Hypothalamic long noncoding RNA AK044061 is involved in the development of dietary obesity in mice

Li, Juan; Long, Jinlie; Zhang, Qiong; Shen, Hongyuan; Guo, An‐Yuan; Ma, Zhaowu; Zhang, Guo

doi:10.1038/s41366-021-00945-9

Cited by 9 publications

(8 citation statements)

References 50 publications

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“…To better estimate the abundance of lncRNAs, we merged the lncRNA annotations files of Ensembl version 84 and NONCODE version 6.0 by GffCompare (version 0.9.8) as previously described. 13 In brief, class codes from the output of GffCompare were used to explore relationships between lncRNAs of NONCODE and genes of Ensembl: (1) lncRNAs of NONCODE with class codes of “=” and “c” were deemed redundant transcripts from Ensembl, and their NONCODE IDs were replaced by Ensembl ones (their expression will be merged with Ensembl genes); (2) lncRNAs with other types of class codes were considered novel lncRNAs compared with those found in the Ensembl annotation. In addition, we inspected flanking genes around lncRNAs via a sliding window using bedtools (version 2.17).…”

Section: Methodsmentioning

confidence: 99%

A comprehensive landscape of transcription profiles and data resources for human leukemia

et al. 2023

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The tyrosine kinase inhibitor dasatinib is approved for Philadelphia chromosome positive leukemia, including chronic myeloid leukemia (CML). While effective and well tolerated, patients typically exhibit a transient lymphocytosis following dasatinib uptake. The underlying physiological process linking dasatinib to lymphocytosis has remained unknown to date. Here, we used a small rodent model to examine the mechanism of dasatinib-induced lymphocytosis, focusing on lymphocyte trafficking into and out of secondary lymphoid organs (SLO). Our data indicate that lymphocyte homing to lymph nodes (LN) and spleen remained unaffected by dasatinib treatment. In contrast, dasatinib promoted lymphocyte egress from spleen with kinetics consistent with the observed lymphocytosis. Unexpectedly, dasatinib-induced lymphocyte egress occurred independently of canonical sphingosine-1-phosphate-mediated egress signals. Instead, dasatinib treatment led to a decrease in spleen size, concomitant with increased splenic stromal cell contractility as measured by myosin light chain phosphorylation. Accordingly, dasatinib-induced lymphocytosis was partially reversed by pharmacological inhibition of the contraction-promoting factor ROCK. Finally, we uncovered a decrease in spleen size of CML patients who showed lymphocytosis immediately following dasatinib treatment, and this reduction was proportional to the magnitude of lymphocytosis and dasatinib plasma levels. In sum, our work provides evidence that dasatinib-induced lymphocytosis is a consequence of drug-induced contractility of splenic stromal cells.

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Section: Methodsmentioning

confidence: 99%

A comprehensive landscape of transcription profiles and data resources for human leukemia

et al. 2023

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“…Differential expression analysis of RNA-seq data was performed as described in our previous study. [78] Statistics: Body weight data were analyzed using two-tailed Student's t-test. Unless otherwise noted, a p value less than 0.05 was considered statistically significant.…”

Section: Methodsmentioning

confidence: 99%

“…Differential expression analysis of RNA‐seq data was performed as described in our previous study. [ 78 ]…”

Section: Methodsmentioning

confidence: 99%

The Hypothalamic Epigenetic Landscape in Dietary Obesity

Ma,

Yin,

et al. 2023

Advanced Science

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The hypothalamus in the brain plays a pivotal role in controlling energy balance in vertebrates. Nutritional excess through high‐fat diet (HFD) feeding can dysregulate hypothalamic signaling at multiple levels. Yet, it remains largely unknown in what magnitude HFD feeding may impact epigenetics in this brain region. Here, it is shown that HFD feeding can significantly alter hypothalamic epigenetic events, including posttranslational histone modifications, DNA methylation, and chromatin accessibility. The authors comprehensively analyze the chromatin immunoprecipitation‐sequencing (ChIP‐seq), methylated DNA immunoprecipitation‐sequencing (MeDIP‐seq), single nucleus assay for transposase‐accessible chromatin using sequencing (snATAC‐seq), and RNA‐seq data of the hypothalamus of C57 BL/6 mice fed with a chow or HFD for 1 to 6 months. The chromatins are categorized into 6 states using the obtained ChIP‐seq data for H3K4me3, H3K27ac, H3K9me3, H3K27me3, and H3K36me3. A 1‐month HFD feeding dysregulates histone modifications and DNA methylation more pronouncedly than that of 3‐ or 6‐month. Besides, HFD feeding differentially impacts chromatin accessibility in hypothalamic cells. Thus, the epigenetic landscape is dysregulated in the hypothalamus of dietary obesity mice.

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“…LncRNAs are the transcription products of non-protein-coding segments of the genome with a length of more than 200 nucleotides. [75][76][77][78][79] According to the data from LNCipedia (accessed on 14th March 2023) reported 127,802 transcripts and 56,946 genes. 80 LncRNAs regulate gene expression by various mechanisms.…”

Section: Long Noncoding Rnasmentioning

confidence: 99%

Noncoding RNAs as regulators of STAT3 pathway in gastrointestinal cancers: Roles in cancer progression and therapeutic response

Ashrafizadeh

Mohan

Rangappa

et al. 2023

Medicinal Research Reviews

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Gastrointestinal (GI) tumors (cancers of the esophagus, gastric, liver, pancreas, colon, and rectum) contribute to a large number of deaths worldwide. STAT3 is an oncogenic transcription factor that promotes the transcription of genes associated with proliferation, antiapoptosis, survival, and metastasis. STAT3 is overactivated in many human malignancies including GI tumors which accelerates tumor progression, metastasis, and drug resistance. Research in recent years demonstrated that noncoding RNAs (ncRNAs) play a major role in the regulation of many signaling pathways including the STAT3 pathway. The major types of endogenous ncRNAs that are being extensively studied in oncology are microRNAs, long noncoding RNAs, and circular RNAs. These ncRNAs can either be tumor‐promoters or tumor‐suppressors and each one of them imparts their activity via different mechanisms. The STAT3 pathway is also tightly modulated by ncRNAs. In this article, we have elaborated on the tumor‐promoting role of STAT3 signaling in GI tumors. Subsequently, we have comprehensively discussed the oncogenic as well as tumor suppressor functions and mechanism of action of ncRNAs that are known to modulate STAT3 signaling in GI cancers.

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Hypothalamic long noncoding RNA AK044061 is involved in the development of dietary obesity in mice

Cited by 9 publications

References 50 publications

A comprehensive landscape of transcription profiles and data resources for human leukemia

A comprehensive landscape of transcription profiles and data resources for human leukemia

The Hypothalamic Epigenetic Landscape in Dietary Obesity

Noncoding RNAs as regulators of STAT3 pathway in gastrointestinal cancers: Roles in cancer progression and therapeutic response

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