Hypoglycemic effect of crude exopolysaccharides produced byCerrena unicolor,Coprinus comatus, andLenzites betulinaisolates in streptozotocin- induced diabetic rats

Yamaç, Mustafa; Zeytinoğlu, Melih; Kanbak, Güngör; Bayramoğlu, Gökhan; Şentürk, Hakan

doi:10.1080/13880200802436950

Cited by 27 publications

(10 citation statements)

References 26 publications

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“…Recent studies described in vivo antidiabetic activity of different extracts and fractions of C. comatus (Yamaç et al, 2009;Ding et al, 2010;Zhou et al, 2015). According to our knowledge, no previous records were published on α-amylase and α-glucosidase enzyme inhibition with C. comatus methanolic extract.…”

Section: Resultsmentioning

confidence: 95%

An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes

Stojković

Smiljković

Ćirić

et al. 2019

South African Journal of Botany

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As a continuation of our search for biologically active mushroom species the present study investigates in vitro antidiabetic properties of six edible and medicinal mushroom species: Agaricus blazei Murrill, Coprinus comatus (O.F.Müll.) Pers., Cordyceps militaris (L.) Fr., Inonotus obliquus (Ach. ex Pers.) Pilát, Morchella conica Pers. and Phellinus linteus Berk. & M.A. Curtis. In vitro assays on α-amylase and α-glucosidase enzyme inhibition were performed with methanolic extracts of the selected mushrooms. Furthermore, we calculated the necessary daily intake of mushroom extracts and dry mushroom powders based on the equivalent doses of therapeutic drug acarbose given to diabetic patients per day. Our comparative study on enzyme inhibition showed that the most promising potential is ascribed to I. obliquus extract, while no inhibition of α-amylase was recorded with M. conica and C. militaris methanolic extract at the tested concentration. This comparative study is the first highlighting in vitro antidiabetic potential by inhibition of α-amylase and α-glucosidase with methanolic extracts; which makes the investigated species more promising for the diabetes type-2 treatment by an additional and different mechanism of action.

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Section: Resultsmentioning

confidence: 95%

An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes

Stojković

Smiljković

Ćirić

et al. 2019

South African Journal of Botany

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“…Lenzites betulina is a macro fungus belonging to the order Aphyllophorales and family Polyporaceae, which is widely distributed in China. Modern pharmacological studies have shown that L. betulina had anticancer properties, antibiotic activity, antioxidant activity, and played an important role in the prevention of STZ‐induced diabetes . More small molecule compounds have been isolated from L. betulina , which have shown moderate antibiotic activity and could be a good anticancer and antibiotic agent …”

Section: Introductionmentioning

confidence: 99%

Optimization of the Cellulase−Ultrasonic Synergistic Extraction Conditions of Polysaccharides from Lenzites betulina

Guo

Tan

Hui

et al. 2019

Chemistry & Biodiversity

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Lenzites betulina has been recognized as a rich source of chemical components, including polysaccharides, sterides and sugar alcohols. In this study, cellulase−ultrasonic synergistic extraction method was applied to extract polysaccharides from L. betulina, and the response surface methodology was used to optimize the extraction conditions. The eight basic factors affecting extraction yield were evaluated by Plackett−Burman design (PBD). Then, the four important factors significantly affecting the yield of polysaccharides from L. betulina, including enzymolysis temperature, enzymolysis time, ultrasound time and ultrasound temperature, were optimized by Box−Behnken design (BBD). Maximum extraction yield of L. betulina polysaccharides was 13.64±0.09 % at a cellulase dosage of 0.8 %, enzymolysis temperature of 60 °C, enzymolysis time of 180 min, pH of 4.5, liquid‐solid ratio of 45 ml/g, ultrasound power of 300 W, ultrasound time of 20 min and ultrasound temperature of 45 °C. Subsequently, the characteristic structure of crude polysaccharides was determined by FT‐IR. Results indicated that cellulase−ultrasonic synergistic treatment is suitable for L. betulina polysaccharides extraction, and it has good prospect for development and utilization.

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“…This fungus commonly called “mossy maze polypore” may be found on dead northern hardwood tree species as maple, birch, or alder, where it causes white rot [ 8 ]. Besides extracellular enzymes, C. unicolor may be a source of polysaccharides [ 9 ] or low molecular fractions of secondary metabolites [ 10 ], which possess interesting biomedical and bioelectrochemical properties. However, up to date, cellobiose dehydrogenase, which was proven a crucial enzyme in decomposition of both cellulose and lignin, has not been described in cultures of the genus Cerrena .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain

Sulej

Janusz

Osińska-Jaroszuk

et al. 2015

Appl Biochem Biotechnol

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Cellobiose dehydrogenase (CDH), a secreted flavocytochrome produced by a number of wood-degrading fungi, was detected in the culture supernatant of a biotechnologically important strain of Cerrena unicolor grown in a modified cellulose-based liquid medium. The enzyme was purified as two active fractions: CuCDH-FAD (flavin domain) (1.51-fold) with recovery of 8.35 % and CuCDH (flavo-heme enzyme) (21.21-fold) with recovery of 73.41 %. As CDH from other wood-rotting fungi, the intact form of cellobiose dehydrogenase of C. unicolor is a monomeric protein containing one flavin and one heme b with molecular mass 97 kDa and pI = 4.55. The enzyme is glycosylated (8.2 %) mainly with mannose and glucosamine residues. Moreover, the cellobiose dehydrogenase gene cdh1 and its corresponding cDNA from the fungus C. unicolor were isolated, cloned, and characterized. The 2316-bp full-length cDNA of cdh1 encoded a mature CDH protein containing 771 amino acids preceded by a signal peptide consisting of 18 amino acids. Moreover, both active fractions were characterized in terms of kinetics, temperature and pH optima, and antioxidant properties.

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Hypoglycemic effect of crude exopolysaccharides produced byCerrena unicolor,Coprinus comatus, andLenzites betulinaisolates in streptozotocin- induced diabetic rats

Cited by 27 publications

References 26 publications

An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes

An insight into antidiabetic properties of six medicinal and edible mushrooms: Inhibition of α-amylase and α-glucosidase linked to type-2 diabetes

Optimization of the Cellulase−Ultrasonic Synergistic Extraction Conditions of Polysaccharides from Lenzites betulina

Characterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain

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