Hypericin content variation in Hypericum perforatum in Australia

Southwell, Ian A.; Campbell, Malcolm

doi:10.1016/0031-9422(91)83708-s

Cited by 72 publications

(33 citation statements)

References 8 publications

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“…Regenerated plants described by Č ellárová et al (1994) had similar gland density of 0.20-0.29 on leaves as non-transformed control in our study. Density of dark glands on the top leaves of plants from different Australian populations varied from 0.33 to 0.50 glands per mm 2 of leaf area (Southwell and Campbell 1991) and that of northwestern USA populations from 0.61 to 0.85 (Walker et al 2001). On the other hand, Cirak et al (2007) reported on several times higher gland density on leaves.…”

Section: Morphological and Biochemical Traits Of Plants Ex Vitromentioning

confidence: 99%

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

et al. 2008

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Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones.

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Section: Morphological and Biochemical Traits Of Plants Ex Vitromentioning

confidence: 99%

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

et al. 2008

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“…The chemical constituents are predominantly accumulated in the flowers.Seven groups of bioactive natural products have been identified from H.perforatum (Southwell & Campbell 1991;Bratner et al 1994,Cellarova et al1994. Table 1 gives a summary of the active ingredients of Hypericum perforatum.…”

Section: Active Ingredientsmentioning

confidence: 99%

St. John's Wort

Gupta

Möller

2003

European Archives of Psychiatry and Clinical Neuroscience

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This article reviews the historical background, active ingredients of St. John's Wort and the major double-blind placebo-controlled studies. Despite the two major failed clinical trials conducted in American Research Centers, most of the data reviewed support that hypericum extracts are more effective than placebo for the treatment of mild to moderate depressive illness. The authors examine the likely reasons for the failed studies and also describe drug interactions and the side effects of St. John's Wort. Those patients who are prescribed St. John's Wort should be closely monitored.

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“…Callus tissues were induced from stem explants, which were transferred onto MS medium supplemented with 0.57 μM indole-3-acetic acid (IAA) and 2.22 μM 6-benzylaminopurine (BA). Hypericin was extracted from approximately 0.1 g of fresh tissues, as described by Southwell and Campbell (1991). In brief, ground plant tissues were extracted with 10 cm 3 of diethylether and re-extracted with 10 cm 3 of EtOH after all diethylether was evaporated.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum

Jin¹,

Ahn²,

Hwang³

et al. 2010

Biologia plant.

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Hypericin, a naphthodianthrone, has been identified as the principal active compound found in St. John's wort (Hypericum perforatum L.). To generate a gene resource for hypericin and other valuable metabolites, we generated expressed sequence tags (ESTs) from H. erectum. Analyses of the ESTs enabled us to select three cDNAs, HeHyp1, HeHyp2, and HeHyp3, evidencing significant sequence homology to Hyp-1 that were involved in hypericin biosynthesis from H. erectum. The deduced amino acid sequence of HeHyp1 cDNA exhibits 95 % identity with Hyp-1. The HeHyp2 and HeHyp3 polypeptides also exhibit 81.1 % identity with Hyp-1. The transcripts of HeHyp1, HeHyp2, and HeHyp3 were detected in the root, stem, leaf, flower, and callus cells. Study using recombinant protein suggests that Hyp-1, HeHyp2, and HeHyp3 may be involved in the biosynthetic of hypericin or other emodin derivatives.Additional key words: amino acid sequence, ESTs, HPLC, RT-PCR, TLC.

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Hypericin content variation in Hypericum perforatum in Australia

Cited by 72 publications

References 8 publications

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

Characterization of hairy root-phenotype in transgenic Hypericum perforatum L. clones

St. John's Wort

Isolation and functional analysis of cDNAs similar to Hyp-1 involved in hypericin biosynthesis from Hypericum erectum

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