Hypoglycemic and Hypolipidemic Effect of Methanol Extract of Hibiscus Sabdariffa Seed in Alloxan Induced Diabetic Albino Rats

Abstract

<jats:p>This study investigated the hypoglycemic and hypolipidemic effect of the oral administration of methanol extract of Hibiscus sabdariffa seed in alloxan induced diabetic albino rats. Two different doses (200 and 400 mg/kg) of the methanol extract of Hibiscus sabdariffa seed were administered daily to two groups of diabetic rats for a period of two weeks. Metformin (250 mg/kg) was given as the reference standard drug. Phytochemical, acute toxicity, fasting blood glucose, body weight, cholesterol, triglycerides and lipoproteins levels were determined using standard methods. Acute toxicity of methanol extract of Hibiscus sabdariffa seed was estimated to be greater than 5000 mg/kg body weight. There was an initial decrease in the body weight of the experimental animals after induction of diabetes which increased significantly (p&lt;0.05) by the second week of treatment. The fasting blood glucose levels of the treated diabetic rats were significantly decreased at the second week of treatment with the group that received 400 mg/kg of extract recording the lowest fasting blood glucose level. The cholesterol, triglycerides and low density lipoproteins levels of the diabetic treated rats were significantly reduced while the high density lipoproteins level significantly increased. It can therefore be concluded that Hibiscus sabdariffa seed has the ability to lower blood sugar and maintain a stable lipid level.&#x0D; Keywords: Hypoglycemic, hypolipidemic, Alloxan, Phytochemical, Hibiscus sabdariffa</jats:p>

Beneficial Effects of Hibiscus sabdariffa in Diabetic Wistar Rats

“…The finding in this study is in agreement with the reported findings in a similar study that indicated that Hibiscus sabdariffa calyx extract caused significant (p < 0.05) reduction in blood glucose level [30].…”

Antidiabetic potential of Hibiscus sabdariffa extract in alloxan-induced diabetic rats

“…Methanolic extract of H. sabdariffa calyces showed positive effect in the regeneration of pancreatic beta-cells in streptozotocin-induced diabetes type-1 in rats [17]. In addition, hypoglycemic and hypolipidemic properties were found in alloxan-induced diabetic rats after oral administration of leaves [18] or seed [19] methanolic extract. However, efficacy studies of H. sabdariffa calyces extract against diabetes are relatively few even as calyces of H. sabdariffa have been shown to better promote the antioxidant capacity compared to the leaves [20].…”

Hibiscus sabdariffa in Diabetes Prevention and Treatment—Does It Work? An Evidence-Based Review

“…The aim of the work is a review of the literature describing the use of roselle in the treatment of diabetes mellitus and its accompanying diseases. Roselle has been the subject of many original and reviewed papers, including conditions that may be associated with diabetes and metabolic syndrome [ 35 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ]. However, the existing works are older or incompletely related to diabetes, and research progress on this topic is very large [ 69 , 70 , 71 , 72 ].…”

“…Apart from the calyces and flowers of roselle that are widely described in the literature, one can also find publications describing the beneficial effects of extracts from the leaves, stems, and seeds of this plant [ 54 , 60 , 177 ]. Studies show that methanolic roselle leaf extracts, when administered to rats with alloxan-induced diabetes, had an antidiabetic effect and lowered cholesterol and triglycerides levels compared to the untreated controls [ 60 , 177 ].…”

“…Similar properties were demonstrated for methanolic extracts from roselle stems [ 60 ]. Methanolic extracts from seeds also showed a hypoglycemic effect and reduced cholesterol, triglycerides, and LDL levels [ 54 ]. Therefore, it can be concluded that these plant parts are also effective in reducing the effects of hyperglycemia and dyslipidemia ( Figure 2 ) [ 54 , 60 , 177 ].…”