Hypomiltin, a novel azaphilone from Hypoxylon hypomiltum, and chemotypes in Hypoxylon sect. Hypoxylon as inferred from analytical HPLC profiling

Recognition of hypoxyloid and xylarioid Entonaema species and allied Xylaria species from a comparison of holomorphic morphology, HPLC profiles, and ribosomal DNA sequences

“…2, which are referred to in the text by bold numbers as defined there) and a database of HPLC profiles were available from previous work Stadler and Fournier 2006;and references therein). HPLC and other spectral techniques were carried out as described in Hellwig et al (2005). Non-invasive HPLC studies on irreplaceable type specimens were carried out, using the extraction protocol described in Stadler et al (2004b), and quantification of secondary metabolites was performed as described by Stadler et al (2007b).…”

“…and Hypoxylon. Meanwhile, chemotaxonomic studies (Stadler et al , 2004aHellwig et al 2005;Quang et al 2005) using high performance liquid chromatography (HPLC) with diode array detection (DAD) and electrospray ionisationbased mass spectrometric detection (ESI-MS) allowed for a survey of characteristic chemical traits in the aforementioned genera, even providing conclusive results based on ancient specimens. This study revealed that several specific marker molecules of Xylariaceae remained stable for centuries in their herbarium specimens, and that cultures of two Entonaema spp.…”

Lepraric acid derivatives as chemotaxonomic markers in Hypoxylon aeruginosum, Chlorostroma subcubisporum and C. cyaninum, sp. nov.

“…For the chemotaxonomic studies, minute amounts of stromatal material containing pigment granules were prepared under a dissecting microscope and extracted with methanol. Metabolite profiling was carried out using a dual analytical hplc system, comprising a diode array detector (DAD) and mass spectrometric detectors, the latter providing mass spectra in the positive and negative electrospray ionisation (ESI) mode (see Hellwig et al 2005 for the hplc-DAD based methodology and Bitzer et al 2007 for the hplc-MS dereplication methodology). The resulting chromatograms, MS and UV/visible spectra were used to identify the secondary metabolites in the crude extracts as previously described (Quang et al 2005, 2007.…”

A new species of Daldinia (Xylariaceae) from the Argentine subtropical montane forest

“…appears to be closely related to the new species but differs in having green olivaceous KOH-extractable pigments and by the absence of concentric zones in its subperithecial tissue. In addition, its stromata contain daldinone A as major secondary metabolite (Hellwig et al 2005). The discovery of the new species provides additional evidence that some members of the "H. placentiforme complex" sensu Ju and Rogers (1996) Bitzer et al (2008) reported a somewhat aberrant specimen matching the features of H. placentiforme sensu Ju & Rogers (1996) from South Africa (MUCL 47603) with larger amounts of BNT (and purple stromatal pigments as verified in the current study).…”