Hyphenated chromatographic techniques for the rapid screening and identification of antioxidants in methanolic extracts of pharmaceutically used plants

Exarchou, Vassiliki; Fiamegos, Yiannis C.; Beek, T.A. van; Nanos, Christos G.; Vervoort, Jacques

doi:10.1016/j.chroma.2005.11.077

Cited by 102 publications

(56 citation statements)

References 41 publications

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“…Hyphenation with MS and/or NMR is also possible and allows on-line structure elucidation. A nice example is the investigation of a Lonicera extract (Exarchou et al 2006). For this investigation a twoway valve in between the diode-array detector and the DPPH syringe pump was mounted.…”

Section: High-resolution Screeningmentioning

confidence: 99%

“…After recording of the NMR spectra, the content of the LC/NMR probe was collected and infusion ESI-MS and MS/MS was performed yielding molecular weights and fragmentation patterns. With this information the two peaks could be identified as the known chlorogenic acid and 3 0 -caffeoyl-chlorogenic acid respectively (Exarchou et al 2006). In the whole process no preparative separation step occurred.…”

Section: High-resolution Screeningmentioning

confidence: 99%

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Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

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Section: High-resolution Screeningmentioning

confidence: 99%

Section: High-resolution Screeningmentioning

confidence: 99%

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

et al. 2009

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“…Other research suggests that flavonoids 38 , triterpenes, vitamin, and polysaccharides 36,39 were the main active ingredient of C.…”

Section: Preliminary Phytochemical Analysis Of the Crude Extractsmentioning

confidence: 96%

Essential Oils and Crude Extracts from Chrysanthemum trifurcatum Leaves, Stems and Roots: Chemical Composition and Antibacterial Activity

Sassi¹,

Harzallah‐Skhiri

Chraief

et al. 2014

J. Oleo Sci.

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“…As shown in the literature research above, this is a very well documented and widely used approach. To test the feasibility of this approach, it is subjected to a detailed review:  Analytical-scale separation and determination of molecular structure is a very well-researched area in the scientific field of substance discovery in plant extracts [40][41][42][43][44][45][46]. Nevertheless, these techniques are optimized with the focus being to identify one or at least a few substances.…”

Section: Solid-liquid-extractionmentioning

confidence: 99%

Characterization of Feed Properties for Conceptual Process Design Involving Complex Mixtures, Such as Natural Extracts

Josch¹,

Both²,

Strube³

2012

FNS

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The number of products used as agro-chemicals, food additives, flavors, aromas, pharmaceuticals and nutraceuticals which are made by fermentation or extraction from plants has increased significantly. Despite this growth, initial predictions for a potential product purification process for these complex mixtures remains entirely experimentally based. The present work represents an initial study to demonstrate the benefits of a systematic approach. For process development of chemically well-studied systems model based process design methods are already available. Therefore the proposed approach focuses on a method for the efficient characterization of the physical properties of the key components. Once this is adequately defined, unit operations and their potential to separate the feed components can be modeled. The current state of research is discussed. Based on this evaluation the most efficient method for conceptual process development has been identified and further developed. The resulting methodology consists of model-based cost accounting accompanied by experimental model-parameter determination. The latter is carried out at in miniaturized laboratory-scale measurement cells for each unit operation using the complete original feed. The model-based modelparameter determination from these experiments is accompanied by a comprehensive error analysis. The experimental plan currently includes the determination of thermodynamic equilibrium conditions in the mixture directly from the raw material mixture. Transport kinetics and fluid dynamic parameters are first estimated from known correlations or preexisting knowledge. Later on these parameters are determined exactly in mini-plant experiments. Furthermore, biological and botanical-based guidelines are developed to identify thermodynamically favored basic operations. Finally, the developed approaches are successfully validated using two plant extracts. Firstly, it could be proven that the botanical pre-selection can reduce the experimental plan significantly. Secondly, it was shown that the experimental equilibrium data of the kinetics and fluid dynamics can have a significant impact on the separation costs. Therefore, detailed rigorous modeling approaches have to be chosen instead of short-cut methods in order to make any valid process development conclusions or to further optimize the system.

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Hyphenated chromatographic techniques for the rapid screening and identification of antioxidants in methanolic extracts of pharmaceutically used plants

Cited by 102 publications

References 41 publications

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Recent developments in the rapid analysis of plants and tracking their bioactive constituents

Essential Oils and Crude Extracts from Chrysanthemum trifurcatum Leaves, Stems and Roots: Chemical Composition and Antibacterial Activity

Characterization of Feed Properties for Conceptual Process Design Involving Complex Mixtures, Such as Natural Extracts

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