Hyphal Wall Chemistry of Leptomitus lacteus

Aronson, Jerome M.; Lin, C. C.

doi:10.2307/3759034

Cited by 22 publications

(11 citation statements)

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“…6)-glucans (Bartnicki-Garcia, 1968). As opposed to fungal species, only a few Oomycetes have been reported to contain chitin or soluble chito-oligosaccharides in their cell walls (Aronson and Lin, 1978;Badreddine et al, 2008;Bartnicki-Garcia, 1968;Bulone et al, 1992;Lin and Aronson, 1970). In all cases, the chitin found in Oomycetes never exceeds a few percent of the total cell wall carbohydrates.…”

Section: Introductionmentioning

confidence: 93%

Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

Fugelstad

Bouzenzana

Djerbi

et al. 2009

Fungal Genetics and Biology

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Section: Introductionmentioning

confidence: 93%

Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

Fugelstad

Bouzenzana

Djerbi

et al. 2009

Fungal Genetics and Biology

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“…atropurpurea, large quantities of thalli (äs noted above) were treated with methanolic-KOH (5% KOH in 80% methanol) to achieve rapid removal of cytoplasmic constituents. Details of this chemical wall Isolation procedure have been published (Aronson and Lin 1978).…”

Section: Cultivationmentioning

confidence: 99%

Cell Wall Composition of the Generic Phase of Bangia atropurpurea (Rhodophyta)

Gretz

Sommerfeld²,

Aronson

1982

Botanica Marina

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Mechanically isolated cell walls were prepared from the generic phase of Bangia atropurpurea and chemically characterized. Electron microscopic examination of cell wall preparations revealed them to be morphologically similar to walls of whole thalli and free from protoplasmic contamination. The chemical composition of over 92% of the total wall weight was determined and polysaccharides were the major wall components (72% of wall weight). Mannan (at least part of which is 0-1,4-linked) and a water soluble galactan fraction consisting of both D-and L-galactose residues in a ratio of l: 1.1 represents a large proportion of the carbohydrate content of isolated cell walls. In addition, 3,6-anhydrogalactose is probably associated with the water-soluble galactoses. The presence of cellulose could not be demonstrated. Protein was a significant wall component (10.6% of total wall weight) and consisted of at least 16 aminoacids, including hydroxyproline (0.3% of wall weight). Walls also contained 8.4% lipid and l .4% ash.

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“…The cell wall is extended and thickened during germination of the cyst and subsequent production of the appressorium and is therefore important for the establishment of infection. The cell walls of oomycetes consist essentially of (1/3)-b-D-glucans, (1/6)-b-D-glucans, and cellulose (Bartnicki-Garcia, 1968), whereas chitin, which is a major cell wall component of true fungi, occurs in very small amounts in the walls of several oomycetes (Lin and Aronson, 1970;Aronson and Lin, 1978;Campos-Takaki et al, 1982;Bulone et al, 1992). Cellulose has a microfibrillar structure in the walls of oomycetes (Bulone et al, 1992;Helbert et al, 1997) and is thought to contribute in scaffolding in a similar manner as chitin does in the walls of true fungi (Bartnicki-Garcia and Wang, 1983).…”

Section: Introductionmentioning

confidence: 99%

Cellulose Synthesis inPhytophthora infestansIs Required for Normal Appressorium Formation and Successful Infection of Potato

et al. 2008

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Cellulose, the important structural compound of cell walls, provides strength and rigidity to cells of numerous organisms. Here, we functionally characterize four cellulose synthase genes (CesA) in the oomycete plant pathogen Phytophthora infestans, the causal agent of potato (Solanum tuberosum) late blight. Three members of this new protein family contain Pleckstrin homology domains and form a distinct phylogenetic group most closely related to the cellulose synthases of cyanobacteria. Expression of all four genes is coordinately upregulated during pre-and early infection stages of potato. Inhibition of cellulose synthesis by 2,6-dichlorobenzonitrile leads to a dramatic reduction in the number of normal germ tubes with appressoria, severe disruption of the cell wall in the preinfection structures, and a complete loss of pathogenicity. Silencing of the entire gene family in P. infestans with RNA interference leads to a similar disruption of the cell wall surrounding appressoria and an inability to form typical functional appressoria. In addition, the cellulose content of the cell walls of the silenced lines is >50% lower than in the walls of the nonsilenced lines. Our data demonstrate that the isolated genes are involved in cellulose biosynthesis and that cellulose synthesis is essential for infection by P. infestans.

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Hyphal Wall Chemistry of Leptomitus lacteus

Cited by 22 publications

References 0 publications

Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

Cell Wall Composition of the Generic Phase of Bangia atropurpurea (Rhodophyta)

Cellulose Synthesis inPhytophthora infestansIs Required for Normal Appressorium Formation and Successful Infection of Potato

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