Hyperproduction of phycobiliproteins by the cyanobacterium Anabaena fertilissima PUPCCC 410.5 under optimized culture conditions

Khattar, J. I. S.; Kaur, Sandeep; Kaushal, Shveta; Singh, Yadvinder; Singh, Devinder; Rana, Shiwali; Gulati, Arvind

doi:10.1016/j.algal.2015.10.007

Cited by 37 publications

(36 citation statements)

References 52 publications

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“…Phycobiliproteins (PBPs) are water‐soluble chromoproteins may account up to 60% of the cellular proteins of cyanobacteria which have an accessory role in photosynthesis by the capture of different wavelengths of light (Khattar et al . ). Cellular proteins are important targets of UV radiation due to the presence of aromatic amino acids with absorption maxima in the UV region.…”

Section: Discussionmentioning

confidence: 97%

“…Periodic fluctuations of PBPs have also been observed by Khattar et al . in which PAR exposure promotes the synthesis of PE and PC and UV‐B radiation causes a reduction in total PBPs during light phase and induction during the dark phase. Similarly, a circadian rhythm of degradation and replacement of damaged pigments was detected in L .…”

Section: Discussionmentioning

confidence: 99%

“…One of the protective mechanisms in cyanobacteria permit them to efficiently absorb light energy without producing reactive oxygen species (ROS) is chromatic adaptation due to changes in the content and ratio of phycobilisomes (Bhandari & Sharma 2006). Phycobiliproteins (PBPs) are water-soluble chromoproteins may account up to 60% of the cellular proteins of cyanobacteria which have an accessory role in photosynthesis by the capture of different wavelengths of light (Khattar et al 2015). Cellular proteins are important targets of UV radiation due to the presence of aromatic amino acids with absorption maxima in the UV region.…”

Section: Discussionmentioning

confidence: 99%

“…C: Concentration A: Absorbance ε: extinction coefficients To determine phycobiliprotein content, extraction of dried mass from each UV-treatment was done using sodium phosphate buffer of 0.05 M, pH 7. The mixture was subjected to freeze/thaw and sonication treatment in order to break the cell walls and effectively extract the water-soluble phycobiliproteins (PBPs) into aqueous media (Eriksen 2008;Moraes et al 2011;Khattar et al 2015). Then, the samples were incubated overnight at 4 C. After extraction, the cell debris was removed by centrifugation at 10 000 rpm for 15 min, 4 C. The contents of phycobiliproteins were measured by reading the supernatant at 562, 615, and 652 nm using a UV-visible spectrophotometer according to following equations (Sudhakar et al 2015).…”

Section: = A=εmentioning

confidence: 99%

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Effects of different UV radiation on photoprotective pigments and antioxidant activity of the hot‐spring cyanobacterium Leptolyngbya cf. fragilis

Kokabi

Yousefzadi

Soltani

et al. 2019

Phycological Research

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SUMMARY UV‐induced synthesis/accumulation of photoprotective pigments and antioxidant activity were investigated in the hot‐spring cyanobacterium Leptolyngbya cf. fragilis. The results indicated that UV radiation may induce biosynthesis of carotenoids, allophycocyanin, phycoerythrin, and scytonemin while phycocyanin degrades in response to longtime UV radiation. Moreover, pigment composition of L. cf. fragilis was significantly altered with increasing UV radiation times, probably due to destruction and resynthesis of accessory pigments as an adaptation strategy to UV stress. The in vitro antioxidant analysis of different extracts of UV treated cyanobacteria exhibited concentration‐dependent antioxidant activity. Ethyl acetate extract of 72 h UV treatment showed maximum total antioxidant activity (IC50 = 71.73 ± 5.3 μg mL−1) followed by ethyl acetate control (non‐UV irradiated) extract (IC50 = 109.43 ± 2.76 μg mL−1). This is the first report for the UV‐induced synthesis of photoprotective pigments and their antioxidant activity in L. cf. fragilis.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: = A=εmentioning

confidence: 99%

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Effects of different UV radiation on photoprotective pigments and antioxidant activity of the hot‐spring cyanobacterium Leptolyngbya cf. fragilis

Kokabi

Yousefzadi

Soltani

et al. 2019

Phycological Research

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“…In addition to the panoply of ecosystem services that marine microalgae and macroalgae provide, the extensive range of biotechnological exploitation and the subsequent industrial applications of these organisms as biological factories are thoroughly documented. Marine algae are being widely used ( Figure 6)-both at the molecular and organismal levels-as food [85,86] and nutraceuticals [87], animal and fish feed [86,88], biofertiliser [89], bioplastics [90], pharmaceuticals [91], cosmeceuticals [91][92][93], fluorophores [94], food colourants and textile dyes [95,96], and biofuels [16] as well as for phycoremediation [97][98][99] ( Table 1).…”

Section: Existing and Potential Applications Of Marine Algaementioning

confidence: 99%

Challenges and Opportunities in the Present Era of Marine Algal Applications

Beetul¹,

Gopeechund²,

Kaullysing³

et al. 2016

Algae - Organisms for Imminent Biotechnology

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Marine algae are of high importance in their natural habitats and even more now in the world of green technology. The sprouting interest of the scientific community and industries in these organisms is driven by the fast-growing world of modern biotechnology. Genomics, transcriptomics, proteomics, metabolomics and their integration collectively termed here as 'marine algal-omics' have broadened the research horizon in view of enhancing human's life by addressing environmental problems and encouraging novelty in the field of pharmaceuticals among so many more. Their use in the human society dates back to 500 B. C. in China and later across the globe; they are still being used for similar purposes and more today. There is a hiking interest in marine algae and their derivatives-from phycoremediation, food supplements, pharmaceuticals to dyes. Marine algae are currently considered as an emerging panacea for the society. They are being studied in a multitude of arenas. The multi-use of marine algae is enticing and promises to be a boon for industrial applications. Yet, most marine algae face challenges that might variably constrain their commercialisation. This chapter gives an overview of marine algae including all the 'omics' technologies involved in studying marine algae and it explores their multitude applications. It also draws the various successful industries budded around them and presents some of the challenges and opportunities along with future directions.

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