Hypothalamic control of prolactin release in the rainbow trout,Salmo gairdneri: in vitro studies

Gonnet, F.; Barret, Alain; Grouselle, Dominique; Prunet, Patrick

doi:10.1007/bf00004721

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…This is in agree¬ ment with a study on prolactin and GH release from the pituitary of rainbow trout in culture (Yada et al 1991). These authors concluded a dominant stimula¬ tory control of rainbow trout prolactin cells by the hypothalamus, as suggested by Gönnet et al (1989), and a dominant inhibitory control for GH cells. Such particular dominant controls of prolactin and GH secretion in rainbow trout, the converse of those described in mammals, are reflected in the present time-course study of the effects of SRIF on prolactin and GH release from trout pituitary cells in culture.…”

Section: Discussionsupporting

confidence: 87%

Effect of somatostatin on prolactin in rainbow trout (Oncorhynchus mykiss) pituitary cells in primary culture

Goff¹,

Weil²,

Valotaire³

et al. 1992

Journal of Molecular Endocrinology

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To study the control of prolactin secretion in fish, an in-vitro technique using a monolayer cell culture system of rainbow trout pituitary glands was developed. Such secretion was characterized by measurement of both prolactin release and prolactin mRNA content using a trout prolactin cDNA as a probe. This cell culture technique, already used to study the regulation of gonadotrophin secretion in rainbow trout, was further validated by measuring total DNA and protein content. Both parameters appeared to be stable after 2 days of culture. Studying the effect of somatostatin (SRIF) on prolactin cells indicated that a maximal inhibitory effect (62%) was observed after 24 h of treatment. Significant inhibition of prolactin release was obtained for SRIF doses ranging from 50 nM to 1 microM. However, in the same experiment, SRIF was much more potent as an inhibitor of growth hormone release. Short-term (< 12 h) incubation with SRIF did not induce a significant change in prolactin release, whereas growth hormone release was reduced at as early as 1 h after SRIF exposure. SRIF did not have a significant effect on total prolactin content or prolactin mRNA levels, suggesting the absence of an effect on prolactin synthesis. No increase in the magnitude of the inhibitory effect of SRIF was observed when using pituitary cells from immature, mature male or mature female trout. When comparing effects on primary cultures containing cells from the whole pituitary with a prolactin cell-enriched population, SRIF appeared to have the same inhibitory effect on prolactin release, supporting a direct action of SRIF on prolactin cells. These results provide further support for SRIF being a prolactin-inhibiting factor in rainbow trout and acting as a modulator of a dominant stimulatory control of prolactin release.

show abstract

Section: Discussionsupporting

confidence: 87%

Effect of somatostatin on prolactin in rainbow trout (Oncorhynchus mykiss) pituitary cells in primary culture

Goff¹,

Weil²,

Valotaire³

et al. 1992

Journal of Molecular Endocrinology

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“…The localization to a particular site could help the hypothalamus to innervate prolactin cells and regulate their functions. While dopamine receptors in prolactin cells inhibit prolactin release in both mammals and teleosts [7,18], teleostean hypothalamic prolactin releasing factor (PRF) plays a more prominent role than that of mammals [19]. Dopamine not only acts on D 2 -receptors in prolactin cells, but also stimulates GH secretion through D 1 -like-receptors [20] and modulates GTH secretion in teleosts [21,22].…”

Section: Discussionmentioning

confidence: 99%

Dopamine causes ultrastructural changes in prolactin cells of tilapia ( Oreochromis niloticus )

Hazineh

Shin

Reifel

et al. 1997

Cellular and Molecular Life Sciences (CMLS)

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This study was undertaken to examine ultrastructural changes induced by dopamine in fish prolactin cells. Tilapia adenohypophyses were incubated with dopamine and evaluated by electron microscopy. The quantities of rough endoplasmic reticulum (RER) in prolactin cells increased and the number of secretory granules were decreased by dopamine (10(-6) mol/l) treatment. Another set of adenohypophysial tissues was placed back into control medium for 10 min following a 3 h incubation period with dopamine (10(-6) mol/l) (RE10 min group). This group had significantly less RER than the 3 h dopamine-treated tissue, and the shape of many granules in the RE10 min group changed from spherical to rod-like. In addition, some of the granule content appeared to diffuse out of granules since some were not fully surrounded by membrane. It was therefore hypothesized that the rod-shaped granules might be the result of prolactin secretion by diffusion.

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Effects of osmotic pressure on prolactin and growth hormone secretion from organ-cultured eel pituitary

1991

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Hypothalamic control of prolactin release in the rainbow trout,Salmo gairdneri: in vitro studies

Cited by 7 publications

References 37 publications

Effect of somatostatin on prolactin in rainbow trout (Oncorhynchus mykiss) pituitary cells in primary culture

Effect of somatostatin on prolactin in rainbow trout (Oncorhynchus mykiss) pituitary cells in primary culture

Dopamine causes ultrastructural changes in prolactin cells of tilapia ( Oreochromis niloticus )

Effects of osmotic pressure on prolactin and growth hormone secretion from organ-cultured eel pituitary

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