Hyperspectral darkfield microscopy of single hollow gold nanoparticles for biomedical applications

Fairbairn, Natasha; Christofidou, Agathi; Kanaras, Antonios G.; Newman, Tracey A.; Muskens, Otto L.

doi:10.1039/c2cp43162a

Cited by 54 publications

(39 citation statements)

References 33 publications

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“…The LSPR is extremely sensitive to particle morphology with agglomeration of GNMs resulting in a shift of the LSPR to lower energy [31]. The hsDFM can monitor this shift of scattered photons from GNMs, thereby distinguishing light scattered by the cellular environment from that scattered by nanoparticles [32–36], and this instrument has been used to distinguish macrophage maturity based on silver nanoparticle (AgNPs) uptake [37]. …”

Section: Introductionmentioning

confidence: 99%

Rapid determination of plasmonic nanoparticle agglomeration status in blood

Jenkins

Mudalige

et al. 2015

Biomaterials

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Plasmonic nanomaterials as drug delivery or bio-imaging agents are typically introduced to biological systems through intravenous administration. However, the potential for agglomeration of nanoparticles in biological systems could dramatically affect their pharmacokinetic profile and toxic potential. Development of rapid screening methods to evaluate agglomeration is urgently needed to monitor the physical nature of nanoparticles as they are introduced into blood. Here, we establish novel methods using darkfield microscopy with hyperspectral detection (hsDFM), single particle inductively-coupled plasma mass spectrometry (spICP-MS), and confocal Raman microscopy (cRM) to discriminate gold nanoparticles (AuNPs) and their agglomerates in blood. Rich information about nanoparticle agglomeration in situ is provided by hsDFM monitoring of the plasmon resonance of primary nanoparticles and their agglomerates in whole blood; cRM is an effective complement to hsDFM to detect AuNP agglomerates in minimally manipulated samples. The AuNPs and the particle agglomerates were further distinguished in blood for the first time by quantification of particle mass using spICP-MS with excellent sensitivity and specificity. Furthermore, the agglomeration status of synthesized and commercial NPs incubated in blood was successfully assessed using the developed methods. Together, these complementary methods enable rapid determination of the agglomeration status of plasmonic nanomaterials in biological systems, specifically blood.

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Section: Introductionmentioning

confidence: 99%

Rapid determination of plasmonic nanoparticle agglomeration status in blood

Jenkins

Mudalige

et al. 2015

Biomaterials

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“…Illumination intensities com patible with livecell imaging and the possibility of analysing hydrated samples enable investigation of nanoparticle transformations and their interactions with living cells. Hyperspectral imaging has been successfully applied to the detection of nano particles in complex samples 37 , and to the characterization of intracellular nano particles in both the biomedical 38 and environmental fields 39 . Xray fluorescence mapping and tomographic approaches are increasingly used to study the 2D and 3D elemental distribution in biological systems (reviewed in refs 40,41).…”

Section: Perspectivementioning

confidence: 99%

Bridging the divide between human and environmental nanotoxicology

2015

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The need to assess the human and environmental risks of nanoscale materials has prompted the development of new metrological tools for their detection, quantification and characterization. Some of these methods have tremendous potential for use in various scenarios of nanotoxicology. However, in some cases, the limited dialogue between environmental scientists and human toxicologists has hampered the full exploitation of these resources. Here we review recent progress in the development of methods for nanomaterial analysis and discuss the use of these methods in environmental and human toxicology. We highlight the opportunities for collaboration between these two research areas.

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“…The combination of low illumination power and fast integration times made the system highly suitable for the characterization of the interaction between NPs and cells. [15] Two photon photoluminescence (TPPL) has also successfully been used on skin cross sections to provide a direct visualization of NP migration in skin substructures. While this technique does not provide detailed information on the exact number of particles, TPPL was shown to give a global overview of the distribution of NPs throughout the skin layers.…”

Section: Introductionmentioning

confidence: 99%