Hypoxia-triggered single molecule probe for high-contrast NIR II/PA tumor imaging and robust photothermal therapy

Meng, Xianghe; Zhang, Jiali; Sun, Zhihong; Zhou, Lihua; Deng, Guanjun; Li, Sanpeng; Li, Wenjun; Gong, Ping; Cai, Lintao

doi:10.7150/thno.26607

Cited by 195 publications

(158 citation statements)

References 60 publications

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“…With regards to the potential use of hypoxia probes in the clinic, Image-iT TM works within the NIR I region, thus limiting the depth and localization of the fluorescence source due to biological interference [34], and it is only used pre-clinically. However, a new generation of hypoxia probes are currently under development to operate within the NIR II region (1000 -1700 nm), drastically improving in vivo spatial resolution, signal-to-noise ratio and tissue penetration [35]. Also, current developments in clinical photoacoustic imaging may soon allow the use of such probes in the clinic [35].…”

Section: Discussionmentioning

confidence: 99%

Overcoming hypoxia-induced chemoresistance to cisplatin through tumor oxygenation monitored by optical imaging

et al. 2019

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Perfluorocarbon nanoparticles have been reported to deliver oxygen to tumors and reduce hypoxia-induced radioresistance, however few studies have been carried out to study its role in reducing hypoxia-induced chemoresistance. The oxygenation effect also varies dramatically between different perfluorocarbon formulations and protocols, and there have been no efficient tools to monitor dynamic changes of tumor oxygenation non-invasively. Our goal was to promote tumor oxygenation using perfluorooctyl bromide (PFOB) nanoemulsion and to assess its role in sensitizing tumors to cisplatin treatment. A novel optical imaging protocol was also created to monitor the dynamic changes of tumor oxygenation in real-time. Methods: PFOB nanoemulsion with high oxygen-carrying capacity was prepared and administered to tumor-bearing mice intravenously. Tumor oxygenation was monitored using optical imaging with a hypoxia probe injected intratumorally, thus the oxygenation dynamics and best oxygenation protocol were determined. Various treatment groups were studied, and the tumor growth was monitored to evaluate the role of oxygenation in sensitizing tumors to cisplatin treatment. Results: PFOB nanoemulsion with and without pre-oxygenation along with carbogen breathing resulted in much better tumor oxygenation compared to carbogen breathing alone, while PFOB with air breathing did not show significant increase in tumor oxygenation. Pre-oxygenated PFOB with carbogen breathing produced the most effective oxygenation as early as 5 min post administration. In vitro and in vivo data showed preoxygenated PFOB nanoemulsion with carbogen breathing could increase cisplatin-mediated apoptosis of cancer cells and inhibited tumor growth at a low dose of cisplatin (1 mg/kg) treatment. Furthermore, the treatment did not induce nephrotoxicity. Conclusions: Preoxygenated PFOB nanoemulsion with carbogen breathing can effectively increase tumor oxygenation, which has a great potential to prevent/overcome hypoxia-induced chemotherapy resistance. In addition, optical imaging with intratumoral injection of the hypoxia probe was an efficient tool to monitor tumor oxygenation dynamics during PFOB administration, providing better understanding on oxygenation effects under different protocols.

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Section: Discussionmentioning

confidence: 99%

Overcoming hypoxia-induced chemoresistance to cisplatin through tumor oxygenation monitored by optical imaging

et al. 2019

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“…The NIR‐II QY of FD‐1080 was tested to be 0.31% and could be elevated to 5.94% in FBS buffer. Increasing cyanine NIR‐II dyes have appeared in academic literature through structure redesigning or modifying …”

Section: Synthesis and Chemical Structures Of Nir Dyesmentioning

confidence: 99%

Near‐Infrared‐II Molecular Dyes for Cancer Imaging and Surgery

et al. 2019

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Fluorescence bioimaging affords a vital tool for both researchers and surgeons to molecularly target a variety of biological tissues and processes. This review focuses on summarizing organic dyes emitting at a biological transparency window termed the near‐infrared‐II (NIR‐II) window, where minimal light interaction with the surrounding tissues allows photons to travel nearly unperturbed throughout the body. NIR‐II fluorescence imaging overcomes the penetration/contrast bottleneck of imaging in the visible region, making it a remarkable modality for early diagnosis of cancer and highly sensitive tumor surgery. Due to their convenient bioconjugation with peptides/antibodies, NIR‐II molecular dyes are desirable candidates for targeted cancer imaging, significantly overcoming the autofluorescence/scattering issues for deep tissue molecular imaging. To promote the clinical translation of NIR‐II bioimaging, advancements in the high‐performance small molecule–derived probes are critically important. Here, molecules with clinical potential for NIR‐II imaging are discussed, summarizing the synthesis and chemical structures of NIR‐II dyes, chemical and optical properties of NIR‐II dyes, bioconjugation and biological behavior of NIR‐II dyes, whole body imaging with NIR‐II dyes for cancer detection and surgery, as well as NIR‐II fluorescence microscopy imaging. A key perspective on the direction of NIR‐II molecular dyes for cancer imaging and surgery is also discussed.

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“…Nowadays, PTT has been widely used for tumor treatment by using various photothermal agents, such as Au nanomaterials, organic dyes and polymer semiconductors. [ 50,51,67,66,118 ] In 2019, Yan and Wang et al developed a macromolecular fluorophore (PF) nanoparticle using an amphiphilic polypeptide‐conjugated NIR‐II fluorophore (Flav7) ( Figure a–g). [ 118 ] The synthesized PF nanoparticles showed satisfactory photothermal conversion efficiency (42.3%) and excellent photothermal stability.…”

Section: Advanced Nir Light For Spatiotemporal Modulation Of Cell Funmentioning

confidence: 99%

“…Recently, the dual‐functional strategies have been successfully applied in cancer diagnosis and therapy. [ 67,66 ]…”

Section: Nir Light For Simultaneous Cell Sensing and Modulationmentioning

confidence: 99%

Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

et al. 2020

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Light‐based technique, including optical imaging and photoregulation, has become one of the most important tools for both fundamental research and clinical practice, such as cell signal sensing, cancer diagnosis, tissue engineering, drug delivery, visual regulation, neuromodulation, and disease treatment. In particular, low energy near‐infrared (NIR, 700–1700 nm) light possesses lower phototoxicity and higher tissue penetration depth in living systems as compared with ultraviolet/visible light, making it a promising tool for in vivo applications. Currently, the NIR light‐based imaging and photoregulation strategies have offered a possibility to real‐time sense and/or modulate specific cellular events in deep tissues with subcellular accuracy. Herein, the recent progress with respect to NIR light for monitoring and modulating the spatiotemporal dynamics of cell functions in living systems are summarized. In particular, the applications of NIR light‐based techniques in cancer theranostics, regenerative medicine, and neuroscience research are systematically introduced and discussed. In addition, the challenges and prospects for NIR light‐based cell sensing and regulating techniques are comprehensively discussed.

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Hypoxia-triggered single molecule probe for high-contrast NIR II/PA tumor imaging and robust photothermal therapy

Cited by 195 publications

References 60 publications

Overcoming hypoxia-induced chemoresistance to cisplatin through tumor oxygenation monitored by optical imaging

Overcoming hypoxia-induced chemoresistance to cisplatin through tumor oxygenation monitored by optical imaging

Near‐Infrared‐II Molecular Dyes for Cancer Imaging and Surgery

Advanced Near‐Infrared Light for Monitoring and Modulating the Spatiotemporal Dynamics of Cell Functions in Living Systems

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