Hypericin encapsulated in solid lipid nanoparticles: Phototoxicity and photodynamic efficiency

Photodynamic efficiency of hypericin compared with chlorin and hematoporphyrin derivatives in HEp-2 and Vero epithelial cell lines

“…A nonhuman cell line has fewer biohazard restrictions and has the advantage that the tissue from which it was derived may be more accessible [29]; nevertheless, PDT studies using cell lines of different origin species compared with human cell lines have been described previously [30,31]. The cells were grown as adherent monolayers in 25 cm 2 flasks with Iscove's modified Dulbeccos's medium supplemented with 10% FBS (Fetal Bovine Serum), penicillin (10,000 U.I.…”

Effects of continuous wave and fractionated diode laser on human fibroblast cancer and dermal normal cells by zinc phthalocyanine in photodynamic therapy: A comparative study

“…This medical treatment modality is based on the localization of a chemical photosensitizer in the target cells followed by light irradiation at certain wavelength that corresponds to the peak absorption of the photosensitizer into the target tissue. The light irradiation leads to the excitation of the photosensitizer to the triplet state from the singlet state through intersystem crossing and transferring the excited energy to ground state molecular oxygen generating cytotoxic reactive oxygen species (ROS) such as OH•, O 2 • − and excited singlet oxygen ( 1 O 2 ) that destroy target cells through apoptosis or necrosis [4,10,11].…”

Potentiation of hypericin-mediated photodynamic therapy cytotoxicity by MK-886: Focus on ABC transporters, GDF-15 and redox status

“…Combinations of multiple therapeutic modalities can provide a broader range of coverage against resistant tumour cells, and allows the use of individual drugs at lower doses, reducing toxic side-effects, as already proved in clinical practice. PDT efficacy can be potentiated through multiple mechanisms, including combination with antibiotics [37] and antioxidant inhibitors [38], conjugation with nanocarrier systems [39] and pretreatment with various AA metabolism inhibitors [40]. We already proved that MK-886 increases sensitivity of tumour cells to HY-PDT [19,20,40].…”