Hypothermia-mediated oxidative stress induces immunosuppression, morphological impairment and cell fate disorder in the intestine of freshwater drum, Aplodinotus grunniens

Chen, Jianxiang; Xu, Pao; Huang, Wen; Xue, Miaomiao; Wang, Qingyong; He, Jun; He, Cijiang; Su, Shengyan; Li, Jianlin; Fan, Yuding; Feng, Wenrong; Wang, Meiyao; Song, Changyou; Li, Hongxia

doi:10.1016/j.aquaculture.2023.739805

Cited by 8 publications

(7 citation statements)

References 48 publications

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“…All animal procedures were performed in accordance with the Guideline for the Care and Use of Laboratory Animals in China. The ethical situation in this experiment was identical to that in our previously published paper [ 26 ].…”

Section: Methodsmentioning

confidence: 85%

“…The results showed that HFD feeding reduced immunoglobulin (IgG and IgM) levels. In addition, heat shock proteins (HSPs) are involved in cellular antioxidant and innate immune responses [ 26 ]. Transcript levels of HSP70 and HSP90 were dramatically decreased under HFD feeding, which may be attributable to oxidative stress caused by HFD as well as suppression of antioxidant capacity, which further compromises the gut immune system.…”

Section: Discussionmentioning

confidence: 99%

“…This may further support the fact that excessive lipid intake causes oxidative stress through increased ROS levels which in turn leads to oxidative damage. To maintain normal physiological functions, the organism removes unnecessary or abnormal cells through apoptosis [ 26 ]. We discovered that an HFD activates apoptotic genes ( Casp8 , Casp3 , Bax , Bcl2 ) in the gut, demonstrating that apoptosis is a key response mechanism for maintaining homeostasis in the body under a high-fat diet.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we introduced freshwater drum and conducted a great deal of related research. We conducted feed domestication and discovered potential regulatory mechanisms in freshwater drum [ 25 ], explored the mechanisms of intestinal adaptation to hypothermia [ 26 ] and identified the role of miR-1/AMPK in the regulation of glycolipid metabolism [ 24 ] as well as the role of the PPAR signaling pathway in the hypothermic regulation of lipid and amino acid metabolic homeostasis [ 27 ]. The above studies found that freshwater drum are suitable for nationwide culture and promotion.…”

Section: Introductionmentioning

confidence: 99%

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A High-Fat-Diet-Induced Microbiota Imbalance Correlates with Oxidative Stress and the Inflammatory Response in the Gut of Freshwater Drum (Aplodinotus grunniens)

Xue,

Xu,

Wen

et al. 2024

Antioxidants

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Lipids are critical nutrients for aquatic animals, and excessive or insufficient lipid intake can lead to physiological disorders, which further affect fish growth and health. In aquatic animals, the gut microbiota has an important regulatory role in lipid metabolism. However, the effects of a high-fat diet on physical health and microbiota diversity in the gut of freshwater drum (Aplodinotus grunniens) are unclear. Therefore, in the present study, a control group (Con, 6%) and a high-fat diet group (HFD, 12%) were established for a 16-week feeding experiment in freshwater drum to explore the physiological changes in the gut and the potential regulatory mechanisms of bacteria. The results indicated that a high-fat diet inhibited antioxidant and immune capacity while increasing inflammation, apoptosis and autophagy in gut cells. Transcriptome analysis revealed significant enrichment in immune-related, apoptosis-related and disease-related pathways. Through 16S rRNA analysis, a total of 31 genus-level differentially abundant bacterial taxa were identified. In addition, a high-fat diet reduced gut microbial diversity and disrupted the ecological balance of the gut microbiota (Ace, Chao, Shannon and Simpson indices). Integrated analysis of the gut microbiota combined with physiological indicators and the transcriptome revealed that gut microbial disorders were associated with intestinal antioxidants, immune and inflammatory responses, cell apoptosis and autophagy. Specifically, genus-level bacterial taxa in Proteobacteria (Plesiomonas, Arenimonas, Erythrobacter and Aquabacteriumb) could serve as potential targets controlling the response to high-fat-diet stimulation.

