Hypoxic conditioned medium of placenta-derived mesenchymal stem cells protects against scar formation

Du, Lili; Lv, Runxiao; Yang, Xiaoyi; Cheng, Shaohang; Ma, Tiantian; Xu, Jing

doi:10.1016/j.lfs.2016.02.050

Cited by 30 publications

(29 citation statements)

References 33 publications

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“…Hypoxic culture conditions have been reported to upregulate HIF-1α and cell proliferation, and increased HIF-1α protein expression has been associated with an increased expression of paracrine factors in stem cells. 29 In this study, hypoxia-induced CM containing paracrine factors were concentrated about 25-fold (hCM).…”

Section: Discussionmentioning

confidence: 92%

The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries

et al. 2019

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Objective This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process. Methods The vocal fold was injured in Sprague‐Dawley rats. Next, hCM from hTMSC (the hCM group) or hTMSC (the hTMSC group) were injected into the injured vocal folds. As a control, saline (the phosphate‐buffered saline group) or 25‐fold concentrated media (the media group) was injected in the same manner. The vocal folds were harvested for quantitative real‐time polymerase chain reaction (PCR) at 1 week and 2 weeks after injury. Histologic evaluation was performed at 3 weeks postinjury. Results In the hCM group at 1 week after injury, PCR showed that the genes encoding hyaluronan synthase (HAS), HAS 1, and HAS 2 were significantly upregulated compared to the media and normal groups. The gene encoding procollagen III was significantly downregulated compared to the media group. Nearly identical results were obtained for the hTMSC group at 1 week after injury. Histological examination showed that the hCM group was similar to or better than the hTMSC group in collagen deposition and hyaluronic acid production. Conclusion The injection of hCM into injured vocal folds produced antifibrotic effects in the early phase of wound healing. These effects were equivalent to those produced by the injection of hTMSC. These results provide a foundation for the future clinical use of hCM for vocal fold regeneration. Level of Evidence NA Laryngoscope, 129:1867–1875, 2019

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Section: Discussionmentioning

confidence: 92%

The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries

et al. 2019

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“…It has been reported that hypoxia preconditioning can enhance the tissue repair ability of MSCs [ 39 ], and activation of HIF-1 α has been considered as an efficient strategy to promote wound repair [ 21 ]. Moreover, conditioned medium from hypoxic MSCs can also accelerate wound healing [ 40 – 42 ]. So we asked whether exposing MSC sheets to chemical hypoxia could improve the wound healing efficacy of their derived ECMs.…”

Section: Discussionmentioning

confidence: 99%

Growth Factor-Reinforced ECM Fabricated from Chemically Hypoxic MSC Sheet with Improved In Vivo Wound Repair Activity

Jiang

Wang

et al. 2017

BioMed Research International

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MSC treatment can promote cutaneous wound repair through multiple mechanisms, and paracrine mediators secreted by MSC are responsible for most of its therapeutic benefits. Recently, MSC sheet composed of live MSCs and their secreted ECMs was reported to promote wound healing; however, whether its ECM alone could accelerate wound closure remained unknown. In this study, Nc-ECM and Cc-ECM were prepared from nonconditioned and CoCl2-conditioned MSC sheets, respectively, and their wound healing properties were evaluated in a mouse model of full-thickness skin defect. Our results showed that Nc-ECM can significantly promote wound repair through early adipocyte recruitment, rapid reepithelialization, enhanced granulation tissue growth, and augmented angiogenesis. Moreover, conditioning of MSC sheet with CoCl2 dramatically enriched its ECM with collagen I, collagen III, TGF-β1, VEGF, and bFGF via activation of HIF-1α and hence remarkably improved its ECM's in vivo wound healing potency. All the Cc-ECM-treated wounds completely healed on day 7, while Nc-ECM-treated wounds healed about 85.0% ± 8.6%, and no-treatment wounds only healed 69.8% ± 9.6% (p < 0.05). Therefore, we believe that such growth factor-reinforced ECM fabricated from chemically hypoxic MSC sheet has the potential for clinical translation and will lead to a MSC-derived, cost-effective, bankable biomaterial for wound management.

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“…I postulate that open wounds are exposed to much higher concentrations of oxygen, which favours TGFB1 and TGFB2, whereas the much lower normal oxygen tensions of tissue in needled skin favour TGFB3. The more anoxic the skin, that is, the bluer the skin looks after skin needling, the better are the results, as anoxia enhances growth factors 22 and possibly the effects of TGFB3 and also IL10 23–25 …”

Section: The Intervals Between Needlingmentioning

confidence: 99%