2001
DOI: 10.13031/2013.6099
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Hyperspectral Reflectance and Fluorescence Imaging System for Food Quality and Safety

Abstract: The spectral range is from 430 to 930 nm with spectral resolution of approximately 10 nm (full width at half maximum) and spatial resolution better than 1 mm. Our system is capable of reflectance and fluorescence measurements with the use of dual illumination sources where fluorescence emissions are measured with ultraviolet (UV-A) excitation. We present the calibrations and image-correction procedures for the system artifacts and heterogeneous responses caused by the optics, sensor, and lighting conditions th… Show more

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Cited by 122 publications
(28 citation statements)
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“…Before the fluorescence hyperspectral image acquisition, highpass-filter was placed in front of the camera lens to prevent transmittance of radiations smaller than 455 nm, so as to remove spectral contamination caused by pseudo-fluorescence (Kim, Chen, & Mehl, 2001). Therefore, the effective fluorescence spectrum collected by the spectrometer ranged from 455 to 1000 nm.…”
Section: Image Acquisition and Data Extractionmentioning
confidence: 99%
“…Before the fluorescence hyperspectral image acquisition, highpass-filter was placed in front of the camera lens to prevent transmittance of radiations smaller than 455 nm, so as to remove spectral contamination caused by pseudo-fluorescence (Kim, Chen, & Mehl, 2001). Therefore, the effective fluorescence spectrum collected by the spectrometer ranged from 455 to 1000 nm.…”
Section: Image Acquisition and Data Extractionmentioning
confidence: 99%
“…The entire process of image acquisition was operated in the dark cover to avoid the influence of external light. First, a high pass filter was placed in front of the camera lens to prevent transmittance of radiations smaller than 475 nm, so as to remove spectral F I G U R E 1 The structure of the fluorescence hyperspectral imaging system contamination caused by pseudo-fluorescence (Kim, Chen, & Mehl, 2001). Therefore, the hyperspectral image was captured only in the wavelength range of 475 to 1,000 nm.…”
Section: Fluorescence Hyperspectral Image Acquisitionmentioning
confidence: 99%
“…Light was transmitted via two optical fibers to the sample surfaces to provide near-uniform illumination. The detailed information of system was described by Kim et al [28].…”
Section: Hyperspectral Image Acquisitionmentioning
confidence: 99%
“…In order to extract the actual spectral response of the samples, the influence of both the white and dark current image was removed and thus the calibrated image, I R , was achieved by the following equation [28].…”
Section: Data Extraction and Pretreatment Of Spectramentioning
confidence: 99%