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Section: Methodsmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A High-Fat-Diet-Induced Microbiota Imbalance Correlates with Oxidative Stress and the Inflammatory Response in the Gut of Freshwater Drum (Aplodinotus grunniens)

Xue,

Xu,

Wen

et al. 2024

Antioxidants

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“…Increased levels of antioxidant components in the liver are a crucial part of the oxidative stress response. Winter photoperiod exposure induced oxidative stress in the liver of the Huanghe carp with a significant increase in MDA, which is a product of lipid peroxidation [34]. GSH-Px, an important peroxidative catabolic enzyme, showed significantly higher activity in carp exposed to the winter photoperiod as compared to the control group, probably due to the effect of winter photoperiod exposure on the cellular function of carp, which synthesizes a large amount of GSH-PX to protect cellular structure and function from the effects and damage of oxidants [35].…”

Section: Discussionmentioning

confidence: 99%

Effects of Seasonal Photoperiod on Growth, Lipid Metabolism, and Antioxidant Response in the Huanghe Carp (Cyprinus carpio haematopterus)

Wang,

Su,

Dong

et al. 2023

Fishes

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Photoperiod is one of the most important environmental cues for organisms, and it plays a crucial role in regulating feeding, behavior, growth, and metabolism. However, seasonal photoperiods are often overlooked in carp culture or experiments, with a poorly understood effect on lipid metabolism and oxidative stress in fish. To explore the effects of seasonal photoperiods, we exposed Huanghe carp (Cyprinus carpio haematopterus) to summer photoperiod (14 h light:10 h dark) and winter photoperiod (10 h light:14 h dark) daylight conditions in an eight-week experiment. Our results suggested that the winter photoperiod significantly increased the liver TG level as well as the transcript levels of genes related to lipid synthesis, indicating that the lipid metabolism in Huanghe carp liver was enhanced compared to summer photoperiod conditions, and that lipid deposition may be responsible for the increase in body weight level and hepatosomatic index. Additionally, MDA, GSH, GSH-PX, and T-AOC levels were significantly elevated in the liver of fish under the winter photoperiod, suggesting that Huanghe carp responded to winter photoperiod exposure-induced oxidative stress in the liver by enhancing the antioxidant response. Based on transcriptome analysis, the winter photoperiod activated hepatic autophagy response and the FOXO signaling pathway in Huanghe carp. Combined with the correlation analysis, the Huanghe carp maintains the physiological health of the liver by activating the FOXO signaling pathway-mediated cell cycle regulation and autophagy response in response to oxidative stress during winter photoperiod exposure. Our study provides the first evidence for the physiological regulation of the liver in Huanghe carp under seasonal photoperiod stimulation.

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Fattening with formulated diets improves the immune response and lipid accumulation in the gonads of male Chinese mitten crab (Eriocheir sinensis)

Chen,

Xu,

et al. 2025

Aquaculture

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Hypothermia-mediated oxidative stress induces immunosuppression, morphological impairment and cell fate disorder in the intestine of freshwater drum, Aplodinotus grunniens

Cited by 8 publications

References 48 publications

A High-Fat-Diet-Induced Microbiota Imbalance Correlates with Oxidative Stress and the Inflammatory Response in the Gut of Freshwater Drum (Aplodinotus grunniens)

A High-Fat-Diet-Induced Microbiota Imbalance Correlates with Oxidative Stress and the Inflammatory Response in the Gut of Freshwater Drum (Aplodinotus grunniens)

Effects of Seasonal Photoperiod on Growth, Lipid Metabolism, and Antioxidant Response in the Huanghe Carp (Cyprinus carpio haematopterus)

Fattening with formulated diets improves the immune response and lipid accumulation in the gonads of male Chinese mitten crab (Eriocheir sinensis)

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