Hypoxic Induction of Endothelial Cell Growth Factors in Retinal Cells

Shima, David T.; Adamis, A.P.; Ferrara, N; Yeo, Kiang-Teck; Yeo, Tet Kin; Allende, Rafael; Folkman, Judah; amore, P. A.

doi:10.1097/00006982-199616020-00028

Cited by 5 publications

(3 citation statements)

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“…Total cellular RNA (10 μg per sample) was analyzed by Northern blot as previously described (Shima et al, 1995a). Membranes were hybridized with cDNA specific for human PDGF‐B (courtesy of Dr. Mark Mercola, Burnham Institute, La Jolla, CA), rat fibronectin and fibronectin ED‐B (courtesy of Dr. Richard Hynes, MIT, Cambridge, MA), rat cx40 (courtesy of Dr. Klaus Willecke, Universität Bonn, Germany), and bovine 28S rRNA.…”

Section: Methodsmentioning

confidence: 99%

Culture of large vessel endothelial cells on floating collagen gels promotes a phenotype characteristic of endothelium in vivo

2004

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Section: Methodsmentioning

confidence: 99%

Culture of large vessel endothelial cells on floating collagen gels promotes a phenotype characteristic of endothelium in vivo

2004

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“…New blood vessel growth is regulated by a wellorchestrated balance between pro-and anti-angiogenic factors (1). Since endothelial cells in normal adult tissues are quiescent, initiation of angiogenesis requires activation of a process mediated in most forms of post-natal angiogenesis by release of endothelial cell mitogens, such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), from ischemic tissues (2). Pioneering work by Folkman and colleagues has shown that tumors cannot grow beyond 1-2 mm in size in the absence of the ability to promote ingrowth of new blood vessels (3).…”

Section: Introductionmentioning

confidence: 99%

CD36 A critical anti angiogenic receptor

Simantov

Silverstein²

2003

Front Biosci

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Thrombospondin-1 (TSP-1) is a potent inhibitor of angiogenesis in vivo and of microvascular endothelial cell responses to angiogenic factors in vitro. CD36 is the cellular receptor for TSP-1 on microvascular endothelium and is necessary for its anti-angiogenic activity. The anti-angiogenic activity of TSP-1 is contained in a structural domain known as the TSP type I repeat (TSR-1). TSR-1 domains occur in many other proteins, some of which have also been shown to have anti-angiogenic activity. Structure-function analyses have determined that binding of TSP-1 to CD36 is mediated by interaction of the TSR-1 domain of TSP with a conserved domain called CLESH-1 in CD36. Histidine rich glycoprotein, a plasma and cellular protein that blocks the binding of thrombospndin-1 to CD36, inhibits the antiangiogenic response to thrombospondin and may serve to modulate the thrombospondin/CD36 anti-angiogenic pathway. Several in vivo models support the role of the TSP/CD36 system in angiogenesis and tumor growth and provide evidence that the CD36 antiangiogenic pathway offers attractive therapeutic targets.

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“…Hypoxia is a key regulator of VEGF gene expression, both in vitro and in vivo. Hypoxia induces transcription of the VEGF gene 21 , 22 ) . In the VEGF gene, a 28 bp hypoxia response element (HRE) is located approximately 1 kb upstream of the transcription initiation site 23 ) .…”

Section: Introductionmentioning

confidence: 99%

Role of VEGF in Kidney Development, Microvascular Maintenance and Pathophysiology of Renal Disease

Kim

Goligorsky

2003

Korean J Intern Med

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Vascular endothelial growth factor, VEGF, is essential for endothelial cell differentiation (vasculogenesis) and for the sprouting of new capillaries from preexisting vessels (angiogenesis). In addition, there is strong evidence that VEGF is a survival factor allowing the cells to survive and proliferate under conditions of extreme stress.Hypoxia is a key regulator of VEGF gene expression. Besides hypoxia, many cytokines, hormones and growth factors can up-regulate VEGF mRNA expression in various cell types.VEGF is present in the glomerulus of both the fetal and adult kidney. The VEGF produced by glomerular epithelial cell may be responsible for maintenance of the fenestrated phenotype of glomerular epithelial cells, thus facilitating the high rate of glomerular ultrafiltration. But there is little known about the role of VEGF in the tubule.VEGF is thought to be involved in many kinds of kidney diseases. Whereas VEGF has a beneficial role in the pathogenesis in some diseases, it does harmful action in others. Because VEGF is known to be associated with the pathogenesis of some diseases, such as diabetic nephropathy, renal tumor and polycystic kidney disease, the study about the role of VEGF is going to be a target for disease control. On the other hand, an attempt at enhancing the role of VEGF has to be made at diseases like several ARF models and experimental glomerulonephritis.

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Hypoxic Induction of Endothelial Cell Growth Factors in Retinal Cells

Cited by 5 publications

References 0 publications

Culture of large vessel endothelial cells on floating collagen gels promotes a phenotype characteristic of endothelium in vivo

Culture of large vessel endothelial cells on floating collagen gels promotes a phenotype characteristic of endothelium in vivo

CD36 A critical anti angiogenic receptor

Role of VEGF in Kidney Development, Microvascular Maintenance and Pathophysiology of Renal Disease

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