Hypersaline Environments of Iran: Prokaryotic Biodiversity and Their Potentials in Microbial Biotechnology

Safarpour, Atefeh; Amoozegar, Mohammad Ali; Ventosa, António

doi:10.1007/978-981-13-0329-6_9

Cited by 8 publications

(3 citation statements)

References 103 publications

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“…Sabkhas, also known as saline soil or evaporites, are good examples of the natural hypersaline environments [ 34 ]. Only halophilic and halotolerant microorganisms thrive in these environments, which have lower microbial diversity than seawater [ 35 ]. The extreme saline conditions in these environments favors the growth of microbes possessing unique adaptive characteristics that could be exploited in a variety of biotechnological applications, particularly hydrolytic enzymes [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate

Fahmy

El-Deeb

2023

Journal of Genetic Engineering and Biotechnology

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Background Hydrolytic enzymes from halophilic microorganisms have a wide range of industrial applications. Herein, we report the isolation of Halobacillus sp. HAL1, a moderately halophilic bacterium that produces a novel high molecular weight extracellular alkaline protease when grown in fish processing wastes as a substrate. Results Results showed that the isolated strain belonged to the genus Halobacillus, and it was designated as Halobacillus sp. HAL1 with the GenBank accession number OK001470. The strain secreted an extracellular alkaline protease, and the highest yield was obtained when it was grown in a medium with fish wastes substrate as the sole nutritional source (10 g/L) and incubated at 25 °C under shaking conditions. The enzyme was partially purified by Sephadex G-100 column chromatography. Zymographic analysis showed two casein degrading bands of about 190 and 250 KDa. The optimum enzyme activity was at a temperature of 50 °C at pH 8. The proteolytic activity was enhanced in the presence of metal ions (Ca2+, Mg2+, and Mn2+), surfactants (Tween 80, SDS, and Triton-X100), H2O2, and EDTA. Conclusion Our study indicates that Haobacillus sp. HAL1 is a moderately halophilic strain and secrets a novel high molecular wight alkaline protease that is suitable for detergent formulation.

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Section: Discussionmentioning

confidence: 99%

Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate

Fahmy

El-Deeb

2023

Journal of Genetic Engineering and Biotechnology

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“…Although bacterial cells have different communication methods with tumor cells other than metabolites experimentally, bacterial metabolites have been considered the most conventional way against cancer cells viability. Today, more attention is focused on extremophiles as a new source of novel biomolecules [104,105]. Among extremophiles, halophilic and halotolerant microorganisms, which inhabit hypersaline environments, are considered as reliable sources of antitumor metabolites with fewer side effects.…”

Section: Anticancer Compoundsmentioning

confidence: 99%

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

2019

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The organisms thriving under extreme conditions better than any other organism living on Earth, fascinate by their hostile growing parameters, physiological features, and their production of valuable bioactive metabolites. This is the case of microorganisms (bacteria, archaea, and fungi) that grow optimally at high salinities and are able to produce biomolecules of pharmaceutical interest for therapeutic applications. As along as the microbiota is being approached by massive sequencing, novel insights are revealing the environmental conditions on which the compounds are produced in the microbial community without more stress than sharing the same substratum with their peers, the salt. In this review are reported the molecules described and produced by halophilic microorganisms with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead compounds and the current aspects on the exploitation and its limitations are discussed.

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“…In this case, our aim was to study the transitions of a primarily freshwater group to saline and marine systems. For this reason, our study took place in athalassohaline systems, which, by definition, are those saline systems that have never been connected to the sea and whose salinity level and composition are different from seawater (Last 2002 , Oren 2007 , Safarpour et al 2018 ). They typically occur in endorheic drainage basins under arid or semiarid climates where evaporation exceeds precipitation (Hammer 1986 , O'leary and Glenn 1994 , Wurtsbaugh et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

When ecological transitions are not so infrequent: independent colonizations of athalassohaline water bodies by Arcellidae (Arcellinida; Amoebozoa), with descriptions of four new species

Useros

González‐Miguéns

Soler‐Zamora

et al. 2023

FEMS Microbiology Ecology

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The salinity and humidity barriers divide biodiversity and strongly influence the distribution of organisms. Crossing them opens the possibility for organisms to colonize new niches and diversify, but requires profound physiological adaptations and is supposed to happen rarely in evolutionary history. We tested the relative importance of each ecological barrier by building the phylogeny, based on mitochondrial cytochrome oxidase gene (COI) sequences, of a group of microorganisms common in freshwater and soils, the Arcellidae (Arcellinida; Amoebozoa). We explored the biodiversity of this family in the sediments of athalassohaline water bodies (i.e. of fluctuating salinity that have non-marine origins). We found three new aquatic species which represent, to our knowledge, the first reports of Arcellinida in these salt impacted ecosystems, plus a fourth terrestrial one in bryophytes. Culturing experiments performed on Arcella euryhalina sp. nov. showed similar growth curves in pure freshwater and under 20g/L salinity, and long term survival at 50g/L, displaying a halotolerant biology. Phylogenetic analyses showed that all three new athalassohaline species represent independent transition events through the salinity barrier by freshwater ancestor, in contrast to the terrestrial species, which are monophyletic and represent a unique ecological transition from freshwater to soil environments.

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Hypersaline Environments of Iran: Prokaryotic Biodiversity and Their Potentials in Microbial Biotechnology

Cited by 8 publications

References 103 publications

Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate

Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

When ecological transitions are not so infrequent: independent colonizations of athalassohaline water bodies by Arcellidae (Arcellinida; Amoebozoa), with descriptions of four new species

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