Hyperoside Ameliorates Diabetic Retinopathy via Anti-Oxidation, Inhibiting Cell Damage and Apoptosis Induced by High Glucose

Abstract

Background: Hyperoside (Hyp) is a flavonoid substance extracted from plants, which has the functions of anti-cancer, anti-inflammatory, and anti-oxidation. In the previous study, we found that Hyp reduced the injury of rat retinal vascular endothelial cells (RVECs) induced by H 2 O 2. Method: In the present research, we evaluated the protective effect of Hyp on the pathological damage of retina caused by high glucose of diabetes mellitus (DM) in in vitro and in vivo experiments. The effect of Hyp on cell viability, oxidative stress level, and apoptosis of RVECs was assessed. Results: Hyp significantly reduced the of RVECs damage, oxidative stress level, and cell apoptosis induced by high glucose in vitro. In DM model rats, Hyp treatment could significantly reduce blood glucose levels and the pathological damage of retina caused by DM and increase the proliferation of RVECs while exerting the inhibition on apoptotic activity. Furthermore, Hyp treatment decreased the expressions of apoptotic proteins including caspase-3, caspase-9, and Bax in RVECs of DM rats, while increased the expression of anti-apoptotic protein Bcl-2. Conclusion: Hyp may have protective effect on diabetes-induced retinopathy by reducing oxidative stress, inhibiting cell damage, and apoptosis induced by high glucose.

Chemical Characteristics and Antioxidant Activity of Arctostaphylos uva-ursi L. Spreng. at the Southern Border of the Geographical Range of the Species in Europe

“…and Crataegus spp. This metabolite shows broad-spectrum biological and therapeutic activities, e.g., antioxidant [53,54] anti-inflammatory [55], and anticancer effects [53]. The HYP concentrations in the extracts analyzed in this study are generally high; they are several times higher (in the case of the leaves collected in the heathlands) than the values (2.92 mg g −1 ) reported by Olennikov and Chekhirova [25].…”

Protective Role of St. John’s Wort and Its Components Hyperforin and Hypericin against Diabetes through Inhibition of Inflammatory Signaling: Evidence from In Vitro and In Vivo Studies

“…HPF and HYP are the characteristic compounds of SJW, being largely more abundant in this herbal species than in other plants, while for instance rutin, hyperoside, and quercetin are mostly present in a large variety of vegetables and fruits. Rutin, hyperoside, and quercetin have been reported to possess antioxidant, antitumoral, and also antidiabetic properties [80,[90][91][92][93], but their putative pharmacological use, as well as that of other flavonoids, is much limited by their scarce bioavailability, mainly due to poor intestinal absorption [91,94,95]. Furthermore, flavonoid physiological and pharmacological effects, including anti-inflammatory activity, are observed in vitro only at quite high doses (usually much more than 10 µM), hardly achievable in vivo [96,97].…”

Variation in Population and Solvents as Factors Determining the Chemical Composition and Antioxidant Potential of Arctostaphylos uva-ursi (L.) Spreng. Leaf Extracts

“…The hyperoside concentrations presented in this paper are significantly higher than those reported for heathland populations [ 10 ]. Hyperoside is an active compound found in plants of the genera Hypericum and Crataegus exhibiting antioxidant, anticancer, and anti-inflammatory activities [ 61 , 62 , 63 ].…”