Hypoxia induced HIF-1/HIF-2 activity alters trophoblast transcriptional regulation and promotes invasion

Highet, Amanda R.; Khoda, Sultana Mahabbat-e; Buckberry, Sam; Leemaqz, Shalem; Bianco‐Miotto, Tina; Harrington, Elaine; Ricciardelli, Carmela; Roberts, Claire T.

doi:10.1016/j.ejcb.2015.10.004

Cited by 63 publications

(46 citation statements)

References 82 publications

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“…34 In addition, concomitant increases in IGFBP3 and invasion have been observed in HTR8/SVneo cells under hypoxic conditions. 35 Together, IGFBP3 displays the same localization in placenta and possible function on HTR8/SVneo cell invasion as ID1. IGFBP3 also promotes endothelial precursor cell differentiation, migration and capillary-like tube formation, suggesting similar effects as BMP2 and ID1 on angiogenesis.…”

Section: Introductionmentioning

confidence: 95%

“…Likewise, whole genome microarray analysis showed that IGFBP3 mRNA levels are significantly higher in EVTs than in cytotrophoblasts . In addition, concomitant increases in IGFBP3 and invasion have been observed in HTR8/SVneo cells under hypoxic conditions . Together, IGFBP3 displays the same localization in placenta and possible function on HTR8/SVneo cell invasion as ID1.…”

Section: Introductionmentioning

confidence: 97%

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Bone morphogenetic protein 2 promotes human trophoblast cell invasion and endothelial‐like tube formation through ID1‐mediated upregulation of IGF binding protein‐3

et al. 2020

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Bone morphogenetic protein 2 promotes human trophoblast cell invasion and endothelial‐like tube formation through ID1‐mediated upregulation of IGF binding protein‐3

et al. 2020

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“…Data from mouse (Heras et al 2016) and human (Highet et al 2015, Kleijkers et al 2015 studies using different transcriptomic platforms are also provided. The transcriptomic biomarkers associated with compromised preimplantation development after IVC are involved in important biological functions such as metabolic homeostasis, extracellular matrix remodelling, inflammation and signal transduction.…”

Section: Common Transcriptomic Manifestations Of Ivc Stress In Low-qumentioning

confidence: 99%

The embryonic stress response to in vitro culture: insight from genomic analysis

Cagnone

Sirard

2016

Reproduction

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Recent genomic studies have shed light on the impact of in vitro culture (IVC) on embryonic homeostasis and the differential gene expression profiles associated with lower developmental competence. Consistently, the embryonic stress responses to IVC conditions correlate with transcriptomic changes in pathways related to energetic metabolism, extracellular matrix remodelling and inflammatory signalling. These changes appear to result from a developmental adaptation that enhances a Warburg-like effect known to occur naturally during blastulation. First discovered in cancer cells, the Warburg effect (increased glycolysis under aerobic conditions) is thought to result from mitochondrial dysfunction. In the case of IVC embryos, culture conditions may interfere with mitochondrial maturation and oxidative phosphorylation, forcing cells to rely on glycolysis in order to maintain energetic homeostasis. While beneficial in the short term, such adaptations may lead to epigenetic changes with potential long-term effects on implantation, foetal growth and post-natal health. We conclude that lessening the detrimental effects of IVC on mitochondrial activity would lead to significantly improved embryo quality.Reproduction (2016) 152 R247-R261

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“…RNA isolation and clean-up, labelling and hybridisation to Illumina Human HT-12 v4 Gene expression arrays has been published previously (Highet et al, 2015). The probe-level background-corrected raw data output was variance stabilised , log2 transformed, quantile normalised and quality checked using the Lumi package .…”

Section: Gene Expression Microarraymentioning

confidence: 99%

“…Cells were cultured with Matrigel (HTR8-M) or without Matrigel™ on plastic culture surface (HTR8-P) as previously described (Highet et al, 2015) and incubated in 21% oxygen for 6 h.…”

Section: Cell Culturementioning

confidence: 99%

First trimester trophoblasts forming endothelial-like tubes in vitro emulate a ‘blood vessel development’ gene expression profile

Highet

Buckberry

Mayne

et al. 2016

Gene Expression Patterns

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Hypoxia induced HIF-1/HIF-2 activity alters trophoblast transcriptional regulation and promotes invasion

Cited by 63 publications

References 82 publications

Bone morphogenetic protein 2 promotes human trophoblast cell invasion and endothelial‐like tube formation through ID1‐mediated upregulation of IGF binding protein‐3

Bone morphogenetic protein 2 promotes human trophoblast cell invasion and endothelial‐like tube formation through ID1‐mediated upregulation of IGF binding protein‐3

The embryonic stress response to in vitro culture: insight from genomic analysis

First trimester trophoblasts forming endothelial-like tubes in vitro emulate a ‘blood vessel development’ gene expression profile

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