elegansmentioning

confidence: 91%

“…Mouse and rat animal models are used as in vivo models for the treatment of bacterial infections and tumors to observe and compare the improvement resulting from PDT [46][47][48]. In this study, we used the model nematode C. elegans.…”

Section: Apdt Rescued Intestinal Permeability Dysfunction In C Elegansmentioning

confidence: 99%

Antimicrobial Biophotonic Treatment of Ampicillin-Resistant Pseudomonas aeruginosa with Hypericin and Ampicillin Cotreatment Followed by Orange Light

Alam

Park

et al. 2019

Pharmaceutics

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Bacterial antibiotic resistance is an alarming global issue that requires alternative antimicrobial methods to which there is no resistance. Antimicrobial photodynamic therapy (APDT) is a well-known method to combat this problem for many pathogens, especially Gram-positive bacteria and fungi. Hypericin and orange light APDT efficiently kill Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), and the yeast Candida albicans. Although Gram-positive bacteria and many fungi are readily killed with APDT, Gram-negative bacteria are difficult to kill due to their different cell wall structures. Pseudomonas aeruginosa is one of the most important opportunistic, life-threatening Gram-negative pathogens. However, it cannot be killed successfully by hypericin and orange light APDT. P. aeruginosa is ampicillin resistant, but we hypothesized that ampicillin could still damage the cell wall, which can promote photosensitizer uptake into Gram-negative cells. Using hypericin and ampicillin cotreatment followed by orange light, a significant reduction (3.4 log) in P. aeruginosa PAO1 was achieved. P. aeruginosa PAO1 inactivation and gut permeability improvement by APDT were successfully shown in a Caenorhabditis elegans model.

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“…This hydrotropic effect is most likely a result of interactions between the two relative rigid aromatic rings of DSCG and a delocalized charge on the surface of the hypericin molecule. Kubin, et al [84] prepared a non-covalently bound hypericin-polyvinylpyrrolidone (PVP) complex to enhance the water solubility of hypericin. The hypericin-PVP complex bound more than 1000 mg of hypericin in presence of 100 g PVP and the resulting complex was soluble in 1 L of pure water.…”

Section: Water Solubility Of Hypericinmentioning

confidence: 99%

Hypericin: Source, Determination, Separation, and Properties

Zhang

Gao

et al. 2020

Separation & Purification Reviews

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Hypericin is a naturally occurring compound synthesized by certain species of the genus Hypericum, with various pharmacological effects. It is used as a natural photosensitizing agent with great potential in photodynamic therapy. This review discusses the latest results about the biosynthetic pathways and chemical synthetic routes to obtain hypericin. Although many analysis methods can be used for the determination of hypericin purity, HPLC has become the method of choice due to its fast and sensitive analyses. The extraction and purification of hypericin are also described. Hypericin can be used as a photosensitizer due to a large and active π-electron conjugated system in its structure. Medical applications of hypericin are not easy due to several unsolved practical problems, which include hypericin phototoxicity, poor solubility in water, and extreme sensitivity to light, heat, and pH.

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Hypericin-assisted photodynamic therapy against anaplastic thyroid cancer

Cited by 47 publications

References 38 publications

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

Antimicrobial Biophotonic Treatment of Ampicillin-Resistant Pseudomonas aeruginosa with Hypericin and Ampicillin Cotreatment Followed by Orange Light

Hypericin: Source, Determination, Separation, and Properties

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