Hypoxia‐inducible factor‐1α inhibits interleukin‐6 and ‐8 production in gingival epithelial cells during hypoxia

Takedachi, Masahide; Iyama, Mitsuyoshi; Sawada, Kaichiro; Mori, Kensaku; Yamamoto, Shozo; Morimoto, Chiaki; Yanagita, Manabu; Murakami, Shinya

doi:10.1111/jre.12377

Cited by 18 publications

(11 citation statements)

References 38 publications

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“…According to a previous study (Takedachi et al 2017), in our post mortem samples, HIF-1a immunostaining was higher in the oral epithelium than it was in the sub-oral connective tissues, thus reflecting the in vivo distribution observed in mice under hypoxic-inflammatory conditions. Interestingly, as shown in the epithelial cells in a rat model, in which the level of HIF-1a expression was relatively higher in the suprabasal layer, our results indicated a high staining reactivity for HIF-1a that was detectable in the deeper layers of the oral mucosa and decreased gradually in the more superficial layers.…”

Section: Discussionsupporting

confidence: 79%

“…Hydroxylation by the prolyl-4-hydroxylases (PHDs) or HIF-1 prolyl hydroxylases of one of two critical proline residues in their oxygen-dependent degradation domain mediates their interaction with the von Hippel-Lindau (VHL) E3 ubiquitin ligase complex that targets them for rapid proteasomal degradation (Bruick & McKnight, 2001;Hirsil€ a et al 2005;Ng et al 2011;Masoud & Lin, 2015). Because the hydroxylation action from PHDs requires the presence of oxygen, a hypoxic environment will inhibit the activities of the HIF-P4Hs such that HIF-1a escapes degradation, translocates to the nucleus and binds HIF-b; this dimer then recognizes the HIF-responsive elements in a number of hypoxia-inducible target genes (Bruick & McKnight, 2001;Hirsil€ a et al 2005;Masoud & Lin, 2015;Vasconcelos et al 2016;Takedachi et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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Estimating the post mortem interval (PMI) is still a crucial step in Forensic Pathology. Although several methods are available for assessing the PMI, a precise estimation is still quite unreliable and can be inaccurate. The present study aimed to investigate the immunohistochemical distribution and mRNA expression of hypoxia inducible factor (HIF‐1α) in post mortem gingival tissues to establish a correlation between the presence of HIF‐1α and the time since death, with the final goal of achieving a more accurate PMI estimation. Samples of gingival tissues were obtained from 10 cadavers at different PMIs (1–3 days, 4–5 days and 8–9 days), and were processed for immunohistochemistry and quantitative reverse transcription‐polymerase chain reaction. The results showed a time‐dependent correlation of HIF‐1α protein and its mRNA with different times since death, which suggests that HIF‐1α is a potential marker for PMI estimation. The results showed a high HIF‐1α protein signal that was mainly localized in the stratum basale of the oral mucosa in samples collected at a short PMI (1–3 days). It gradually decreased in samples collected at a medium PMI (4–5 days), but it was not detected in samples collected at a long PMI (8–9 days). These results are in agreement with the mRNA data. These data indicate an interesting potential utility of Forensic Anatomy‐based techniques, such as immunohistochemistry, as important complementary tools to be used in forensic investigations.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

et al. 2018

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“…In response to the hypoxic condition induced by the inflammatory disease, activation of HIF-1-α is increased and mechanisms that correct the hypoxic state are developed 3 . Researches evaluating HIF-1-α levels in healthy and inflamed periodontal tissues have reported that HIF-1-α levels were higher in inflamed periodontal tissues 6 , 36 , 37 It has been reported that hypoxic responses of HIF-1-α in periodontal tissues may produce negative feedback mechanisms in the inflammation 6 . These finding demonstrate the importance of the level of HIF-1-α in infectious periodontal tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Hypoxia-inducible factor −1 alpha (HIF-1-α) is one of the HIF-alfa subunits that are the main sensors of hypoxia 5 . HIF-1-α is the main regulatory protein that provides adaptation to hypoxic conditions 6 . HIF-1-α has been shown to be activated by proinflammatory signals in periodontal cells 7 , 8 …”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats

Sağlam¹,

Alinca²,

Celik

et al. 2020

J. Appl. Oral Sci.

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Objective:The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model.Methodology:Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed.Results:ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively).Conclusion:Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group.

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“…One possible mechanism suggested for the progression of periodontal destruction with excessive occlusal forces is that tooth mobility creates a subgingival environment conducive to overgrowth by periodontal pathogens (8). A possible association with occlusal trauma, microbiota and periodontal inflammation might be hypoxia due to periodontal pathogens such as Porphyromonas gingivalis and Fusobacterium nucleatum (9,10). Furthermore, apart from periodontal infection, hypoxia can lead to inflammation with up-regulating pro-inflammatory mediators such as receptor activator of nuclear factor-kB ligand (RANKL) and interleukin (IL)-1β (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Bruksizm Varlığında ve Yokluğunda, Periodontal Sağlık ve Hastalıkta Vasküler Endotelyal Büyüme Faktörü, Hipoksi ile İndüklenebilir Faktör 1-Alfa ve Bakteriyel Plak Kompozisyonunun Değerlendirilmesi: Randomize Olmayan Klinik Çalışma

Akbulut

Karataş

Yüce

et al. 2021

Anadolu Kliniği Tıp Bilimleri Dergisi

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In this study, we aimed to evaluate the possible changes in hypoxia markers and bacterial components in patients with periodontitis and bruxism. Methods: Four study groups were created: 20 healthy individuals without bruxism (Group 1), 20 periodontitis patients without bruxism (Group 2), 20 healthy individuals with bruxism (Group 3), and 20 periodontitis patients with bruxism (Group 4). Plaque index, gingival index, and clinical attachment levels were recorded and gingival crevicular fluid (GCF) and dental plaque samples were taken. The GCF vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1-alpha (HIF-1α) levels were determined. Subgingival plaque samples were evaluated for 5 bacterial species (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Fusobacterium nucleatum). Results: Compared to patients with periodontitis, plaque index, gingival index, and clinical attachment levels were significantly lower in healthy individuals. The GCF and serum levels of VEGF and HIF-1α were similar (p>0.05). The A. actinomycetemcomitans, T. denticola, and F. nucleatum counts did not differ significantly between the study groups (p>0.05). P. gingivalis counts were lowest in Group 1 and increased with both periodontitis and bruxism. T. forsythia counts were higher in Group 1 and Group 2 than in Group 3 and Group 4. The levels in Group 1 were lower than those in Group 2. Conclusion: Individuals with and without bruxism were found to be similar in terms of hypoxia in periodontal health and disease. However, one of the major oral pathogens, P. gingivalis, is affected by the presence of bruxism, regardless of periodontitis.

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Hypoxia‐inducible factor‐1α inhibits interleukin‐6 and ‐8 production in gingival epithelial cells during hypoxia

Cited by 18 publications

References 38 publications

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

HIF1α protein and mRNA expression as a new marker for post mortem interval estimation in human gingival tissue

Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats

Bruksizm Varlığında ve Yokluğunda, Periodontal Sağlık ve Hastalıkta Vasküler Endotelyal Büyüme Faktörü, Hipoksi ile İndüklenebilir Faktör 1-Alfa ve Bakteriyel Plak Kompozisyonunun Değerlendirilmesi: Randomize Olmayan Klinik Çalışma

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