Hypothalamic Proline-Rich Peptide-1 Protects Against Myocardial Ischemia-Reperfusion Injury

Abstract

Hypothalamic proline-rich peptide-1 (PRP-1), a neurosecretory cytokine appeared to be involved in the multiple mechanisms of cardioprotection. Dose-dependent effects of PRP-1 (4 and 8 µg) were studied in an open-chest model of myocardial ischemia-reperfusion injury (IRI). Adult male Sprague-Dawley rats underwent 40-minute left anterior descending coronary artery occlusion, under isoflurane anesthesia followed by 2 and/or 24 h of reperfusion. Groups treated with PRP-1 were compared to control. It has been revealed that the efficient dose of PRP-1 can restore in a time-dependent manner the contractile activity of the myocardium and suppress the both inflammation and necrosis via amelioration of oxidative stress in the cardiac tissues therethrough contributing to the in vivo reduction of myocardial infarct volume and an improvement the cardiac hemodynamics and coronary circulation. New studies are needed to ascertain a beneficial effect of PRP-1 in humans and its future clinic use for the myocardial IRI treatment

The effect of hypothalamic peptides, neurohormone C and proline-rich peptide-1on the Ca2+-handling system in heartin pathophysiological conditions

“…Open-chest model of myocardial IR injury is the most acceptable and suitable model for the reproduction of human MI and screening of pharmacological activity of various compounds [56,57]. Using this model, we demonstrated that post-ischemic administration of an effective dose of PRP-1 significantly reduces in vivo necrotic area of myocardial infarction, caused by temporary occlusion of coronary artery in rats, as well as it improves cardiac hemodynamics and coronary circulation, by increasing the left ventricular ejection fraction, which is an indicator of efficiency of pumping into the systemic circulation [58].…”