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The reference intervals of complete blood count (CBC) parameters were commonly based on healthy individuals aged 20 to 79 years. However, these values are not optimal for correct clinical diagnosis in older individuals (e.g., 80-89 years). Although the reference intervals for this age group have been reported in China, there is no population-based report in Guizhou province. A total of 481 healthy adults (238 males and 243 females) aged 80 to 89 years were recruited from Affiliated Hospital of Zunyi Medical University in Guizhou. The CBC parameters were detected by Sysmex XN-9000 automatic hematology analyzer. The reference intervals of the components were analyzed according to the guidelines of International Federation of Clinical Chemistry. This study reported the reference intervals of CBC parameters. There were significant differences were examined in some reference intervals between the different gender groups, especially for RBC-related parameters. Compared with national standards, the most of all conventional reference intervals for CBC parameters were decreased. The present study provided the local reference intervals of CBC parameters for individuals aged 80 to 89 years in Guizhou, China. Some of our results were sex-specific, and most of our results show lower values while comparing with commonly used reference intervals in China. Therefore, more attentions should be paid to these differences, and accurate reference intervals will facilitate clinical diagnosis and decision-making in these populations.

Smile aesthetics are mainly influenced by the relative position of facial midline (FC-line), anterior tooth midline (AT-line) and labial tubercle midline (LT-line). However, the acceptable deviation of LT-line and AT-line relative to FC-line is unknown. This study aims to fill the critical gap. We adopted the method of cross-sectional study, the frontal full-face smile photographs of 1 subject set (1 male) were enrolled. Taking the FC-line as the center line, twelve images with 1-mm increment relative to FC-line (right or left deviation, the maximum deviation distance was 3-mm) in LT-line deviation model or LT + AT-line deviation model (LT-line coincided with AT-line basing on LT-line deviation model) were produced. Basing on Q-sort assessment, the images were evaluated by 160 dentists, 165 orthodontic patients and 163 freshmen. And the collected Q-sort scores were subjected to nonparametric comparative analysis using SPSS 18.0. There were significant differences in Q-sort scores among different groups ( P < .01). When the deviation distance was 1 mm in dentist and orthodontic patient or 2 mm in freshman group, there was no significant difference in smile attractiveness scores between the LT line deviation model and the LT + AT line deviation model ( P > .05). We also found that the score of male dentist significantly higher than female dentist ( P < .05). However, the scores of right deviation in dentists and orthodontic patients were significantly lower than those in left deviation ( P < .05). The acceptable deviation of LT-line and AT-line relative to FC-line should be kept within 2 mm. Besides, raters’ occupation and gender, and deviation direction of model may influence the smile aesthetics.