Hypocotyl Reactions and Glyceollin in Soybeans Inoculated with Zoospores of<i>Phytophthora megasperma</i>var.<i>sojae</i>

Ward, E. W. B.

doi:10.1094/phyto-69-951

Cited by 84 publications

(65 citation statements)

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“…Some increase in lesion size occurred in hypocotyls treated with 5 x 10-5 M ABA, but at this concentration the lesions were similar to controls in that they were dark brown with superficial necrosis. Lesions were much more extensive following treatment with higher concentrations of ABA, and those that developed at 2 x 10-M ABA were pale in color and resembled lesions that develop in hypocotyls of susceptible seedlings (22). Gly- (Table I).…”

Section: Resultsmentioning

confidence: 99%

“…Kuan and Erwin race 1 was that used previously (20). It was maintained on V8 juice agar plates at 25°C in the dark, and zoospore suspensions (105 mL-') were produced from the cultures as described previously (22).…”

Section: Materials and Methods Pathogen And Hostmentioning

confidence: 99%

“…Seedlings of soybeans, cv Harosoy 63 (resistant to race 1), were grown in the dark for 6 d as described previously (22).…”

Section: Materials and Methods Pathogen And Hostmentioning

confidence: 99%

“…Treated hypocotyls were arranged horizontally in glass trays with the cut ends covered by moist cellucotton (22). They were inoculated by placing 10 ,uL drops of zoospore suspension or sterile distilled water on to the hypocotyl surface about 2 cm below the cotyledons.…”

Section: Inoculation and Incubationmentioning

confidence: 99%

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Abscisic Acid Suppression of Phenylalanine Ammonia-Lyase Activity and mRNA, and Resistance of Soybeans to Phytophthora megasperma f.sp. glycinea

Ward¹,

Cahill²,

Bhattacharyya³

1989

Plant Physiol.

125

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Etiolated hypocotyls of the resistant soybean (Glycine max [L.] Merr.) cultivar Harosoy 63 became susceptible to Phytophthora megasperma (Drechs.) f.sp. glycinea (Hildeb.) Kuan and Erwin race 1 after treatment with abscisic acid. Susceptibility was expressed by increases in lesion size and a major decrease in accumulation of the isoflavonoid phytoalexin, glyceollin. In untreated hypocotyls, activity of phenylalanine ammonia-lyase and accumulation of mRNA for this enzyme increased rapidly after infection, but these increases were suppressed in abscisic acidtreated hypocotyls. The results suggest the possibility that biosynthesis of glyceollin in the resistance response of soybeans may be controlled at the transcriptional level by changes in abscisic acid concentrations caused by infection.

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Methods Pathogen And Hostmentioning

confidence: 99%

“…Seedlings of soybeans, cv Harosoy 63 (resistant to race 1), were grown in the dark for 6 d as described previously (22).…”

Section: Materials and Methods Pathogen And Hostmentioning

confidence: 99%

Section: Inoculation and Incubationmentioning

confidence: 99%

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Abscisic Acid Suppression of Phenylalanine Ammonia-Lyase Activity and mRNA, and Resistance of Soybeans to Phytophthora megasperma f.sp. glycinea

Ward¹,

Cahill²,

Bhattacharyya³

1989

Plant Physiol.

125

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“…24 Briefly, the pathogen was grown on V8 solid medium for 5 days at 22 C and soaked overnight with sterile double distilled water. Following day, P. sojae plates were washed repeatedly to deplete nutrients of the medium so that sporangia were formed for production of zoospores in the following morning.…”

Section: B Microtubing For Microfluidic Devicementioning

confidence: 99%

Microfluidic droplet encapsulation of highly motile single zoospores for phenotypic screening of an antioomycete chemical

et al. 2011

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Highly motile Phytophthora sojae (P. sojae) zoospores of an oomycete plant pathogen and antioomycete candidate chemicals were encapsulated into microdroplets. Random fast self-motion of P. sojae zoospores was overcome by choosing an appropriate flow rate for a zoospore suspension. To influence stochastic loading of zoospores into a microfluidic channel, a zoospore suspension was directly preloaded into a microtubing with a largely reduced inner diameter. A relatively high single zoospore encapsulation rate of 60.5% was achieved on a most trivial T-junction droplet generator platform, without involving any specially designed channel geometry. We speculated that spatial reduction in the diameter direction of microtubing added a degree of zoospore ordering in the longitudinal direction of microtubing and thus influenced positively to change the inherent limitation of stochastic encapsulation of zoospores. Comparative phenotypic study of a plant oomycete pathogen at a single zoospore level had not been achieved earlier.Phenotypic changes of zoospores responding to various chemical concentration conditions were measured in multiple droplets in parallel, providing a reliable data set and thus an improved statistic at a low chemical consumption. Since each droplet compartment contained a single zoospore, we were able to track the germinating history of individual zoospores without being interfered by other germinating zoospores, achieving a high spatial resolution. By adapting some existing droplet immobilization and concentration gradient generation techniques, the droplet approach could potentially lead to a medium-to-high throughput, reliable screening assay for chemicals against many other highly motile zoospores of pathogens.

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Breeding Soybeans Resistant to Diseases

Wilcox

1983

Plant Breeding Reviews

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Hypocotyl Reactions and Glyceollin in Soybeans Inoculated with Zoospores ofPhytophthora megaspermavar.sojae

Cited by 84 publications

References 0 publications

Abscisic Acid Suppression of Phenylalanine Ammonia-Lyase Activity and mRNA, and Resistance of Soybeans to Phytophthora megasperma f.sp. glycinea

Abscisic Acid Suppression of Phenylalanine Ammonia-Lyase Activity and mRNA, and Resistance of Soybeans to Phytophthora megasperma f.sp. glycinea

Microfluidic droplet encapsulation of highly motile single zoospores for phenotypic screening of an antioomycete chemical

Breeding Soybeans Resistant to Diseases

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