Hypothalamic-pituitary-adrenocortical and gonadal axes and sympathoadrenal activity of adult male rats prenatally exposed to morphine

Dutriez-Casteloot, Isabelle; Bernet, François; Dedieu, Jean-François; Croix, Dominique; Laborie, Christine; Montel, Valérie; Lésage, Jean; Beauvillain, Jean-Claudé; Dupouy, Jean‐Paul

doi:10.1016/s0304-3940(99)00086-5

Cited by 22 publications

(21 citation statements)

References 24 publications

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“…The unchanged basal plasma ACTH and CORT levels agree with our previous results in male rats [2]as well as with the works of Dutriez-Casteloot et al and Lesage et al [21, 22]. However, while Dutriez-Casteloot et al [8]showed prenatal morphine exposure-induced differences in ACTH and CORT levels in some stages of the estrous cycle, we did not see such differences in morphine-exposed females in the present study.…”

Section: Discussionsupporting

confidence: 92%

Hypothalamo-Pituitary-Adrenal Axis-Regulated Stress Response and Negative Feedback Sensitivity Is Altered by Prenatal Morphine Exposure in Adult Female Rats

et al. 2004

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It has been shown that adult female rats react to stressors more intensely than adult male rats. Our previous work demonstrated that the adrenocorticotropic hormone (ACTH) but not corticosterone (CORT) response to stress is altered by prenatal morphine exposure in adult male rats. Response of the hypothalamo-pituitary-adrenal (HPA) axis to stress is known to be sex specific and dependent on the hormonal fluctuation of the estrous cycle. Therefore, the present study examined the effect of prenatal morphine exposure on the levels of ACTH and CORT before and after restraint stress in adult female rats. Experiment 1 tested ACTH and CORT plasma levels before and after restraint stress in prenatally morphine- and saline-exposed, adult diestrus and proestrus female rats. Prenatal morphine exposure suppressed the restraint stress-induced ACTH levels in both diestrus and proestrus females, but did not have any effects on the basal or stress-induced CORT levels. Experiment 2 examined the sensitivity of negative feedback using the dexamethasone (DEX) (0.001, 0.01, 0.1 and 1.0 mg/kg) suppression test in adult, prenatally morphine- and saline-exposed female rats. In saline-exposed, proestrus but not diestrus females, all doses of DEX were effective in suppressing the restraint stress-induced increase in CORT levels. In both diestrus and proestrus, morphine-exposed females, only the two highest doses of DEX (0.1 and 1.0 mg/kg) were successfully suppressing the stress-induced CORT levels. The stress-induced increase in the ACTH level was suppressed only by the highest dose of DEX (1.0 mg/kg) in both saline- and morphine-exposed, diestrus and proestrus females. Thus, the present study demonstrates that prenatal morphine exposure alters the HPA axis-regulated stress response and the sensitivity of negative feedback that are affected by the fluctuation of ovarian hormones.

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Section: Discussionsupporting

confidence: 92%

Hypothalamo-Pituitary-Adrenal Axis-Regulated Stress Response and Negative Feedback Sensitivity Is Altered by Prenatal Morphine Exposure in Adult Female Rats

et al. 2004

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“…Lesage et al [7, 20, 21, 22]found that prenatal morphine exposure induces adrenal atrophy, hypoactivity of the glucocorticoid function, and hyperactivity of mineralocorticoid function in newborns. It was suggested [20, 22]that this effect of prenatal morphine exposure may be due to an altered HPA axis.…”

Section: Introductionmentioning

confidence: 99%

“…Wotus et al [52]showed that by embryonic day 16, the adrenals are already differentiated and producing aldosterone and CORT. Interestingly, Lesage et al [7]demonstrated that in adult male rats, prenatal morphine exposure does not alter hypothalamic CRH content, ACTH and CORT levels, or adrenal weight, nor does it alter the B max and K d of mineralocorticoid and glucocorticoid receptors in the hippocampus and hypothalamus. Thus, it seems that the effect of prenatal morphine exposure does not last until adulthood.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it seems that the effect of prenatal morphine exposure does not last until adulthood. However, they [7]examined only basal levels of pituitary and adrenal hormones without considering hormone responses to stress. It is therefore possible that while the basal level of CORT remains unchanged in prenatally morphine-exposed, adult males [7], the response of the HPA axis to stressor(s) might be altered.…”

Section: Introductionmentioning

confidence: 99%

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Adrenocorticotropin Stress Response but Not Glucocorticoid-Negative Feedback Is Altered by Prenatal Morphine Exposure in Adult Male Rats

et al. 2003

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The present study was designed to investigate the effects of prenatal morphine exposure on the hypothalamic-pituitary-adrenal (HPA) axis-regulated stress responses by measuring restraint stress-induced changes in the adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels. In experiment 1, plasma levels of ACTH and CORT in prenatally morphine-, saline-exposed and control male rats were determined before and at several times after restraint stress. There were no statistically significant differences in plasma ACTH and CORT levels before restraint stress between the groups. However, prenatal morphine exposure dampened the stress-induced increase and spontaneous recovery of ACTH levels after the restraint stress. There were no differences in plasma CORT levels between the three groups either before or at any time after restraint stress. Experiment 2 was designed to investigate the sensitivity of negative feedback of glucocorticoids using the dexamethasone (DEX) suppression test. DEX was administered at different doses (0.001, 0.01, 0.1 and 1.0 mg/kg) and ACTH and CORT plasma levels were measured before and at several times after restraint stress in prenatally morphine- and saline-exposed males. DEX pretreatment eliminated the differences observed in ACTH responses to stress in morphine- and saline-exposed males. DEX pretreatment dose dependently suppressed the restraint stress-induced increased plasma ACTH concentration. In plasma CORT levels, DEX pretreatment dose dependently suppressed the restraint stress-induced increased plasma CORT concentration regardless of prenatal drug exposure. Thus, the present study demonstrates that prenatal morphine exposure alters the ACTH and CORT responses to stress but not the sensitivity of negative feedback of glucocorticoids.

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“…Outros trabalhos mostram que a P 4 não tem efeito sobre a atividade do eixo HPA em condição basal ou de estresse (CAREY et al , 1995;YOUNG et al , 2001). PATCHEV, ALMEIDA, 1996;DUTRIEZ-CASTELOOT et al , 1999;YOUNG et al , 2001).…”

Section: -Ação Dos Esteróides Ovarianos Sobre a Secreção De Cort E Acthunclassified

Efeitos de estrógeno e de progesterona na atividade basal do eixo hipotálamo hipófise adrenal

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Hypothalamic-pituitary-adrenocortical and gonadal axes and sympathoadrenal activity of adult male rats prenatally exposed to morphine

Cited by 22 publications

References 24 publications

Hypothalamo-Pituitary-Adrenal Axis-Regulated Stress Response and Negative Feedback Sensitivity Is Altered by Prenatal Morphine Exposure in Adult Female Rats

Hypothalamo-Pituitary-Adrenal Axis-Regulated Stress Response and Negative Feedback Sensitivity Is Altered by Prenatal Morphine Exposure in Adult Female Rats

Adrenocorticotropin Stress Response but Not Glucocorticoid-Negative Feedback Is Altered by Prenatal Morphine Exposure in Adult Male Rats

Efeitos de estrógeno e de progesterona na atividade basal do eixo hipotálamo hipófise adrenal

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