Hyperpolarized 129Xe dynamic study in mouse lung under spontaneous respiration: Application to murine tumor B16BL6 melanoma

Imai, Hirohiko; Kimura, Atsuomi; Ito, Tetsuhide; Fujiwara, Hideaki

doi:10.1016/j.ejrad.2008.09.033

Cited by 15 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, xenon-129 in the dissolved phase experiences a significant chemical shift of its resonance frequency. As a result, the NMR spectrum of HXe129 in the lung shows several resonances: xenon in the gas phase (0 ppm), dissolved in tissue or blood plasma (∼198 ppm) and bound to hemoglobin (species dependent, 200-218 ppm) (11-14). With appropriately designed acquisition methods, it is therefore possible to distinguish gas- and dissolved-phase compartments in the lung: spectroscopy and chemical shift imaging directly quantify the xenon-129 magnetization in the respective compartments (11); in Dixon-type acquisitions compartment distinction is encoded via the magnetization phase (15, 16); direct dissolved-phase imaging was demonstrated recently in humans (17); and choosing appropriate imaging parameters in Cartesian gradient-echo pulse sequences allows simultaneous mapping of gas- and dissolved-phase xenon-129 (18).…”

Section: Introductionmentioning

confidence: 99%

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Dregely

Ruset

Mata

et al. 2011

Magnetic Resonance in Med

View full text Add to dashboard Cite

Hyperpolarized xenon-129 is a non-invasive contrast agent for lung MRI, which upon inhalation dissolves in parenchymal structures, thus mirroring the gas-exchange process for oxygen in the lung. Multiple-exchange-time Xenon polarization Transfer Contrast (MXTC) MRI is an implementation of the XTC MRI technique in four dimensions (3 spatial dimensions plus exchange time). The aim of this study was to evaluate the sensitivity of MXTC MRI for the detection of microstructural deformations of the healthy lung in response to gravity-induced tissue compression and the degree of lung inflation. MXTC MRI was performed in four rabbits and in three healthy human volunteers. Two lung function parameters, one related to tissue- to alveolar-volume ratio and the other to average septal-wall thickness, were determined regionally. A significant gradient in MXTC MRI parameters, consistent with gravity-induced lung tissue deformation in the supine imaging position, was found at low lung volumes. At high lung volumes, parameters were generally lower and the gradient in parameter values was less pronounced. Results show that MXTC MRI permits the quantification of subtle changes in healthy lung microstructure. Further, only structures participating in gas exchange are represented in MXTC MRI data, which potentially makes the technique especially sensitive to pathological changes in lung microstructure affecting gas exchange.

show abstract

Section: Introductionmentioning

confidence: 99%

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Dregely

Ruset

Mata

et al. 2011

Magnetic Resonance in Med

View full text Add to dashboard Cite

show abstract

“…14 Our previous in vivo study in mice also revealed a slight diŠerence in the dissolved-phase spectrum of HP 129 Xe from results reported in rats, canines, and humans. [15][16][17] We observed 3 dissolvedphase signals, at 192, 196, and 200 ppm, and conrmed that HP 129 Xe dissolved in mouse blood does not appear around 212 ppm. We believe a peak around 212 ppm has not been observed for mice.…”

Section: Introductionmentioning

confidence: 88%

Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

et al. 2011

Self Cite

View full text Add to dashboard Cite

Pulmonary study using hyperpolarized (HP) 129 Xe gas as an imaging medium must focus on dissolved-phase signals to make the most of the characteristic a‹nity of xenon for biological tissues, including blood. However, the spectral pattern of these signals diŠers between mice and other animals, including rats, canines, and humans. Dissolved-phase study has been reported only scarcely in mice, so spectral assignment has been an important subject for HP 129 Xe magnetic resonance (MR) spectroscopy (MRS) and MR imaging for its wider application. We performed MRS, including magnetization transfer experiments, and MR imaging studies to conˆrm the origin of dissolved-phase signals of mice ex vivo and in vivo and obtained evidence to assign dissolved-phase signals at 192 ppm for epicardial fat, 196 ppm for lung parenchyma, and 200 ppm for blood. These results were theˆrst to show the possibility of fast exchange of xenon between plasma and red blood cells.

show abstract

“…Xe was polarized by the optical pumping method with a home-built noble gas polarizer (18,19). All MRI measurements were performed with an Agilent Unity INOVA 400WB (Agilent Technologies, Inc., Santa Clara, CA).…”

Section: Micro-ctmentioning

confidence: 99%

Validation of Noninvasive Morphological and Diffusion Imaging in Mouse Emphysema by Micro–Computed Tomography and Hyperpolarized ¹²⁹Xe Magnetic Resonance Imaging

Tetsumoto¹,

Takeda²,

Imai

et al. 2013

Am J Respir Cell Mol Biol

Self Cite

View full text Add to dashboard Cite

Animal disease models are pivotal in investigating the pathogenesis of emphysema and developing novel drugs, but the modalities to evaluate murine emphysema models have been of limited validity and sensitivity. In this study, we evaluated hyperpolarized (129)Xe magnetic resonance imaging (MRI) and micro-computed tomography (micro-CT) compared with traditional methods, such as plethysmography and histology. Elastase-treated mice and adiponectin knockout mice were used as murine emphysema models to evaluate these modalities. Three weeks after elastase administration, significant and heterogeneous emphysema was evaluated according to the mean linear intercept and plethysmography parameters. Notably, the distribution of low-density areas, as examined by micro-CT, correlated with the mean linear intercept and plethysmography parameters in whole lungs. These correlations were also observed in regional areas. Furthermore, we introduced hyperpolarized (129)Xe MRI, which can evaluate gas exchange between the alveoli and blood during spontaneous breathing. Parameters of gas exchange (fD) and alveolar size (Vs/Va) were significantly decreased in elastase-treated mice, and moderately correlated with the plethysmography parameters. Of importance, we could detect a decrease of the fD value in low-density areas with micro-CT, suggesting that gas exchange decreased in emphysematous lesions. Likewise, these parameters (fD and Vs/Va) were also decreased in adiponectin knockout mice, which exhibit emphysema with a homogeneous distribution. We demonstrated the feasibility of (129)Xe MRI and micro-CT in combination with traditional modalities. These noninvasive modalities provide complementary data that can be used for repeated estimations of regional gas exchange and lung morphology.

show abstract

Hyperpolarized 129Xe dynamic study in mouse lung under spontaneous respiration: Application to murine tumor B16BL6 melanoma

Cited by 15 publications

References 44 publications

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

Validation of Noninvasive Morphological and Diffusion Imaging in Mouse Emphysema by Micro–Computed Tomography and Hyperpolarized ¹²⁹Xe Magnetic Resonance Imaging

Contact Info

Product

Resources

About

Hyperpolarized 129Xe dynamic study in mouse lung under spontaneous respiration: Application to murine tumor B16BL6 melanoma

Cited by 15 publications

References 44 publications

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Multiple‐exchange‐time xenon polarization transfer contrast (MXTC) MRI: Initial results in animals and healthy volunteers

Origin of Dissolved-phase Hyperpolarized 129Xe Signal in the Mouse Chest Based on Experimental Evidence from Extensive Magnetic Resonance Measurements

Validation of Noninvasive Morphological and Diffusion Imaging in Mouse Emphysema by Micro–Computed Tomography and Hyperpolarized 129Xe Magnetic Resonance Imaging

Contact Info

Product

Resources

About

Validation of Noninvasive Morphological and Diffusion Imaging in Mouse Emphysema by Micro–Computed Tomography and Hyperpolarized ¹²⁹Xe Magnetic Resonance Imaging