Hyunsoonleella rubra sp. nov., isolated from coastal sediment

Chhetri

International Journal of Systematic and Evolutionary Microbiology

et al. 2021

Two bacterial strains, designated MJB4T and SJ7T, were isolated from water samples collected from Jeongbang Falls on Jeju Island, Republic of Korea. Phylogenetic analysis of 16S rRNA gene sequences indicated that the two strains belonged to the genera Nocardioides and Hyunsoonleella , owing to their high similarities to Nocardioides jensenii DSM 29641T (97.5 %) and Hyunsoonleella rubra FA042 T (96.3 %), respectively. These values are much lower than the gold standard for bacterial species (98.7 %). The average nucleotide identity values between strains MJB4T, SJ7T and the reference strains, Nocardioides jensenii DSM 29641T, Nocardioides daejeonensis MJ31T and Hyunsoonleella flava T58T were 77.2, 75.9 and 75.4 %, respectively. Strains MJB4T and SJ7T and the type strains of the species involved in system incidence have average nucleotide identity and average amino acid threshold values of 60.1–82.6 % for the species boundary (95–96 %), which confirms that strains MJB4T and SJ7T represent two new species of genus Nocardioides and Hyunsoonleella , respectively. Based on phylogenetic and phenotypic data, strains MJB4T and SJ7T are considered to represent novel species of the genus Nocardioides and Hyunsoonleella , respectively, for which the names Nocardioides donggukensis sp. nov. (type strain MJB4T=KACC 21724T=NBRC 114402T) and Hyunsoonleella aquatilis sp. nov., (type strain SJ7T=KACC 21715T=NBRC 114486T) have been proposed.

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nocardioides donggukensis sp. nov. and Hyunsoonleella aquatilis sp. nov., isolated from Jeongbang Waterfall on Jeju Island

Chhetri

International Journal of Systematic and Evolutionary Microbiology

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

“…The genus Hyunsoonleella , a member of the family Flavobacteriaceae , was first proposed by Yoon et al [1], then the genus was amended by Park et al [2] and Shi et al [3]. Up to now, the genus Hyunsoonleella includes five species with validly published names (https://lpsn.dsmz.de/genus/hyunsoonleella), including Hyunsoonleella jejuensis [1], Hyunsoonleella rubra [3], Hyunsoonleella pacifica [4], Hyunsoonleella udonensis [5] and Hyunsoonleella flava [6]. Species of the genus Hyunsoonleella are represented by Gram-stain-negative, non-motile, aerobic, heterotrophic, rod-shaped and yellow to orange bacteria.…”

Section: Introductionmentioning

confidence: 99%

Hyunsoonleella ulvae sp. nov., isolated from algae in China

Wang

Liang

Li³

et al. 2022

A novel Gram-stain-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterial strain, designated HU1-3T, was isolated from Ulva in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HU1-3T represented a member of the genus Hyunsoonleella within the family Flavobacteriaceae , phylum Bacteroidota , and showed the highest 16S rRNA gene sequence similarity to Hyunsoonleella flava T58T (97.6 %). Strain HU1-3T grew at 15–35 °C (optimum, 25–30 °C), pH 6.0–8.0 (optimum, pH 7.0) and in the presence of 2–6 % (w/v) NaCl (optimum, 2–4 %). The draft genome of strain HU1-3T comprised 4.1 Mbp with a G+C content of 33.9mol%. Compared with the reference strain Hyunsoonleella jejuensis CNU004T, the average nucleotide identity value of strain HU1-3T was 77.9 %. The major fatty acids (>10 % of the total) were iso-C15 : 0, iso G-C15 : 1 and iso-C17 : 0 3-OH. MK-6 was the major respiratory quinone. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, and three unknown polar lipids. Many genes that encode glycoside hydrolases (e.g. glucosidase, xylosidase, mannosidase, galactosidase, etc.) were annotated in the genome of strain HU1-3T, which indicated that it might have the ability to degrade various kinds of polysaccharides. Given the phylogenetic, chemotaxonomic, biochemical and genomic data, strain HU1-3T is considered to represent a novel species of the genus Hyunsoonleella , for which the name Hyunsoonleella ulvae sp. nov. is proposed. The type strain is HU1-3T (=KCTC 82511T=MCCC 1K05798T).

Antonie van Leeuwenhoek

Thalassorhabdus aurantiaca gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from seawater in South Korea

Choi

Jang

Cha

et al. 2018

A novel Gram-negative, orange pigmented, strictly aerobic bacterium, designated strain IP9, was isolated from seawater at the sea shore of Incheon Eulwang-ri beach, South Korea. Cells of strain IP9 were observed to be straight or slightly curved rods and colonies to be round and convex. Strain IP9 was found to be catalase and oxidase positive, and non-motile. Growth was observed in the temperature range of 10-37 °C (optimum at 30 °C), pH range of 6-10 (optimum at pH 7-8) and salt concentration range of 0-7% (w/v) NaCl (optimum at 0-1%). On the basis of 16S rRNA gene sequence similarity and phylogenetic analysis, strain IP9 was found to be related to the members of the family Flavobacteriaceae, being closely related to Hwangdonia seohaensis KCTC 32177 (95.3% sequence similarity). The DNA G + C content of the novel strain was determined to be 39.1 mol%. The major polar lipids were found to be phosphatidylethanolamine, three unidentified aminoglycolipids and two unidentified glycolipids. The major fatty acids (> 10%) were identified as iso-C and iso-C 3-OH. The predominant quinone was found to be menaquinone 6 (MK-6). Based on the biochemical, phylogenetic and physiological data, we conclude that strain IP9 (= KCTC 52523 = JCM 31732) represents the type species of a novel genus of the family Flavobacteriaceae for which the name Thalassorhabdus aurantiaca gen. nov., sp. nov. is proposed.