Hypophysectomy and a replacement therapy in the rat

Smith, Philip E.

doi:10.1002/aja.1000450203

Cited by 560 publications

(112 citation statements)

References 37 publications

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“…Surgical hypophysectomy in rodents requires skill and experience. The parapharyngeal approach to remove the pituitary gland in rodents has been used traditionally (218,219). This approach has been largely supplanted by the transauricular approach (220,221).…”

Section: And Recommendation 20asupporting

confidence: 42%

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

Bianco

Anderson

Forrest

et al. 2014

Thyroid

175

106

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Section: And Recommendation 20asupporting

confidence: 42%

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

Bianco

Anderson

Forrest

et al. 2014

Thyroid

175

106

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“…Hypophysectomy of the rat causes a depression of spermatogenesis (Smith 1927(Smith , 1930 and produces a testis, at 32 days post-hypophysectomy, that contains mainly early primary spermatocytes, spermatogonia and Sertoli cells (Elkington et al 1973;Elkington and Blackshaw 1974). After hypophysectomy there was a fall in total LDH-X activity at 8 days, which coincided with the initial depression in primary spermatocyte and spermatid numbers.…”

Section: Discussionmentioning

confidence: 45%

Studies on Testicular Function III. Effects of FSH, LH, Testosterone and Dihydrotestosterone on Restoration and Maintenance of Testicular-Specific Lactate Dehydrogenase Activity in the Hypophysectomized Rat

Elkington¹,

Blackshaw²

1974

Aust. Jnl. Of Bio. Sci.

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Hypophysectomy of the adult male rat depressed the activity of testicular-specific lactate dehydrogenase (LDH-X). Groups of rats were treated with FSH (F), LH (L) or testosterone (T) alone or in combination after 32 days of post-hypophysectomy regression (restoration experiment) for a further 32 days or with F, T or dihydrotestosterone (D) alone or in combination from 2 days after hypophysectomy (maintenance experiment) for 30 days.LDH-X activity per milligram of protein was restored to normal by the hormonal combinations FT and LT while F, L, or T given alone or the FL and FLT combinations were ineffective. However, the total LDH-X activity per testis was only partially restored by the hormonal combinations FT, LT and FLT.In the maintenance experiment LDH-X activity per milligram of protein was maintained by all hormonal treatments except D, while the total LDH-X activity per testis was completely maintained by the FT hormonal combination. All other hormonal treatments except F alone maintained the activity of this isoenzyme above the level in the hypophysectomized rat.The restoration and maintenance of LDH-X activity in the testis of the hypophysectomized rat was successful with those combinations of hormones that were also able to restore or maintain the numbers of late primary spermatocytes and spermatids. Thus, the level of LDH-X activity in the testis is dependent on the presence of late primary spermatocytes and spermatids and hence this enzyme is a good marker for the production of these cells in the rat testis.

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“…Also thyroidectomized foetuses have a very low metabolic rate (Hopkins and Thorburn 1972) so hypophysectomy, which results in depressed thyroid function, could facilitate the accumulation of lipid. There is, however, some doubt about whether the obesity that follows hypophysectomy in rats and dogs is due to the absence of the pituitary or due to hypothalamic damage (Smith 1930).…”

Section: Discussionmentioning

confidence: 47%

Quantitative Development of Adipose Tissue in Foetal Sheep

Alexander¹

1978

Aust. Jnl. Of Bio. Sci.

100

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The development of lipid stores in foetal sheep was examined using quantitative dissection and chloroform extraction.The accumulation of extractable lipid commenced prior to the 50th day of foetal age; growth of dissectible perirenal-abdominal adipose tissue and other non-subcutaneous depots (all brown fat) commenced at about day 70, while growth of the subcutaneous adipose tissue (white fat) commenced 2-3 weeks later.The subcutaneous fat regressed markedly from about day 115 and had virtually disappeared by full term. There were also consistent trends (not all statistically significant) in various indices of lipid status indicating that the rate of lipid accumulation was declining near term; these indices include percentage of lipid in adipose tissue, in the body as a whole and in skeletal muscle, and the rate of growth of the perirenal-abdominal adipose depot.The involution of subcutaneous adipose tissue appeared to be hastened by maternal nutritional restriction, but was not prevented by feeding the ewes ad libitum on a high-energy diet. There were also consistently lower values of the indices of lipid status in foetuses from nutritionally restricted ewes than in foetuses from ewes fed ad libitum.The early regression of the white subcutaneous adipose tissue suggests that the normal foetal lamb may be in a state of undernutrition during the last 5 or so weeks of pregnancy.Hypophysectomized foetuses, full term or post-term, were comparatively obese, with a thick layer of subcutaneous fat, a high proportion of other adipose tissue depots in the body, and a high content of chloroform-extractable lipid.

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Hypophysectomy and a replacement therapy in the rat

Cited by 560 publications

References 37 publications

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

American Thyroid Association Guide to Investigating Thyroid Hormone Economy and Action in Rodent and Cell Models

Studies on Testicular Function III. Effects of FSH, LH, Testosterone and Dihydrotestosterone on Restoration and Maintenance of Testicular-Specific Lactate Dehydrogenase Activity in the Hypophysectomized Rat

Quantitative Development of Adipose Tissue in Foetal Sheep

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