Hypoxia stress affects the physiological responses, apoptosis and innate immunity of Kuruma shrimp, Marsupenaeus japonicus

Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

“…In the current study, mitochondrial cytochrome c oxidase (mtCOX), mitochondrial manganese superoxide dismutase (mtMnSOD), and glutathione S -transferase θ1 (GST-θ1) in FC/LC and DC/FC comparisons were collectively downregulated. Thulasi et al reported that COX and mitochondrial-like proteins could be associated with mitochondrial membrane damage due to the high levels of ROS production under severe oxidative stress induced by food deprivation. , The mtMnSODs, key antioxidant enzymes that contain manganese, can catalyze anion disproportionation and have been reported to be involved in the innate immune response of crabs. , They are considered the first line of defense against oxidative stress induced by excessive ROS beyond the threshold limits resulting from respiratory bursts . A GST catalyzes the conjugation of glutathione (GSH) with toxic reactive metabolites and is considered a detoxification enzyme against the chemotoxicity of xenobiotics .…”

“…A GST catalyzes the conjugation of glutathione (GSH) with toxic reactive metabolites and is considered a detoxification enzyme against the chemotoxicity of xenobiotics . Moreover, GST-θ was assumed to eliminate the ROS and reduce the oxidative damage owing to its specific serine residue. , The regulation of these DEPs in crab muscle indicated oxidative stress in crabs caused by excess production of ROS during transportation due to severe temperature fluctuations, anoxic environments, and bacterial infections …”

“…32,33 The mtMnSODs, key antioxidant enzymes that contain manganese, can catalyze anion disproportionation and have been reported to be involved in the innate immune response of crabs. 34,35 They are considered the first line of defense against oxidative stress induced by excessive ROS beyond the threshold limits resulting from respiratory bursts. 36 A GST catalyzes the conjugation of glutathione (GSH) with toxic reactive metabolites and is considered a detoxification enzyme against the chemotoxicity of xenobiotics.…”

“…78 Additionally, the TCA cycle enhanced the immune response in crabs, indicating the activation of immune and antioxidation functions in crabs during the first stage of transportation. 35 The upregulation of the mineral absorption pathway might indicate that the metabolism and degradation of nutrients and immune responses were promoted in crabs during the early stage of transportation. However, the upregulation of the peroxisome and downregulation of the chemical carcinogenesis-ROS pathway during the early stage of transportation were attributable to oxidative stress in crabs, embodying the response of the antioxidant mechanism.…”

“…37 Moreover, GST-θ was assumed to eliminate the ROS and reduce the oxidative damage owing to its specific serine residue. 35,38 The regulation of these DEPs in crab muscle indicated oxidative stress in crabs caused by excess production of ROS during transportation due to severe temperature fluctuations, anoxic environments, and bacterial infections. 39 were upregulated in FC/LC, DC/LC, and DC/FC comparisons.…”