Hypothermic storage of sheep embryos with antifreeze proteins: Development in vitro and in vivo

Baguisi, A.; Arav, Amir; Crosby, T.F.; Roche, J. F.; Boland, M.P.

doi:10.1016/s0093-691x(97)00328-2

Cited by 38 publications

(18 citation statements)

References 23 publications

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“…The most commonly used basal medium for hypothermic preservation of bovine and sheep embryos is PBS162829. We examined effect of FBS on preservation when PBS, medium 199, and L15 were used as basal media.…”

Section: Discussionmentioning

confidence: 99%

A simple medium enables bovine embryos to be held for seven days at 4°C

Ideta

Aoyagi

Tsuchiya

et al. 2013

Sci Rep

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Cryopreservation methods using liquid nitrogen (LN2) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN2 containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN2-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days.

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Section: Discussionmentioning

confidence: 99%

A simple medium enables bovine embryos to be held for seven days at 4°C

Ideta

Aoyagi

Tsuchiya

et al. 2013

Sci Rep

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“…Embryos from fish [166,186,187,196,202], cows [172,203], sheep [175], rabbits [176], mice [67,92,183,191], and horses [91] were preserved in the presence of AFPs. Early attempts with equine and mouse embryos demonstrated that fish AFPs had negligible effects [91,92]; however, fish embryos subjected to microinjection or incubation in type I AFP solution showed significantly increased survival after chilling at 4 °C or −10 °C.…”

Section: Cryopreservation Using Afps As a Potential Cryoprotectantmentioning

confidence: 99%

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

et al. 2017

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Antifreeze proteins (AFPs) are biological antifreezes with unique properties, including thermal hysteresis (TH), ice recrystallization inhibition (IRI), and interaction with membranes and/or membrane proteins. These properties have been utilized in the preservation of biological samples at low temperatures. Here, we review the structure and function of marine-derived AFPs, including moderately active fish AFPs and hyperactive polar AFPs. We also survey previous and current reports of cryopreservation using AFPs. Cryopreserved biological samples are relatively diverse ranging from diatoms and reproductive cells to embryos and organs. Cryopreserved biological samples mainly originate from mammals. Most cryopreservation trials using marine-derived AFPs have demonstrated that addition of AFPs can improve post-thaw viability regardless of freezing method (slow-freezing or vitrification), storage temperature, and types of biological sample type.

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“…Related studies on the influence of TTTT and BSA on the storage of ovine embryos at both 4 °C and 0 °C for periods of up to 4 days, showed pregnancy rates similar to those for fresh embryos when embryos were stored at 4 °C for 4 days in the presence of TTTT [65]. While there was no discussion as to the mechanism(s) by which TTTT mediates this protective function, the fact that BSA which has no antifreeze activity, gave the same results, raises the important question of whether protective properties are specific to the AFPs.…”

Section: Studies With Biological Samplesmentioning

confidence: 79%

Applications of Type I Antifreeze Proteins: Studies with Model Membranes & Cryoprotectant Properties

Inglis¹,

Turner²,

Harding³

2006

CPPS

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Antifreeze proteins (AFPs) and antifreeze glycoproteins (AFGPs), found in the body fluids of many species of polar fish allow them to survive in waters colder than the equilibrium freezing point of their blood and other internal fluids. Despite their structural diversity, all AF(G)Ps kinetically depress the temperature at which ice grows in a non-colligative manner and hence exhibit thermal hysteresis. AF(G)Ps also share the ability to interact with and protect mammalian cells and tissues from hypothermic damage (e.g., improved storage of human blood platelets at low temperatures), and are able to stabilize or disrupt membrane composition during low temperature and freezing stress (e.g., cryoprotectant properties in stabilization of sperm and oocytes). This review will summarize studies of AFPs with phospholipids and plant lipids, proposed mechanisms for inhibition of leakage from membranes, and cryoprotectant studies with biological samples. The major focus will be on the alpha-helical type I antifreeze proteins, and synthetic mutants, that have been most widely studied. For completeness, data on glycoproteins will also be presented. While a number of models to explain stabilization and destabilization of different lipid systems have been proposed, it is currently not possible to predict whether a particular AFP will stabilize or destabilize a given lipid system. Furthermore the relationship between the antifreeze property of thermal hysteresis and membrane stabilization is unknown. This lack of detailed knowledge about how AFPs function in the presence of different types of materials has hampered progress toward the development of antifreezes for cold storage of cells, tissues, and organs.

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Hypothermic storage of sheep embryos with antifreeze proteins: Development in vitro and in vivo

Cited by 38 publications

References 23 publications

A simple medium enables bovine embryos to be held for seven days at 4°C

A simple medium enables bovine embryos to be held for seven days at 4°C

Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant

Applications of Type I Antifreeze Proteins: Studies with Model Membranes & Cryoprotectant Properties

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