Hypoxia Specific SDF-1 Expression by Retinal Pigment Epithelium Initiates Bone Marrow-derived Cells to Participate in Choroidal Neovascularization in a Laser-induced Mouse Model

Zhang, Zhao Xia; Wang, Yu Sheng; Shi, Yuan; Hou, Hui Yuan; Zhang, Chu; Cai, Yan; Dou, Guo Rui; Yao, Li; Li, Fu Yang

doi:10.3109/02713683.2011.593107

Cited by 34 publications

(31 citation statements)

References 65 publications

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“…Similar to other studies, it was confirmed that CXCR4 and VEGF levels were increased during chemically-induced hypoxia (24,25). Furthermore, it was demonstrated that Res inhibited CXCR4 expression and VEGF secretion, and suppressed HUVEC tube formation.…”

Section: Discussionsupporting

confidence: 88%

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Seong

Ryu

Jeong

et al. 2012

Molecular Medicine Reports

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Abstract. The present study characterizes the effects of resveratrol (Res) on vascular endothelial growth factor (VEGF) secretion in retinal pigment epithelial (RPE) cells. ARPE-19 cells were treated with CoCl 2 , a hypoxia mimetic agent. CoCl 2 treatment increased protein levels of hypoxia inducible factor-1α (HIF-1α) and CXC-chemokine receptor 4 (CXCR4), and secretion of VEGF. To confirm the effects of Res on VEGF secretion, the human umbilical vein endothelial cell tube formation assay was performed with conditioned medium from Res-treated ARPE-19 cells. The well-known antioxidant Res effectively blocked these effects and reduced phosphorylation of nuclear factor (NF)-κB, an upstream activator of CXCR4.

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Section: Discussionsupporting

confidence: 88%

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Seong

Ryu

Jeong

et al. 2012

Molecular Medicine Reports

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“…This may contribute to the migration of peripheral cells into the brain parenchyma [129]. Furthermore, HIF1α is able to induce the expression of SDF-1 via RPE cells [125]. Intraocular insulin-like growth factor-I (IGF-I), but not systemic IGF-I, was sufficient to lead to the breakdown of the BRB according to a study that used transgenic mice which overexpressed IGF-1 in the retina.…”

Section: Pathological Conditions and Chemotherapies Induce The Infiltmentioning

confidence: 98%

“…In mouse models of CNV, hypoxia-induced SDF-1 expressed by the RPE formed a gradient and induced the recruitment of BM-derived cells to the CNV [125,126]. To investigate whether SDF-1 induces the infiltration of BM-derived cells to the retina, Sasahara injected anti-SDF-1 neutralizing antibody into the vitreous of rd1 mice and identified a significant decrease of BM-derived cells compared with the control group [15].…”

Section: Pathological Conditions and Chemotherapies Induce The Infiltmentioning

confidence: 99%

Friend or Foe? Resident Microglia vs Bone Marrow-Derived Microglia and Their Roles in the Retinal Degeneration

et al. 2016

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Microglia are immune cells in the central nervous system (CNS) that originate from the yolk sac in an embryo. The renewal of the microglia pool in the adult eye consists of two components. In addition to the self-proliferation of resident cells, microglia in the CNS also derive from the bone marrow (BM). BM-derived cells pass through the blood-brain barrier (BBB) or blood-retina barrier (BRB) and differentiate into microglia under specific conditions which involves a complex mechanism. Recent studies have widely investigated the role of resident microglia and BM-derived microglia in the retinal degenerative disease. Both two cell types play dual roles and share many similar functions. However, resident microglia tend to polarize to the M1 phenotype which is pro-inflammatory and neurotoxic, whereas BM-derived microglia mainly polarize to the neuroprotective M2 phenotype in retinal degeneration. The molecular mechanism that underlines the invasion of peripheral cells has led to extensive discussions. In addition to the BBB and BRB disruption, many signaling pathways are involved in this process. Based on these studies, we discuss the roles of these two types of microglia in retinal degeneration disease and the potential clinical application of BM-derived microglia, which may benefit future therapies.

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“…CoCl 2 is a chemical hypoxic agent, which can induce the secretion of endogenous VEGF 165 as reported. 24,25 As shown in Figure 7, in CoCl 2 -treated groups, Sema3A showed no effect on VEGF 165 and PEDF secretion (compared with the CoCl 2 -treated group), however, Sema3A inhibited endogenous free VEGF 165 utilization ability (Fig. 7).…”

Section: Effects Of Sema3a On Vegf 165 and Pedf Secretion And Utilizamentioning

confidence: 99%

“…24,25 Sema3A (250 ng/mL and 500 ng/mL) was incubated with RPE in 96-well plates for 24, 48, and 72 hours with or without CoCl 2 . At indicated time-points, the cell culture supernatant were collected to measure VEGF 165 and PEDF concentration.…”

Section: Rna Extraction and Real-time Pcr Of Vegfr2 And Nrp1mentioning

confidence: 99%

Effects of Semaphorin 3A on Retinal Pigment Epithelial Cell Activity

Bai

Han

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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Hypoxia Specific SDF-1 Expression by Retinal Pigment Epithelium Initiates Bone Marrow-derived Cells to Participate in Choroidal Neovascularization in a Laser-induced Mouse Model

Cited by 34 publications

References 65 publications

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Resveratrol suppresses vascular endothelial growth factor secretion via inhibition of CXC-chemokine receptor 4 expression in ARPE-19 cells

Friend or Foe? Resident Microglia vs Bone Marrow-Derived Microglia and Their Roles in the Retinal Degeneration

Effects of Semaphorin 3A on Retinal Pigment Epithelial Cell Activity

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